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Bacteriology

South Dakota State University

Electronic Theses and Dissertations

2022

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Full-Text Articles in Life Sciences

E. Coli Persister Cell Survival And Rhizobia Attachment To Soybean Roots, Tanim Islam Jan 2022

E. Coli Persister Cell Survival And Rhizobia Attachment To Soybean Roots, Tanim Islam

Electronic Theses and Dissertations

The theme of this thesis revolves around how bacteria respond and thrive during stress. Chapters 1-3 are about how bacteria deal with life-threatening antibiotics. Chapter 4 covers new research on how bacteria can move from a stressful individual lifestyle (free-living bacteria) to initiating a symbiotic relationship with a plant (a less stressful lifestyle). In Chapter 1, I briefly summarize the current state of knowledge in the field of antibiotic resistance and persistence. In Chapter 2, I add to this knowledge by providing new insights into several antibiotics' potency and exploring the antibiotic Eagle effect. In Chapter 3, I use pyruvate …


Characterization Of Porcine Respiratory Epithelial Cells And Their Innate Immune Responses To Bacterial And Viral Ligands, Yam Prasad Gautam Jan 2022

Characterization Of Porcine Respiratory Epithelial Cells And Their Innate Immune Responses To Bacterial And Viral Ligands, Yam Prasad Gautam

Electronic Theses and Dissertations

In response to a pathogenic attack, the host produces a series of defense mechanisms through various intracellular signaling pathways. The byproduct of these signaling pathways helps tackle the invading pathogen and protects the body from getting into a diseased state. This system is called the immune system. The immune system can be divided into two branches namely the innate immune system and adaptive immune system. The groups of immune cells that provide protection regardless of the pathogen specificity constitute the innate immune system. The system that acts according to the pathogen specificity is called the adaptive immune response. The production …


Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah Jan 2022

Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah

Electronic Theses and Dissertations

Plant-based diets contain numerous flavonoid compounds that produce antibacterial effects and gut health improvement. Genistein is among the most abundant isoflavones present in a plant-based diet and can be found in high amounts in soy products (up to 1g Kg-1). We describe here a robust method to identify genistein tolerant and metabolizing bacteria in swine gut microbiota and to screen the strains that have antibacterial and immunestimulatory properties. Such strains could be developed as non-antibiotic alternatives to prevent enteric infections in pigs and improve gut immunity. To this end, a mini bioreactorbased system was used to enrich genistein metabolizing bacteria …