Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Spectrophotomatic Assay For The Enzyme Catalyzed Reaction Of 4-Nitroquinoline 1-Oxide With Glutathione, J. Steven Stanley, Ann M. Benson
Spectrophotomatic Assay For The Enzyme Catalyzed Reaction Of 4-Nitroquinoline 1-Oxide With Glutathione, J. Steven Stanley, Ann M. Benson
Journal of the Arkansas Academy of Science
4-Nitroquinoline 1-oxide (4NQO(is a toxic and carcinogenic compound that has been reported to be subject to conjugation with glutathione (GSH). This reaction may proceed non-enzymatically or be catalyzed by GSH transferases. The non-enzymatic rate for this reaction has been reported to be very high. The purposes of this investigation were to develop a spectrophotometric assay for the reaction of4NQO with GSH and to determine whether the rate for the enzyme catalyzed reaction was significant relative to the non-enzymatic reaction. The absorbance spectrum of 4NQO in phosphate buffer exhibited a maximum at 365 nm. Reaction of 4NQO with GSH was accompanied …