Life Sciences Commons^™

Determining The Binding Between Saga Subunits And Spliceosomal Components, Peyton J. Spreacker, Rachel L. Stegeman, Vikki M. Weake

The Summer Undergraduate Research Fellowship (SURF) Symposium

Proper gene regulation is vital to the health and development of an organism. Determining the relationship between splicing, transcription, and chromatin structure is vital for understanding gene regulation as a whole. There have been previous studies linking these elements pairwise; however, no evidence exists for a direct link between all three. Recent data shows that splicing components of the U2 small nuclear ribonucleic protein (snRNP) co-purify with Spt-Ada-Gcn5-acetyltransferase (SAGA), a highly conserved transcriptional co-activator and chromatin modifier. We hypothesize that SAGA binds with splicing components through a multi-protein binding surface with certain core components based on preliminary yeast two-hybrid data. …

The Effect Of Transient Hmg-Coa Reductase And 1-Deoxy-D-Xylulose-5-Phosphate Synthase Overexpression On Terpene Production In Transgenic Tomato Fruits, Scott A. Gentry, Michael Gutensohn, Laura Henry, Natalia Dudareva

The Summer Undergraduate Research Fellowship (SURF) Symposium

Isoprenoids are secondary metabolites that control numerous plant functions including signaling, growth, photosynthesis, and membrane structure. The bioengineering of isoprenoid synthesis could produce plants with a variety of beneficial traits. Plants form isoprenoids using two different pathways, the mevalonate (MVA) pathway and the methylerithritol phosphate (MEP) pathway, which cooperate via metabolic cross-talk. Transgenic tomato lines expressing both the plastidic and cytosolic forms of the snapdragon nerolidol/linalool terpene synthase under a fruit ripening specific promoter were transiently transformed to overexpress key enzymes in the two isoprenoid pathways. Hydroxymethylglutaryl-coenzyme A reductase (HMGR) is the rate limiting enzyme in the MVA pathway that …

High Level Protein Expression Slows Down Rate Of Growth Of Bacterial Broth Cultures, Saad Chaudhry, Kenechukwu Ekwemalor, Shobhit Keswani, Odutayo Odunuge

Undergraduate Research Conference

No abstract provided.

Determination Of Anions In Lanana Creek By Ion Chromatography, Tania Benavides

Undergraduate Research Conference

Abstract

Lanana Creek is one of two springs that surround Nacogdoches, TX. Lanana Creek starts southwest of Lake Naconiche, conjoining with several other bodies of water along its path, and becomes part of the Angelina River. This body of water eventually ends in the Gulf of Mexico which may contribute to the dead zone. Contaminants in water may be of small concentration; however, prolonged exposure could produce many negative effects. To prevent further contamination, the source of the contamination must be identified through testing the water for contaminants. Some possible sources of contamination may be from fertilizers and pesticides used …

Localization And Expression Level Of Vascular Endothelial Growth Factor After Partial Hepatectomy Of Wky Rats, Adam Kronebusch, Tyler Koonst

Undergraduate Research Symposium

Inflammation and fibrosis are indicative of liver regeneration following injury and chronic liver diseases, such as cirrhosis and hepatocellular carcinoma. The formation of new vasculature via the process of angiogenesis is vital to the pathological progression of liver regeneration and these diseases. Thus, inhibiting the process of angiogenesis could have the potential to stop or slow the progression of chronic liver diseases. Vascular endothelial growth factor (VEGF) is the most potent and specific growth factor for initiating the process of angiogenesis, making it a prime target to inhibit angiogenesis. Bevacizumab is an antibody that binds to VEGF, inhibiting it from …

Estrogenic Effects On A Protandrous Hermaphroditic Species: Amphiprion Percula, Randall Burns, Katie Dose

Undergraduate Research Symposium

Estrogen concentrations are increased in aquatic environments due to the addition of pharmaceutical drugs (i.e. birth control) through runoff water. This manipulates maturation of sexes within many species that inhabit aquatic enviornments, including fresh and marine habitats. Amphiprion percula exhibit a hierarchal system behavioral pattern, which determines the social status of individuals within a group. Multiple clown anemonefish, A. percula were studied via a controlled environment and hierarchal succession was observed under estrogenic additions. From this study, data on sex change of a hermaphroditic protandrous species was observed under the influence of three concentrations of natural estrogen. Based on sex …

Rubidium Uptake In Isolated Rat Cardiomyocytes, Jooyoung Lee, Jaekook Sim, Karleen Doering

Undergraduate Research Symposium

The element rubidium is known to have similar biological impact as K+ ions and some studies have shown that heart tissue takes up rubidium through K+ channels and Na+/K+ ATPase pumps. The purpose of the research was to confirm whether or not rubidium was transported into individual cardiomyocytes. Rubidium may be used as a marker to study different physiological functions of K+ and its channels and pumps. The methods used are mainly two parts: isolation of single heart cells and treatment with rubidium. Isolated heart cells were obtained by anesthetizing a rat, extracting the heart, and infusing its aorta with …

Effect Of Artificial Sweeteners On The Renin-Angiotensin System In Rats, Jacob Ball

Undergraduate Research Symposium

Normal abundant dietary sugars such as fructose and glucose can contribute to hypertension and other health issues. To avoid these health complications, many individuals use artificial sweeteners. An equivalent intake of some artificial sweeteners also can lead to hypertension. However, Stevia, a sweetener that is isolated from a Paraguayan plant, was shown in relevant literature to decrease blood pressure in both rat specimens and humans. The general purpose of this research project was to study the effect of Stevia and glucose on the expression of two key components of the renin-angiotensin-aldosterone system (RAAS): prorenin receptor (PRR) and angiotensin receptor type …

Novel Mutations That Affect Stomata Development In Arabidopsis Thaliana, John Woodson Marshal Collins, William J. Carmack

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Located on the epidermal surface of plants, stomata are small, pore-like structures that act as channels to exchange gas and water vapor between plant cells and the environment. Concentrations of gases and water within the plant cell are regulated through opening and closing of the stomata by turgor-driven movements. In Arabidopsis thaliana, development and differentiation of cells is controlled by the ERECTA (ER) family of genes (ERECTA, ERL1, and ERL2) which encode leucine-rich repeat-receptor-like kinases (LRR-RLKs). Acting synergistically, they direct cell division in different tissues and formation of stomata in epidermis. To better understand how ERECTA family genes regulate stomata …

Optimizing The Expression Of Nitronate Monooxygenase In E. Coli, Jennifer Hernandez

Georgia State Undergraduate Research Conference

No abstract provided.