Life Sciences Commons^™

Multiple Hemoglobins In Rainbow Trout, Salmo Gairdneri, And Cutthroat Trout, Salmo Clarkii, Jeffrey Carl Braman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nine hemoglobins from adult rainbow trout have been isolated by starch gel electrophoresis using a Trisborate buffer system, pH 8.7. Six fast and two slow anodally migrating hemoglobins, and one slow cathodally migrating hemoglobin were observed in all specimens.

The nine hemoglobins have been purified by DEAE cellulose ion exchange chromatography followed by starch gel electrophoresis.

The subunit structure of each purified hemoglobin has been partially examined by gel electrofocusing (GEF) in .5 percent polyacrylamide gels containing 8M urea and lmM dithiothereitol (DTT). Tetrameric combinations of one, two, three, and four polypeptide chains are present.

Twelve hemoglobins have been isolated …

Laboratory Apparatus For Providing Diel Temperature Regimes For Aquatic Animals, Wayne A. Wurtsbaugh, G. Davis

Watershed Sciences Faculty Publications

In studies of the effects of elevated temperature on aquatic organisms, little emphasis has been placed on modeling the fluctuating temperatures to which organisms are exposed in nature. To some extent this may be related to problems of design and maintenance of apparatus for fluctuating temperature control. The complexity of apparatus used to provide cycled temperatures has ranged from manually controlled valves for introducing water of different temperatures into aquariums (Kelso 1972), to a complex and expensive feedback system, regulated by rotating cams, that controls the temperature of influent water (Honeywell Cam Programmer Thermometers, Fort Washington, Pennsylvania). To facilitate studies …