Life Sciences Commons^™

Characterizing The Dynamic Localization Of Cmi In Early Drosophila Development, Asra Habibullah

Master's Theses

The COMPASS-like family of lysine methyltransferases, MLR/MLX complexes, are epigenetic regulators that are essential for normal development through the methylation of the fourth lysine residue on histone 3 (H3K4), a universal epigenetic mark associated with active transcription. This family of complexes is highly conserved from yeast to mammals and the genes encoding the human MLR complexes have been associated with various developmental diseases and cancers (Dingwall and Fagan, 2019). In D. melanogaster, the enzymatic methyltransferase core of this complex is composed of two proteins: Cara Mitad (Cmi, also known as Lpt) and Trithorax-related (Trr). Although these proteins have been shown …

The Impacts Of Framework Mutations On Steady-State Kinetics Of An Aldolase Abzyme, Charles Andrew Mettler

Master's Theses

During affinity maturation, antibodies acquire point mutations that lead to enhanced binding strength to a particular ligand while simultaneously becoming more conformationally restricted as they become increasingly complementary to their ligand’s electrostatic geometry. This property has catalytic potential in transition-state (TS) stabilization whereby kinetic barriers (i.e., the energetic gap between the substrate and its TS) are reduced and reaction rates are enhanced. This basic mechanism is thought to underlie the majority of the rate enhancement observed for enzymes. A key integration of antibody binding specificity and strength with enzymatic catalytic strategy is the development of catalytic antibodies (“abzymes”). Abzymes are …

Structure-Function Relationship Studies Of The Udp-Glucose Pyrophosphorylase From Escherichia Coli, Agnieszka Maria Orlof

Master's Theses

UDP-Glucose Pyrophosphorylase (UDP-Glc PPase) is a key enzyme of the carbohydrate metabolic pathway widely used among prokaryotes and eukaryotes. In plants, UDP-Glc PPase is necessary for sucrose synthesis while mammals utilize this enzyme for the production of glycogen. A bacterium such as Escherichia coli uses UDP-Glc PPase for biosynthesis of the lipopolysscharide core which forms a cell wall.

This study focused on UDP-Glc PPase from Escherichia coli which is encoded by both galU and galF genes. The first part of this project investigated amino acids that could play an important role in the function of UDP Glc PPase (GalU). Based …

The Endocytosis Of Iodinated Protein Substrates In Cultured Human Skin Fibroblasts, Daniel P. Wilcockson

Master's Theses

No abstract provided.

The Purification, Properties And Chemical Kinetics Of Cathepsin B1, Andrew Stanley Bajkowski

Master's Theses

No abstract provided.

Aldehydic Transition State Analogue Inhibitors Of Α-Chymotrypsin, Alexandra Constance Cheerva

Master's Theses

No abstract provided.

Reduction Products Of D-Glucoheptulose, Stanley John Kuman

Master's Theses

No abstract provided.

Conjugation Of Various Amino Acids With Insulin In Liquid Ammonia, Charles Jon Caul

Master's Theses

No abstract provided.

Reactions Of D-Glucuronic Acid And Its Derivatives, Edward John Kubicz

Master's Theses

No abstract provided.

Reactions Of Synthesis Of Methyl D-Glucuronide, Edward J. Czalgoszerski

Master's Theses

No abstract provided.

Effect Of Calcium Chloride, Acid, And Alkali On Irritability And Conductivity Of Nerves Locally Cooled, Dominic Baima

Master's Theses

No abstract provided.