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Full-Text Articles in Life Sciences
A Label-Free Assay For Aminoacylation Of Trna, Howard Gamper, Ya-Ming Hou
A Label-Free Assay For Aminoacylation Of Trna, Howard Gamper, Ya-Ming Hou
Department of Biochemistry and Molecular Biology Faculty Papers
Aminoacylation of tRNA generates an aminoacyl-tRNA (aa-tRNA) that is active for protein synthesis on the ribosome. Quantification of aminoacylation of tRNA is critical to understand the mechanism of specificity and the flux of the aa-tRNA into the protein synthesis machinery, which determines the rate of cell growth. Traditional assays for the quantification of tRNA aminoacylation involve radioactivity, either with a radioactive amino acid or with a [3'-32P]-labeled tRNA. We describe here a label-free assay that monitors aminoacylation by biotinylation-streptavidin (SA) conjugation to the α-amine or the α-imine of the aminoacyl group on the aa-tRNA. The conjugated aa-tRNA product is readily …
Aminoacyl-Trna Synthetases, Miguel Angel Rubio Gomez, Michael Ibba
Aminoacyl-Trna Synthetases, Miguel Angel Rubio Gomez, Michael Ibba
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
The aminoacyl-tRNA synthetases are an essential and universally distributed family of enzymes that plays a critical role in protein synthesis, pairing tRNAs with their cognate amino acids for decoding mRNAs according to the genetic code. Synthetases help to ensure accurate translation of the genetic code by using both highly accurate cognate substrate recognition and stringent proofreading of noncognate products. While alterations in the quality control mechanisms of synthetases are generally detrimental to cellular viability, recent studies suggest that in some instances such changes facilitate adaption to stress conditions. Beyond their central role in translation, synthetases are also emerging as key …