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Full-Text Articles in Life Sciences
Rapid Detection Of Listeria Monocytogenes, Wim Lippens
Rapid Detection Of Listeria Monocytogenes, Wim Lippens
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Listeria monocytogenes is a foodbome pathogen that can cause severe illness and even death. It is found in dairy and meat products. The focus is on rapid detection since conventional methods are time consuming (4-5 days). Pre-enrichment steps, as part of those methods, are time consuming. Our objective was to develop a detection system without a pre-enrichment step, giving a final result within 2 to 4 h.
In the concept of "the need for speed," a detection system with an antibody-based capture technique, followed by polymerase chain reaction (PCR), was developed. Glass beads coated with a Listeria polyclonal antibody were …
Production Of Volatile Sulfur Compounds From Inorganic Sulfur By Lactococci, Supriyo Ghosh
Production Of Volatile Sulfur Compounds From Inorganic Sulfur By Lactococci, Supriyo Ghosh
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Production of volatile sulfur compounds in cheese is associated with desirable flavors. The direct source of these compounds has been assumed to arise from the metabolism of methionine and cysteine. However, the methionine concentration in cheese rises above the amount found in casein during aging, suggesting that alternative sulfur sources are present in milk. This led us to hypothesize that lactococci may acquire sulfur from the inorganic sulfur pool of milk, in addition to methionine and cysteine, to generate volatile sulfur compounds during cheese ripening.
A turbidimetric method to determine total sulfate content in milk samples was developed. The average …