Life Sciences Commons^™

Photobiomodulation Of Bovine Oocytes During Maturation Increases Atp Content And Enhances Subsequent Embryonic Development, Kendall Richey

All Theses

Bovine in-vitro production(IVP) of embryos is a growing field for producing offspring with desirable genetics in the cattle industries. While IVP is effective, oocyte cytoplasmic maturation is compromised, and bovine oocytes matured in-vitro have reduced metabolic activity than those matured in-vivo. Mitochondria are the central unit of oocyte metabolism, producing ATP through OXPHOS. Photobiomodulation is a light treatment reported to improve metabolic activity. In five experiments, we measured the effects of photobiomodulation treatment with red LED at 16-(L-16) and 20 h(L-20) of bovine oocyte in-vitro maturation on subsequent embryonic development, mitochondrial activity and nuclear progression. Bovine COCs were aspirated …

Strategic Nutritional Interventions With Probiotics And Their Relationship To Performance, Feeding Behavior, And Reticulorumen Environment In Cows And Calves Under Subacute Ruminal Acidosis Risk, Gustavo Mazon Correa Alves

Theses and Dissertations--Animal and Food Sciences

Subacute ruminal acidosis (SARA) is a digestive disorder in ruminants characterized by extended periods of low reticulorumen pH. This digestive disorder is commonly observed in ruminants fed diets with elevated proportions of non-fibrous carbohydrates. This disorder has been vastly studied in adult dairy cattle and has been associated with losses in milk production, changes in feeding behavior, damage to the gastrointestinal tract, and premature culling. Although vastly studied in adult cattle, there is limited research on the effects of SARA in calves. SARA might be prevented by modifying the reticulorumen environment with probiotic supplements containing lactateutilizing bacteria such as Megasphaera …

Effects Of Dietary Supplementation Of A Blend Of Saccharomyces Cerevisiae, Multiple Live Probiotic Bacteria, And Their Fermentation Products On Performance, Health, And Rumen Bacterial Community Of Newly Weaned Beef Steers During A 56-D Receiving Period, Emily Treon

Graduate Theses, Dissertations, and Problem Reports

We examined the effects of a blend of Saccharomyces cerevisiae, multiple live probiotic bacteria and their fermentation products on performance, health, and the ruminal bacterial community of newly weaned beef steers during a 56-day receiving period. Forty newly weaned Angus crossbred steers (221 ± 25.6 kg BW; 180 ± 17 d of age) were stratified by body weight into four pens (10 steers per pen) such that each pen had a similar average body weight at the beginning of the experiment. The pens were randomly assigned to receive a corn silage basal diet (CON; n = 20) or the …

A Road Map For Place Based Collaboration For Conflict Reduction, Joseph L. Zecher

Undergraduate Theses, Professional Papers, and Capstone Artifacts

No abstract provided.

Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl

Electronic Theses and Dissertations

The Afrikaner breed of cattle is indigenous to South Africa and due to their hardiness was once the most popular breed amongst farmers. The objectives for this study were to: (1) estimate environmental effects (age at first calving, season) and (2) genetic variance affecting longevity in Afrikaner cattle using survival analysis and a Weibull model and (3) evaluate the effect of the Infusion Project on longevity utilizing a Cox model. For the first objective, age at first calving impacted longevity as younger age at first calving was associated with decreased longevity. Season of birth and year of birth also impacted …

Valuation Of Soybean Meal Versus Distillers Co-Products As A Protein Supplement For Finishing Beef Cattle: Effects On Growth Performance, Carcass Characteristics, And Efficiency Of Dietary Net Energy Utilization, Cassidy R. Ross

Electronic Theses and Dissertations

Two randomized complete block design experiments were conducted to: 1) examine the effects of soybean meal with or without additional soybean hulls in replacement of modified corn distillers grains plus (MDGS) solubles on growth performance efficiency of dietary net energy utilization and carcass traits responses in finishing beef steers, 2) determine if partial or complete replacement of dried distillers grains plus solubles (DDGS) with solvent-extracted soybean meal (SBM) in diets based upon high-moisture ensiled corn influences growth performance, efficiency of dietary net energy utilization, sera urea-N (SUN) concentrations, or carcass traits in finishing beef steers. Experiment 1 was a 118-d …

Paternal Programming: The Role Of Seminal Plasma In Pregnancy Hemodynamics And Offspring Growth, Rebecca Michele Swanson

Theses and Dissertations

Seminal plasma is commonly known to serve as a transport medium for sperm as it moves through the female reproductive tract for fertilization, however, more recent evidence demonstrates seminal plasma induces an expansive inflammatory response in the uterus. Murine models have found this inflammatory response is important for clearing pathogens and poor-quality sperm, eliciting the secretion of cytokines, chemokines, and embryokines that aid in embryo attachment and growth, placental angiogenesis, and blunting maternal immunity to the embryo. However, there is minimal research on the impacts of seminal plasma uterine priming in bovine, and more specifically embryo growth, uterine blood flow, …

Effects Of Supplementing Sources Of Zinc On Digestibility Parameters Of Beef Steers, Arminda Spikes James

Theses and Dissertations

Zinc (Zn) has numerous functions and life sustaining processes depend on the presence of Zn within the body. Zinc sulfate is a common form of Zn supplemented in the beef industry. Inorganic sulfate based minerals have been associated with negative rumen effects, mainly, decreased rumen fermentation and protozoa numbers. Our studies concluded no difference in digestibility of three different forms of Zn (zinc glycinate, zinc sulfate, and zinc hydroxychloride). Although overall digestibility did not change, there were specific mineral concentration differences after steers consumed concentrate treatment for 10 days. These differences implied that the mineral fed was consumed and absorbed …

Evaluation Of Forage Nutritive Value And Dry Matter Yield Of Stockpiled Tall Fescue Across Plant Hardiness Zones 7 And 8, Malerie Elizabeth Fancher

Masters Theses

Pastures in Tennessee comprise mainly tall fescue [Schedonorus arundinaceus (Schreb.) Dumort.], a cool-season perennial grass. Tennessee producers feed hay for an average of 143 days per year, there is interest in utilizing stockpiling to extend the grazing season. Although this practice has been proven to reduce hay needs (Freeman et al., 2019), it is vital to maintain up-to-date recommendations using data from across the southeastern US to inform nutritional management decisions. The objective of this study is to determine the nutritive value and dry matter yield of stockpiled tall fescue across plant hardiness zones 7 and 8. Fourteen farms …

The Effects Of Sous Vide Cooking And Cysteine Proteases On Instrumental And Sensory Textural Properties Of Lower Value Cuts Of Beef, Ian Smith

All Theses

Sous vide cooking has the potential to increase the value of lower quality cuts of beef. Understanding the textural properties following a cook-chill sous vide process is crucial to maximizing this potential. The use of enzymes may further increase the value of these products if able to positively alter the texture of the final product.

The first research objective was to use instrumental texture analysis to evaluate potential relationships between enzymatic treatments and sous vide processing time of lower value cuts of beef. Three cuts were evaluated: infraspinatus (top blade), semitendinosus (eye of round), and beef tongue. Infraspinatus and semitendinosus …

Evaluating The Role Of The Bovine Vaginal Microbiome In Neonatal And Maternal Health Outcomes, Riley D. Messman

Theses and Dissertations

The dam vaginal microbiota is the first major microbial inoculating community within the neonate. The composition of the dam vaginal microbiota has implications in calf commensal microbiota development. Alterations of the dam microbial community prior to parturition could alter inoculating communities and immune responses in both the dam and calf. Thus, authors aimed to elucidate the microbial community composition of the bovine dam vaginal and calf nasal microbiota post-partum after utilizing betadine lavages (BL). The dam vaginal and calf nasal microbial communities and immune responses were evaluated at 0-, 15-, 30- and 60-day post-partum. Microbiota composition of the dam haircoat, …

Effects Of Supplemental Trace Minerals As Amino Acid Complexed Or Inorganic Sources For Beef Cattle From Receiving Through Finishing, Robin Ashley Cheek

Graduate Theses and Dissertations

The objective of experiments was to investigate the effects of inorganic or amino acid-complexed sources of trace minerals on health, gain performance, liver mineral concentrations, and carcass characteristics of beef heifers from receiving through finishing. To investigate the effects of inorganic or amino acid-complexed sources of trace minerals (zinc, copper, manganese, and cobalt) on performance and morbidity of beef heifers during the receiving period, crossbred beef heifer calves arriving on 3 delivery dates were used in a 42-day receiving trial. Treatments consisted of supplemental zinc (360 mg/d), copper (125 mg/d), manganese (200 mg/d), and cobalt (12 mg/d) from complexed (Availa-4, …

Using Novel Phenotypes To Understand And Predict Fertility In Beef Cows, Cassidy Catrett

Masters Theses

Reproduction plays a major role in the production efficiency of livestock species. However, cow-centric reproductive traits are lowly heritable and are not expressed until later in an animal’s lifetime, making phenotypic selection ineffective at generating genetic gain. We can generate genetic progress by focusing selection on the genetic component of reproductive traits using Expected Progeny Differences (EPDs). We used the American Simmental Association’s performance and Total Herd Enrollment data, made up of 533,155 calving records from 303,158 females (132,403 cows and 170,755 heifers), 33,732 of which are genotyped, to develop two continuous and two discrete phenotypes focused on quantifying early …

Pregnancy Influences On The Rumen Environment Of Angus Heifers Differing In Feed Efficiency, Miranda Gabrielle Martin

Masters Theses

With an expected increase in population by 2050, the demand for more animal protein will increase. To improve the sustainability of US cattle production, producers and researchers have historically focused on improving the feed efficiency of steers, producing a more marketable beef product. However, without a successful pregnancy, there would be no marketable animal to feed. The objective of this study was to examine the physiological changes that occur during pregnancy and their impact on the rumen microbial communities and the correlation to feed efficiency status in Angus heifers. Utilizing 17 cannulated Angus heifers, feed efficiency data was previously collected …

Evaluation Of A Nutritional Calming Supplement For Stress Management In Beef Cattle, Randall Mallette

LSU Master's Theses

Two studies were conducted to determine the efficacy of two formulations of a nutritional calming product, Placid 1.0 and Placid 2.0, at keeping beef cattle in a calm state when exposed to environmental factors that put them into an excited state. In the pilot study, nine crossbred beef heifers were orally drenched daily for three days with 60 mL of their respective treatments, pen scores and exit scores were recorded, and blood was collected for cortisol analysis. There were no treatment effects for either treatment on any of the measured behavioral parameters or serum cortisol concentrations.

The products were …

Evaluation Of The Relationship Between Hair Coat Shedding Ability, Apparent Forage Digestibility, And Mineral Status In Angus Cattle, Margaret P. Loftin

Theses and Dissertations

The objective of this study was to evaluate the relationship between hair coat shedding ability, apparent forage digestibility, and mineral status in Angus cattle. Fecal samples were collected on fall calving Angus cows for one week in March, May, and July from 2019-2022. Liver and plasma samples were collected in March and July each year. Fecal samples were analyzed for DM, ash, NDF, and ADF while liver and plasma were analyzed for mineral concentration. Data were analyzed using MIXED procedure of SAS with significance declared at P0.05. Interactions were detected for DM, NDF, ADF, and liver Fe concentration. The model …

Evaluation Of Nutrient Digestibility Of Weaned Calves From Early And Late Shedding Dams, Jennifer Keele

Theses and Dissertations

Researchers have investigated several factors that could alter fetal growth, including nutrient restriction (Valiente et al., 2021), hair shedding (Gray et al., 2011), and extreme hot and cold temperatures (Davidson et al., 2022). Hot temperatures and increased humidity percentages in the southeast United States caused researchers to investigate the hair coats of Angus cattle in the commercial production setting. An improvement in fiber digestibility and calf birth and weaning weights has been observed in Angus dams that shed 50% of the winter hair coat by May (Gray et al., 2011; Burnett et al., 2021). Our objective of this experiment was …

Identifying Barriers To Data Use On U.S. Beef Cow-Calf Operations And Developing Solutions To Improve Cow-Calf Record-Keeping, William Isaac Jumper

Theses and Dissertations

Cattle health and production records (CHPR) are data collected by cattle producers and veterinarians in the form of measurements, observations, counts of events over time, and physiologic attributes that describe individual and group-level health and production. These data are useful to both veterinarians and cattle producers for making evidence-based decisions on cow-calf operations. Currently, there are no uniform, industry-wide methods of capturing and recording CHPR in the U.S. cow-calf industry. Although many cow-calf producers in the U.S. are thought to collect some form of CHPR, it is believed that relatively few are doing so in an electronic manner that facilitates …

Effect Of Soy Co-Products In Supplements For Grazing Cattle On Growth, Complete Blood Cell Counts, And Physiological Response Following A Lipopolysaccharide Challenge, Bralee Lansdell

Biological Sciences Undergraduate Honors Theses

The objective of this study was to determine the effect of soy co-products (soybean meal and soy oil) in the diet on the growth and hemocytology of cattle during a 56-day growing phase and the physiological/behavioral response to an endotoxin challenge. Angus crossbred steers (n = 36; 289 ± 31 kg, initial body weight ± SD) were stratified by body weight and sire; and assigned randomly to pastures (n = 9; 0.45 ha/mixed-grass pasture). Pastures were assigned randomly to 1 of 3 dietary treatments: 1) a control supplement containing no soy co-products, 2) a supplement containing soybean meal, or 3) …

Influence Of Pregnant Cattle Grazing Endophyte-Infected Tall Fescue On Offspring Immune Function, Sidney Nicole Dunkel

Graduate Theses and Dissertations

The objective was to evaluate the influence of pregnant cattle grazing endophyte-infected tall, toxic, fescue (E+) during gestation on innate immune response of male offspring to an endotoxin challenge. Crossbred multiparous cows (n = 36) were bred to Red Angus sires and housed in replicated E+ or novel endophyte-infected, non-toxic, fescue (NE+) pastures prior to breeding and throughout gestation. From this calf-crop, a subset of post-weaned steers (n = 8 E+; n = 8 NE+) were selected for a lipopolysaccharide (LPS) challenge. Blood was collected every 30 min beginning 2 h prior to (Pre-LPS) and continuing 9 h following (Post-LPS) …

Pharmacokinetics Of Single Feeding Of Cannabidiol In Cattle: A Pilot Study, Haley Cornette

Honors College Theses

Cannabidiol (CBD) is a substance that has been used in complementary medicine for many years. However, modern medicine has little knowledge of how this substance is utilized and metabolized in ruminant animals. Regulations on quality assurance and use in animals are lacking, and CBD supplementation in livestock is not approved. If CBD supplements can be shown to be safe and effective, detection in the animal will be important for determining regulation of use. A withdrawal period can then be established to allow time for deterioration of product to safe levels before livestock products enter human markets. This study sought to …

Increasing Efficiency Of Finishing Beef Production: Strategies To Optimize Starch Digestion, Improve Flake Quality, And Reduce Methane Emissions, Ronald J. Trotta

Theses and Dissertations--Animal and Food Sciences

To continue to meet the demands of increasing food production and environmental sustainability, new strategies are needed to enhance the efficiency of finishing cattle production. A review of the literature identified several nutritional and physiological constraints that limit the ability for cattle to digest starch and absorb glucose from the small intestine. Comparative sequencing analyses revealed a missing region of the sucrase-isomaltase protein which could affect the capacity for starch digestion in cattle. Post-flaking sampling and handling recommendations were generated based on a series of experiments to improve estimates of starch availability and flaking consistency for commercial feedlots and laboratories. …

Selenium Form-Induced Changes In The Early Luteal Phase Corpus Luteum, The Blood, And The Endometrium During Early Gestation In Beef Cows, Sarah Nancy Carr

Theses and Dissertations--Animal and Food Sciences

Selenium (Se) deficient soils and thus forages are widespread throughout the United States, necessitating supplementation of this trace mineral in the diet of grass-fed cattle to negate detrimental effects of a deficiency on immune function, growth, and fertility. Conventionally, Se is supplemented to cattle in a vitamin-mineral mix as an inorganic form (ISe) although the organic forms (OSe) are available when cattle consumer forage, and OSe has been shown to increase the bioavailability of Se in cattle. Previous research has demonstrated supplementation with 1:1 mixture (MIX) of ISe and OSe compared to ISe alone increases systemic concentrations of progesterone (P4) …

The Prevalence And Impact Of Dairy/Beef Crosses On Cattle Producers In New Hampshire, Eleanor J. Braun

Honors Theses and Capstones

Recent years had seen a significant rise in the use of beef semen on dairy cows in the United States. This trend was not widely understood, especially as it related to dairy farmers in New Hampshire. A survey was sent out by mail to the ninety-seven dairy farms in New Hampshire. There were thirty-seven responses to the survey. Thirty-two out of thirty-seven respondents bred beef semen to their dairy cows. Twenty-one of these respondents raised dairy x beef on their farms and twenty-six planned to continue breeding dairy x beef. The most common breeds used in dairy x beef crosses …

Profiling The Cell Wall Polysaccharides Of Hemp (Cannabis Sativa L.) Inflorescence And Hempseed And Exploring Their Application As Ruminant Feed Ingredients, Miranda Agbana

Theses and Dissertations--Animal and Food Sciences

Hemp (Cannabis sativa L.) is used in the United States as a source of CBD oil, fiber, and grain. While hempseeds are consumed whole or as dehulled hemp hearts, hempseed oil is also a desirable seed oil in human nutrition. The process of collecting hempseed oil leaves behind hempseed cakes that are abundant in protein and fiber. Feeding of hempseed cakes to animals, including ruminants, has only initially been explored. Extraction of CBD from hemp inflorescences generates organic waste that may find a second purpose as a fiber feed for cattle. Little is known about the exact fiber composition …

The Investigation Of Novel Bovine Oocyte-Specific Long Non-Coding Rnas And Their Roles In Oocyte Maturation And Early Embryonic Development, Jaelyn Zoe Current

Graduate Theses, Dissertations, and Problem Reports

Early embryonic loss is a significant factor in livestock species' infertility, resulting in an economic deficit. In cattle, the in vivo fertilization rate is ~90%, with an average calving rate of about 55%, indicating an embryonic-fetal mortality rate of roughly 35%. Further, 70-80% of total embryonic loss in cattle occurs during the first three weeks after insemination, particularly between days 7-16. Growing evidence indicates that the oocyte plays an active role in regulating critical aspects of the reproductive process required for successful fertilization, embryo development, and pregnancy. However, defining oocyte quality remains enigmatic. Recently, many have abandoned the notion that …

Potentials Of Pleurotus: Reimagining The Relationship Between Cattle And Brewer's Spent Grain, Zoe Stojkovic

Senior Projects Spring 2023

Brewer’s spent grains (BSGs), a byproduct from beer production, are generated in excess globally. Most often, they are sold or given to proximate cattle farmers for use as feed. However, spent grain can also be used as a medium for fungal cultivation. Given that certain fungal species have the capability to degrade lignin and produce protein, the cultivation of fungi on spent grains may serve to enhance the nutritional profile of the grains for their use as cattle feed. This project is an effort to determine the compatibility of fungal cultivation with BSGs in order to both improve upon cattle …

Rumen And Urine Amine/Phenol-Metabolome Of Beef Steers With Divergent Residual Feed Intake, Taylor S. Sidney

Graduate Theses, Dissertations, and Problem Reports

Cattle production is one of the most important agricultural industries in the United States. Despite its overall production efficiency, the industry is facing considerable adversities to its long-term sustainability due to population growth, decreased land availability, and the increase in global warming. Since feed efficiency imposes an immense influence on the cost of production for animals and is a major cost determinant for profitability, the improvement of feed utilization through the understanding of animal-to-animal variation in feed efficiency is pertinent to increase farm productivity and profitability. Residual feed intake (RFI) is a measure of feed efficiency and is defined as …

Exploring The Biological Basis Of Residual Feed Intake In Beef Cattle Using Multi-Omics Analysis., Godstime Ayobami Taiwo

Graduate Theses, Dissertations, and Problem Reports

Efficient feed utilization is critical for both economic sustainability and environmental responsibility in modern livestock production. While there has been extensive research, the multifaceted nature of feed efficiency remains complex, with many biological factors still unexplored. This dissertation examines the physiological foundations of feed efficiency by elucidating some of the complex biological mechanisms associated with residual feed intake (RFI) phenotype in beef cattle, using a range of Omics approaches. We hypothesized that metabolites related to amino acids, carbohydrates, and fatty acids could act as potential biomarkers for RFI. Through a chemical group-based metabolomics method, we identified enriched pathways in feed-efficient …

Effect Of Lighter (273 Kg) And Heavier (356 Kg) Initial Weight On Growth Performance Of Single-Source, Pre-Conditioned Beef Steers Fed A Single Growing-Finishing Diet, Thomas C. Norman

Electronic Theses and Dissertations

The objective of the study was to determine the influence that initial BW has on growth performance responses, efficiency of dietary net energy (NE) utilization, and carcass traits in feedlot steers. Light- and heavy-weight Charolais×Red Angus steers (n = 70) selected from a larger single-source group were used in a 209-d growing-finishing feedlot experiment at the Ruminant Nutrition Center, in Brookings, SD. On d-0 and d-1 weight and hip height (HH) measurements were collected for allotment purposes; the initial experimental weight was the average between d 0 and d 1 BW. Steers were assigned to two groups based on initial …