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Full-Text Articles in Life Sciences
Investigating Antimicrobial Properties Of Snake Venoms Against B. Cereus, B. Subtilis, E. Coli, And P. Vulgaris, Savannah Berger
Investigating Antimicrobial Properties Of Snake Venoms Against B. Cereus, B. Subtilis, E. Coli, And P. Vulgaris, Savannah Berger
Honors Program Theses and Research Projects
The increasing incidence of antibiotic resistant bacterial infections has provoked the attention of health officials and scientists as a major threat to global public health. Antibiotic resistance is the product of overprescription by doctors, ineffective or shortened dosing by the patient, and more. When a bacterial infection is ineffectively treated as such, persistent pathogenic cells are given the opportunity to proliferate and spread their resistance to other cells. With bacteria utilizing such tools to fight and ultimately resist our current treatment methods, investigation towards the next novel mechanism of inhibition is essential. Venom is of particular interest to many scientists …
Regulation, Mechanism Of Action, And Function Of A Small Toxin Protein In Ehec, Bikash Bogati
Regulation, Mechanism Of Action, And Function Of A Small Toxin Protein In Ehec, Bikash Bogati
Doctoral Dissertations
The zor-orz locus identified in the chromosome of Escherichia coli O157:H7 EDL933 is a type I toxin-antitoxin system that consists of two homologous gene pairs zorO-orzO and zorP-orzP. The zor genes encode for small toxin proteins and the orz genes encode for small RNAs. Previously it was shown that overproduction of ZorO results in cell growth stasis whereas co-expression of orzO rescues cellular growth. Within, I demonstrate that in addition to growth inhibition, ZorO overproduction results in membrane depolarization and ATP depletion but does not impact the gross morphology of E. coli. In vivo translation and subsequent impacts …
Assesment Of Antibiotic Resistant Gene Expression In Clinical Isolates Of Pseudomonas Aeruginosa, Dustin Esmond
Assesment Of Antibiotic Resistant Gene Expression In Clinical Isolates Of Pseudomonas Aeruginosa, Dustin Esmond
Biology Theses
Increasing prevalence of nosocomial infections by antimicrobial resistant pathogens resulting in higher mortality rates and financial burden is of great concern. Pseudomonas aeruginosa represents one of six highly virulent “ESKAPE” pathogens that exhibit considerable intrinsic drug resistance as well as mechanisms for acquiring further resistance. As many of these mechanisms are regulated through gene expression, we sought to identify regulatory strategies and patterns at play in 23 clinical isolates collected from Baku, Azerbaijan and Tyler, Texas, USA. Real-time quantitative polymerase chain reaction was performed on six gene targets implicated in resistance and contrasted with antibiotic phenotypes. We found AmpC cephalosporinase …
The Role Of The Msaabcr Operon In Cell Wall Integrity And Programmed Cell Death During Biofilm Development, Bibek G C
Dissertations
Staphylococcus aureus is an important human pathogen in both community and health care settings. Biggest challenges with S. aureus as a pathogen is its ability to acquire antibiotic resistance and produce robust biofilms. In this work, we investigated the nature of the cell wall defect in the msaABCR operon mutant in the Mu50 (VISA) and USA300 LAC methicillin-resistant Staphylococcus aureus (MRSA) strains. Results showed that msaABCR-mutant cells had decreased cell wall thickness and cell wall crosslinking in both strains. These defects are most likely due to increased murein hydrolase activity and/or nonspecific processing of murein hydrolases mediated by increased …
Characterizing The Diversity Of Cluster R Prophage In Mycobacterium Abscessus, Colin Welch
Characterizing The Diversity Of Cluster R Prophage In Mycobacterium Abscessus, Colin Welch
Honors College
Mycobacterium abscessus is a mycobacterial pathogen responsible for pulmonary and disseminated infections in susceptible individuals and often is resistant to all antibiotic therapies, with cure rates ranging from 25% to 58% (Degiacomi et al., 2019).Investigating the mechanisms of extensive resistance in M. abscessus lends opportunities to develop more effective treatments. Prophage, viral sequences integrated into bacterial genomes, contribute to virulence and fitness in many bacterial pathogens including Escherichia coli O157:H7 and Vibrio cholerae (Fortier & Sekulovic, 2013). Yet, prophage in pathogenic mycobacteria are not well understood or studied. Recent work has demonstrated that the Mycobacterium chelonae prophage, McProf can function …