Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Characterization Of Transcriptional Control Elements In Cluster E Mycobacteriophage Ukulele, Campbell Belisle Haley
Characterization Of Transcriptional Control Elements In Cluster E Mycobacteriophage Ukulele, Campbell Belisle Haley
Honors College
Mycobacteriophage (phage) are a diverse group of viruses that infect Mycobacterium. Their study allows further understanding of viral evolution and genetics. Phage tightly control gene expression and transcribe their genes using host RNA polymerases. This project identifies potential transcriptional control elements in the genome of mycobacteriophage Ukulele. Promoters are sequences of the genome that allow binding of RNA polymerase and initiation of transcription. 21 putative promoters were identified in the Ukulele genome. To confirm transcriptional activity from putative promoters, a GFP reporter system was developed in mycobacterial cells. Intrinsic terminators are mRNA sequences that form secondary structure during transcription …