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Multiple Hemoglobins In Rainbow Trout, Salmo Gairdneri, And Cutthroat Trout, Salmo Clarkii, Jeffrey Carl Braman
Multiple Hemoglobins In Rainbow Trout, Salmo Gairdneri, And Cutthroat Trout, Salmo Clarkii, Jeffrey Carl Braman
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Nine hemoglobins from adult rainbow trout have been isolated by starch gel electrophoresis using a Trisborate buffer system, pH 8.7. Six fast and two slow anodally migrating hemoglobins, and one slow cathodally migrating hemoglobin were observed in all specimens.
The nine hemoglobins have been purified by DEAE cellulose ion exchange chromatography followed by starch gel electrophoresis.
The subunit structure of each purified hemoglobin has been partially examined by gel electrofocusing (GEF) in .5 percent polyacrylamide gels containing 8M urea and lmM dithiothereitol (DTT). Tetrameric combinations of one, two, three, and four polypeptide chains are present.
Twelve hemoglobins have been isolated …