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Full-Text Articles in Life Sciences
Modelling, Cloning, And Expression Of The J Domain Of C. Elegans Rme-8 Protein, Madison Thornhill, My Tran, Bingbing Xiao
Modelling, Cloning, And Expression Of The J Domain Of C. Elegans Rme-8 Protein, Madison Thornhill, My Tran, Bingbing Xiao
Undergraduate Research Conference
Rme-8 is a J domain containing plasma membrane protein that is required for endocytosis in various cells The J domain is a characteristic structural motif found mainly in heat shock protein 40 (Hsp 40 or DnaJ) and other proteins such as Rme-8 Within the J domain is a tripeptide, the HPD motif, that is required by the J domain protein to interact with and stimulate the ATPase activity of Hsp70 a major cellular chaperone Rme-8 protein in C elegans CeRme-8 has not been identified with a particular Hsp70 partner CeHsp70 1 is the only cytosolic Hsp70 in C elegans therefore, …
Quantification Of Kaempferol Conjugates In Watercress Juice And Methanol Extract: A Study Using Hplc And Protein Binding, Laken Simington
Quantification Of Kaempferol Conjugates In Watercress Juice And Methanol Extract: A Study Using Hplc And Protein Binding, Laken Simington
Undergraduate Research Conference
Flavonoids are a large group of biologically active polyphenolic compounds found in plants, that have gained importance in recent years due to their potential medicinal and therapeutic properties of high potency and low systemic toxicity. Kaempferol (3,4′,5,7- tetrahydroxyflavone) is a polyphenol antioxidant dietary flavonoid found in a variety of fruits and leafy vegetables, specifically vegetables of the Brassica family, which includes our subject vegetable, watercress (WC) which is usually grown in marshy lands. Kaempferol (KMP) has been depicted to have revolutionary attribute in overall human health ranging from anti-cancerous to anti-inflammatory properties. Here we show a novel approach for quantifying …
Bomb Calorimetry: A New Method Of Cec Determination, Trace Washburn
Bomb Calorimetry: A New Method Of Cec Determination, Trace Washburn
Undergraduate Research Conference
A new method of determining the cation exchange capacity (CEC) of clay minerals was examined. This method involved exchanging the smectite clay with organic cations and using bomb calorimetry to measure the energy of combustion of the material. Because the clay is noncombustible, the energy is completely dependent on the amount of organic present, which is dependent on the CEC of the clay. Two organic cations, tetrapentylammonium and octadecyltrimethylammonium , with similar size but much different shape were used. For clays with lower CECs, using the more symmetric ion yielded better accuracy (4.24%), while for clays with higher CEC, using …
The Transesterification Of Hickory Nuts Into Biodiesel Fuel, Hannah Trauger, Madilynn Dewell, Jahida A. Mendoza
The Transesterification Of Hickory Nuts Into Biodiesel Fuel, Hannah Trauger, Madilynn Dewell, Jahida A. Mendoza
Undergraduate Research Conference
• Biodiesel fuels can be produced from a fat and an alcohol via transesterification
• Hickory nuts have a high fat content (~60 % by weight)
• Can nuts from shagbark hickory (Carya ovata) be used to produce a biodiesel fuel using existing acidcatalyzed transesterification methods?
Longitudinal Study Of Lanana Creek, Bayler Barnes, Jackson T. Ortiz, Trace Washburn
Longitudinal Study Of Lanana Creek, Bayler Barnes, Jackson T. Ortiz, Trace Washburn
Undergraduate Research Conference
A longitudinal study was done to collect data on the various conditions of LananaCreek in Nacogdoches, Texas over a seven week span from September 14 to November 2, 2018. The part of the creek studies is a 3.5 mile stretch from East Austin Street to Hoya Soccer Complex, mostly located on the campus of Stephen F. Austin State University. There were 6 different testing sites selected along this expanse. Collection of samples was performed by the General Chemistry I Honors class as part of the laboratory experience. Once collected, the samples were transported back to the lab for testing. The …
Extraction And Quantification Of Hydrolyzable Tannins In Acorns From Different Species Of Oak Trees (Quercus Spp.), Sarah Browning, Marshall E. Woodruff
Extraction And Quantification Of Hydrolyzable Tannins In Acorns From Different Species Of Oak Trees (Quercus Spp.), Sarah Browning, Marshall E. Woodruff
Undergraduate Research Conference
Do white-tailed deer (Odocoileus virginianus) prefer to eat certain types of acorns based on tannin content?
Hydrolyzable tannins consist of gallate groups connected via ester linkages to a carbohydrate (D-glucose)
The Prussian Blue method was used to determine hydrolyzable tannin content
Cation Exchange Capacity Determination: Comparative Study Of Three Methods, Lillie Zech
Cation Exchange Capacity Determination: Comparative Study Of Three Methods, Lillie Zech
Undergraduate Research Conference
No abstract provided.
Design Of A Robust Undergraduate Biochemistry Laboratory Course Based On A Modified And Expanded Bovine Serum Albumin Purification Scheme, Odutayo O. Odunuge, Nicholas Cheatwood, John Mullins, Michele Harris
Design Of A Robust Undergraduate Biochemistry Laboratory Course Based On A Modified And Expanded Bovine Serum Albumin Purification Scheme, Odutayo O. Odunuge, Nicholas Cheatwood, John Mullins, Michele Harris
Symposium on Arts and Research
The ASBMB curriculum for an undergraduate degree recommends a set of skills that can be acquired only through laboratory courses and research experience. Based on a previously reported purification scheme for BSA (Odunuga and Shazhko, 2013), we designed a robust, reproducible, cost-effective, safe and enquiry-based undergraduate biochemistry laboratory course that encompasses many of the skill-sets recommend-ed in the ASBMB curriculum. Our work not only modi-fied certain steps in the scheme, it also included addi-tional steps to enhance student learning and skill ac-quisition. Salt precipitation, ion exchange and size ex-clusion chromatography were employed by students to purify BSA from cow plasma. …
Antimicrobial Properties Of S-Benzofuran-2-Yl Ethanol Produced By Biotransformation, Nick Cheatwood, Ann Davis, Elizabeth Schabeck, Kelsi Hooker, Michele Harris
Antimicrobial Properties Of S-Benzofuran-2-Yl Ethanol Produced By Biotransformation, Nick Cheatwood, Ann Davis, Elizabeth Schabeck, Kelsi Hooker, Michele Harris
Undergraduate Research Conference
All living organisms contain enzymes to carry out biological reactions, which produce substances with a specific three dimensional shape. Enantiomers are two molecules that are mirror images of each other just as hands are mirror images of each other. It is well known in the pharmaceutical world that one of the enantiomers (one of the mirrorimage molecules) has a positive biological effect while the other can be harmful or have no effect. Currently, any potential pharmaceutical that could exist as enantiomers must have each enantiomer tested for biological activity prior to FDA approval. In our laboratory, we have focused on …
Antimicrobial Properties Of Isomers Of Benzofuranylethanol, Nick Cheatwood, Jessica Bronstad, Michele Harris
Antimicrobial Properties Of Isomers Of Benzofuranylethanol, Nick Cheatwood, Jessica Bronstad, Michele Harris
Undergraduate Research Conference
A biotransformaDon reacDon is a chemical conversion of a substance into a desired product with the aid of whole, living cells containing the necessary enzymes. In addiDons to living cells, other substances, such as vegetable strips, will also catalyze biotransformaDon reacDons. Advantages of biotransformaDon reacDons include the following: the ability to recycle material, the use of less hazardous chemicals, the ability to compost vegetable strips, the use of inexpensive materials that are locally available, and the ability to produce a single isomer. The biotransformaDon reacDon for the conversion of benzofuranyl methyl ketone (BMK) to (-)-benzofuranylethanol (BMA) using carrot strips as …
Water Soluble Cationic Porphyrin Sensor For Detection Of Hg2+, Pb2+, Cd2+, And Cu2+, Christopher Orr, Miranda Uherek, Matibur Zamadar
Water Soluble Cationic Porphyrin Sensor For Detection Of Hg2+, Pb2+, Cd2+, And Cu2+, Christopher Orr, Miranda Uherek, Matibur Zamadar
Undergraduate Research Conference
Here we report the sensing properties of the aqueous solution of mesotetra(N-methyl-4-pyridyl)porphine tetrachloride (1) for simultaneous detection of toxic metal ions by using UV-vis spectroscopy. Cationic porphyrin 1 displayed different electronic absorptions in UV-vis region upon interacting with Hg2+, Pb2+, Cd2+, and Cu2+ ions in neutral water solution at room temperature. Quite interestingly, the porphyrin 1 showed that it can function as a single optical chemical sensor and/ or metal ion receptor capable of detecting two or more toxic metal ions, particularly, Hg2+, Pb2+, and Cd2+ ions coexisting in a water sample. Porphyrin 1 in an aqueous solution provides a …
High Level Protein Expression Slows Down Rate Of Growth Of Bacterial Broth Cultures, Saad Chaudhry, Kenechukwu Ekwemalor, Shobhit Keswani, Odutayo Odunuge
High Level Protein Expression Slows Down Rate Of Growth Of Bacterial Broth Cultures, Saad Chaudhry, Kenechukwu Ekwemalor, Shobhit Keswani, Odutayo Odunuge
Undergraduate Research Conference
No abstract provided.
Determination Of Anions In Lanana Creek By Ion Chromatography, Tania Benavides
Determination Of Anions In Lanana Creek By Ion Chromatography, Tania Benavides
Undergraduate Research Conference
Abstract
Lanana Creek is one of two springs that surround Nacogdoches, TX. Lanana Creek starts southwest of Lake Naconiche, conjoining with several other bodies of water along its path, and becomes part of the Angelina River. This body of water eventually ends in the Gulf of Mexico which may contribute to the dead zone. Contaminants in water may be of small concentration; however, prolonged exposure could produce many negative effects. To prevent further contamination, the source of the contamination must be identified through testing the water for contaminants. Some possible sources of contamination may be from fertilizers and pesticides used …
Differentiation Of Capsicum Species Via Protein Extraction And Sds-Page Analysis: A Two-Day Experiment For Undergraduate Biochemistry Laboratories, Trey Polvadore, Brittney P. Cooper, Michele R. Harris
Differentiation Of Capsicum Species Via Protein Extraction And Sds-Page Analysis: A Two-Day Experiment For Undergraduate Biochemistry Laboratories, Trey Polvadore, Brittney P. Cooper, Michele R. Harris
Undergraduate Research Conference
The Capsicum genus is comprised of more than 200 varieties of pepper plant that collectively represent only five cultivated spices. As a result of extensive cross-breeding, an 88% similarity in protein coding DNA has developed. While this has made species determination based solely on physical appearance all but impossible, the 12% difference in protein coding DNA has led to subtle differences in translated protein. SDS-PAGE analysis facilitates the generation of protein banding patterns that, after careful analysis, was used to differentiate one variety of pepper from another. This project was used to distinguish between pepper plant species and was carried …
Analysis Of Critical Amino Acid Residues In Unc-45 Necessary For Its Interaction With Myosin Using Site-Directed Mutagenesis, Amanda H. Nolan
Analysis Of Critical Amino Acid Residues In Unc-45 Necessary For Its Interaction With Myosin Using Site-Directed Mutagenesis, Amanda H. Nolan
Undergraduate Research Conference
UNC-45 is an important protein for muscle contraction and heart formation. The UNC-45 protein is a chaperone for the myosin heavy chain head, and UNC-45 only binds to the UCS domain of the myosin head. In our research for the interaction of myosin and UNC-45. The mutation was accomplished using the multiple sequence alignment method and site-directed mutagenesis. The mutations were generated in UNC-45 of Mus musculus (mouse), and the specific amino acids that were mutated were: L741A and L860F. The mutant proteins were sequenced and over-expressed for analysis.