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Accumulation Of Methotrexate In Human Tissues Following High-Dose Methotrexate Therapy, M P. Iqbal
Accumulation Of Methotrexate In Human Tissues Following High-Dose Methotrexate Therapy, M P. Iqbal
Department of Biological & Biomedical Sciences
Methotrexate concentration was analyzed in a number of tissues of a patient of osteogenic sarcoma who had been on high-dose methotrexate therapy for nearly 6 months. Gall bladder and kidney contained the highest concentration of the drug, followed by testis, small intestine, skeletal muscle, bone marrow, lung, spleen, heart and liver. Although, compared to kidney the liver contained relatively small amount of the drug, yet nearly 1/5th of the total drug in liver was in bound form. This bound form of methotrexate is most likely associated with multiple forms of dihydrofolate reductase. The total concentration of methotrexate in kidney is …
Urinary N-Acetyl-Beta-D-Glucosaminidase In Rheumatoid Arthritis, M P. Iqbal, A A. Ali, M A. Waqar, N Mehboobali
Urinary N-Acetyl-Beta-D-Glucosaminidase In Rheumatoid Arthritis, M P. Iqbal, A A. Ali, M A. Waqar, N Mehboobali
Department of Biological & Biomedical Sciences
Excretion of urinary N-acetyl beta-D-glucosaminidase (NAG) and its isoenzyme patterns were studied in two groups of patients with rheumatoid arthritis (RA) and in normal control subjects. Urine samples were collected from 30 seropositive RA patients, 19 seronegative RA patients, and 15 normal healthy subjects. All the patients and normal subjects were assessed to have normal liver and kidney functions. A small portion of the urine sample was dialyzed against 0.01 M phosphate buffer, pH 7.0 and NAG activity was monitored. Mean +/- SD values of urinary NAG in seropositive RA patients, in seronegative RA patients and in normal healthy subjects …