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Full-Text Articles in Life Sciences
Purification Of Recombinant E. Coli Topoisomerase Iii For Structure-Based Drug Design Using Protein Crystallization, Miguel A. Perez Rodriguez, Yuk-Ching Tse-Dinh
Purification Of Recombinant E. Coli Topoisomerase Iii For Structure-Based Drug Design Using Protein Crystallization, Miguel A. Perez Rodriguez, Yuk-Ching Tse-Dinh
FIU Undergraduate Research Journal
Type IA Topoisomerases are ubiquitous enzymes found throughout all life forms and species. These topoisomerases relieve the topographical constrains formed by DNA during processes like replication and transcription via a cleavage-religation mechanism performed through a catalytically active tyrosine residue in the primary structure of the enzyme. E. coli Topoisomerase III (EtopIII) is a type of Type IA topoisomerase, and its main function in the cell is as a decatenase, which means that it unlinks circular or intertwined pieces of genetic material and creates two unlinked segments of DNA from a singular linked chain. Structure-based determination of the enzyme’s three-dimensional structure …
Assay Replicability In Β-Glucosidase Enzyme Kinetics Across Laboratories, Nicole-Tia Najam, Jaime Mayoral
Assay Replicability In Β-Glucosidase Enzyme Kinetics Across Laboratories, Nicole-Tia Najam, Jaime Mayoral
FIU Undergraduate Research Journal
Replicability is the foundation of research in any scientific discipline. Despite this fact, few studies address experimental variability within and across multiple institutions that operate under the same protocol. While consistency is usually well documented within the same lab, multi-institutional experiments may introduce new variables and, therefore, variability that may lead to inconsistent results. This study seeks to explore intra- and interinstitutional variability among enzyme catalytic efficiency values (KM and Kcat/KM) for the wild type of β-Glucosidase derived from Paenibacillus polymyxa. A standardized protocol for the assay was provided to all institutions that participated in …