Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- 5-HT (1)
- Albumin (1)
- Cancer patients (1)
- Chemotherapy (1)
- Cycl-oxygenase (COX) inhibitors (1)
-
- Granulocyte macrophage-colony stimulating factor (1)
- Granulocyte-colony stimulating factor (1)
- Human (1)
- LDH-5 (1)
- Lecture-based learning (1)
- MAP kinase (1)
- MCQ test scores (1)
- Nitric oxide (1)
- Non-Steroidal anti-inflammatory drugs (1)
- PAF (1)
- Platelet aggregation (1)
- Problem-based learning (PBL) (1)
- Serum (1)
- Stem cells (1)
- Students' test scores (1)
- Synergism (1)
Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Molecular Mechanisms Involved In Human Platelet Aggregation By Synergistic Interaction Of Platelet-Activating Factor And 5-Hydroxytryptamine, B H. Shah, H Rasheed, I H. Rahman, A H. Shariff, F L. Khan, H B. Rahman, S Hanif, S A. Saeed
Molecular Mechanisms Involved In Human Platelet Aggregation By Synergistic Interaction Of Platelet-Activating Factor And 5-Hydroxytryptamine, B H. Shah, H Rasheed, I H. Rahman, A H. Shariff, F L. Khan, H B. Rahman, S Hanif, S A. Saeed
Department of Biological & Biomedical Sciences
Our recent studies have shown that co-activation of Gq and Gi proteins by 5-hydroxytryptamine (5-HT) and adrenaline show synergism in human platelet aggregation. This study was conducted to examine the mechanism(s) of synergistic interaction of 5-HT and platelet activating factor (PAF) in human platelets. We show that PAF, but not 5-HT, increased platelet aggregation in a concentration-dependent manner. However, low concentrations of 5-HT (2 microM) potentiated platelet aggregation induced by subthreshold concentration of PAF (40 nM) indicating a synergistic interaction between the two agonists and this synergism was blocked by receptor antagonists to either 5-HT or PAF. 5-HT also potentiated …
Problem-Based Learning Variant: Transition Phase For A Large Institution, I Khan, A Fareed
Problem-Based Learning Variant: Transition Phase For A Large Institution, I Khan, A Fareed
Department of Biological & Biomedical Sciences
Objective: To compare students' test scores and perceptions of problem-based learning (PBL) and lecture-based learning (LBL) by applying a PBL-variant.
Method: For the transition from LBL to PBL, PBL was varied for one discipline only and for a large group of students. Two hundred forty nine second year medical students were taught a topic of Biochemistry by the LBL method and then 141 of these were taught another topic by the PBL-variant. At the conclusion of each topic an MCQ test was given. One week later a 9 item questionnaire was given to the 50 students now attending classes to …
An Enzymatic Method For The Detection Of Human Serum Albumin, M U. Javed, S N. Waqar
An Enzymatic Method For The Detection Of Human Serum Albumin, M U. Javed, S N. Waqar
Department of Biological & Biomedical Sciences
Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.
Induction Of White Cell Proliferation Due To Haematopoietic Growth Factors Is Associated With An Increase In Multiple Forms Of Dihydrofolate Reductase In Non-Neutropenic Cancer Patients, M P. Iqbal, I A. Burney, F Sultana, N Mehboobali
Induction Of White Cell Proliferation Due To Haematopoietic Growth Factors Is Associated With An Increase In Multiple Forms Of Dihydrofolate Reductase In Non-Neutropenic Cancer Patients, M P. Iqbal, I A. Burney, F Sultana, N Mehboobali
Department of Biological & Biomedical Sciences
Objective: Granulocyte-colony stimulating factor (G-CSF) and granulocyte macrophage-colony stimulating factor (GM-CSF) are frequently used in cancer patients to overcome the granulocytopenic effects of chemotherapy, and also to mobilize the stem cells. The mobilized stem cells are collected from the peripheral blood and used for transplantation following high doses of chemotherapy. However, the molecular mechanism by which these colony stimulating factors (CSFs) bring about proliferation of myeloid precursor cells is not clearly known. Dihydrofolate reductase (DHFR), which has an established role in DNA synthesis, could be a link between administration of CSF and stem cell proliferation. The purpose of this study …
Nitric Oxide Mediated Effect Of Cyclo-Oxygenase Inhibitors, M Mahmood, H S. Zuberi, M K. Ashfaq
Nitric Oxide Mediated Effect Of Cyclo-Oxygenase Inhibitors, M Mahmood, H S. Zuberi, M K. Ashfaq
Department of Biological & Biomedical Sciences
Objectives: Non-Steroidal anti-inflammatory drugs (NSAIDS) have long been used as anti-inflammatory agents, yet their mode of action is not entirely clear. The inhibitory effects of NSAIDS on prostaglandin production can only partly explain their anti-inflammatory actions. This study was aimed at defining the role of cycl-oxygenase (COX) inhibitors on nitric oxide (NO) production in murine macrophages in vitro.
Methods: Murine macrophages were obtained from the peritoneum and after exposure, in vitro to lipopolysaccharide (LPS) produced nitrite, measured after 24 hours by Griess reaction. The macrophages were pre-incubated with aspirin or indomethacin before activation with LPS.
Results: Treatment with aspirin resulted …