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Full-Text Articles in Life Sciences
Identification Of Soybean Lipoxygenase Products By Gas Chromatography-Mass Spectrometry (Ge-Ms), Rehan Ahmad Malik
Identification Of Soybean Lipoxygenase Products By Gas Chromatography-Mass Spectrometry (Ge-Ms), Rehan Ahmad Malik
Journal of Undergraduate Research at Minnesota State University, Mankato
Lipoxygenases (LOXs) are enzymes which catalyze peroxidation of polyunsaturated fatty acids containing at least one cis, cis 1, 4-pentadiene moiety to form conjugated diene-hydroperoxides. Soybean seeds contain three LOX isoenzymes while at least five different isoenzymes are in the vegetative tissue. Lipoxygenases have been related to several functions i.e. plant growth, defense mechanisms against pathogens and pests, and lipid metabolism. Given the proposed roles of LOX isoenzymes and the presence of multiple LOX isoenzymes in soybean vegetative tissue, it is likely that individual isoenzymes play specific functions. Do the isoenzymes show differences in which products they form? The basic goal …
The Effect Of Maternal Aldosterone Levels On The Expression Of 11b-Hsd Isoenzymes In Normal And Hypertensive Rat Placentae, Nicole Jorissen
The Effect Of Maternal Aldosterone Levels On The Expression Of 11b-Hsd Isoenzymes In Normal And Hypertensive Rat Placentae, Nicole Jorissen
Journal of Undergraduate Research at Minnesota State University, Mankato
In this project, we developed a Western blotting procedure to semi-quantitate levels of 11β-HSD1 and 11β-HSD2 in whole cell extracts. Then, we applied this technique to analyze the effect of reduced maternal aldosterone levels on the expression of 11β-HSD1 and 11β-HSD2 isoenzymes in the placental tissue in both normal and hypertensive rats. These enzymes control levels of glucocorticoids which compete for aldosterone’s mineralocorticoid receptor. Overstimulation of this receptor results in hypertension. If aldosterone levels decrease, levels of the enzymes controlling active glucocorticoid concentrations might change to compensate for the lowered aldosterone levels. Decreased placental 11β-HSD2 expression could affect hypertension in …