Life Sciences Commons^™

An Intersection Of Science & Art: Vitrification Approaches And Open-Fabricated Tools For The Biomedical Model Sea Hare, Aplysia Californica, Allyssa M. Oune

LSU Master's Theses

The California sea hare (Aplysia californica) is an important biomedical model for molecular neurobiology, electrophysiology, learning, and memory due to their well-mapped and large neurons and well-characterized learning capabilities. The National Resource for Aplysia (NRA, University of Miami) maintains large stocks of live animals and relies on regular shipments of wild-caught individuals to maintain genetic diversity. This is labor and cost-intensive, and environmental changes could alter the availability of wild animals increasing the need to preserve this genetic resource. One solution is vitrification, ultra-fast cooling which produces an amorphous glass that minimizes damage to cells. Aplysia californica presents …

Do Fishery Meeting Attendees Represent The Fishing Industries?, Dominique Seibert

LSU Master's Theses

Public meetings are a highly utilized tool for disseminating important or useful information. Many agencies rely on them to reach various stakeholders and community representation at these meetings is important. A substantial amount of research has been conducted on various aspects of public meetings including different techniques, factors impacting attendance, and representativeness, that is attendees’ opinions representing the opinions at-large. A noticeable gap in the research includes information on public fishery meetings, commercial fishing industries, and their members’ participation. With the U.S. commercial fishing industries supporting more than a million jobs and providing more than a 200-billion-dollar economic impact annually, …

Evaluating Vaccine Management Strategies For Edwardsiella Ictaluri Infections In Zebrafish (Danio Rerio), Brandy Malbrough

LSU Master's Theses

Aquaculture is a globally important industry that faces significant challenges due to infectious diseases, which can result in substantial financial losses. In 2011, a new strain of Edwardsiella ictaluri emerged as a major pathogen affecting zebrafish in the ornamental fish industry, leading to high mortality rates and posing a severe threat to the sector. Vaccines provide potential benefits for disease prevention in aquaculture, particularly live-attenuated vaccines (LAVs), which induce strong host immune responses. Bacterial attenuation for vaccine development has evolved from serial passage to more targeted genetic modification techniques. However, regulatory constraints limit the availability of licensed LAVs for aquaculture. …

Integrating Germplasm Repositories Into Oyster Aquaculture Systems At The Pathway, Center, And Network Levels, Sarah R. Bodenstein

LSU Doctoral Dissertations

Germplasm repositories offer many potential benefits to commercial farm operations, including preserving genetic resources and increasing production capabilities. Aquaculture industries have yet to take advantage of repository storage despite decades of research evaluating cryopreservation protocols. Eastern oysters (Crassostrea virginica), for example, are a useful research species when addressing this problem. Oysters are an economically important aquaculture species and a high-throughput cryopreservation method for oysters already exists. To address the gap between protocol research and commercial application a different approach is necessary that can analyze the requirements of repository development at multiple organizational levels. The goal of this dissertation was to …

Gulf Sturgeon Mesohabitat Use And Movement In The Lower Pearl River Louisiana-Mississippi, Amanda Popovich

LSU Master's Theses

After spring migrations to natal rivers Gulf Sturgeon Acipenser oxyrinchus desotoi reside in freshwater holding areas prior to fall emigrations to nearby estuaries. Limited published information on the physical habitat characteristics of these holding areas is available, especially for juvenile fish in the western portion of the Gulf Sturgeon’s distribution. To further investigate freshwater occupancy and movement patterns, 32 acoustic receivers were deployed from late April to October of 2021 and 2022 in 1600-m reaches distributed throughout the lower Pearl River, Louisiana-Mississippi, the westernmost river within their distribution. Generalized linear mixed models and multi-state models were used to determine what …

Fish And Invertebrate Community And Trophic Structure In The Gulf Of Mexico Coastal Plains Watersheds Across Varying Hydrologic Connectivity., Erin Elizabeth Thayer

LSU Doctoral Dissertations

Subtropical Northern Gulf of Mexico coastal plains watersheds tend to have mild seasonal temperature, low topography, and fine, mostly homogenous substrate sizes; these watersheds may not follow fish and invertebrate community patterns predicted by established lotic paradigms because those paradigms tend to describe communities in either temperate or tropical regions. However, the bases of most generally accepted lotic paradigms involve evaluation of allochthonous and autochthonous contributions to lotic ecosystems via three hydrologic connectivity dimensions including vertical (hyporheic and shallow groundwater), lateral (floodplain), and longitudinal (upstream and downstream). My dissertation goals were to explore these three dimensions within Louisiana coastal plains …

Characterizing Habitat Suitability For Gulf Sturgeon (Acipenser Oxyrinchus Desotoi) In Southern Louisiana, Jenna N. Brogdon

LSU Master's Theses

A generalized additive modeling (GAM) framework was used to characterize fish-habitat relationships and develop habitat suitability maps to predict spatiotemporal and ontogenetic shifts in Gulf sturgeon distribution within an impacted estuary in the northern Gulf of Mexico, Lake Pontchartrain. Gulf sturgeon (n = 103) were fitted with acoustic transmitters in the Pearl River during the early summer and fall from 2013 to 2018, and an array of acoustic receivers (n = 81) was used to monitor Gulf sturgeon habitat use and movement in the estuary from 2016 to 2019. Daily presence data from the telemetry array were paired with environmental …

A Modular Open-Technology Device To Measure And Adjust Concentration Of Sperm Samples For Cryopreservation, Nikolas C. Zuchowicz

LSU Master's Theses

Repositories for aquatic germplasm can safeguard the genetic diversity of species of interest to aquaculture, research, and conservation. The development of such repositories is impeded by a lack of standardization both within laboratories and across the research community. Protocols for cryopreservation are often developed ad hoc and without close attention to variables, such as sperm concentration, that strongly affect the success and consistency of cryopreservation. The wide dissemination and use of specialized tools and devices can improve processing reliability, provide data logging, produce custom hardware to address unique problems, and save costs, time, and labor. The goal of the present …

Gain-Of-Function Polymorphisms In Human Inflammasomes: Implications For Cystic Fibrosis, Duane Jeansonne, Samithamby Jeyaseelan

Faculty Publications

No abstract provided.

Effects Of Salinity On Eastern Oysters: Locating Lower-Salinity Tolerant Populations And Defining Resource Zones Suitable To Restoration, Fisheries, And Aquaculture., Lauren Swam

LSU Master's Theses

Eastern oysters (Crassostrea virginica) provide valuable ecosystem services and support a productive commercial industry in the northern Gulf of Mexico. Declining abundance from water quality changes and other factors drives development of management and restoration strategies focused on a comprehensive, metapopulation approach. Identifying oyster resource zones based on water quality combined with selective breeding of oysters adapted to specific conditions provides strategies to support aquaculture development and ensure resilient oyster populations and high production. Using 2015-2019 satellite-derived continuous salinity and temperature data for coastal Louisiana, this work created maps defining oyster resource zones supportive of (1) broodstock sanctuary …

Incorporating Life Into Living Shorelines: Can Gulf Ribbed Mussels Reduce Shoreline Erosion And Enhance Restoration Practices?, Jordan Logarbo

LSU Master's Theses

The gulf ribbed mussel (Geukensia granosissima) exists throughout the Gulf of Mexico and influences biotic and abiotic environmental attributes as an ecosystem engineer. Ribbed mussels are an important component of marsh ecosystems providing services including filtering particulate matter, depositing and transforming nutrients in the system, increasing soil strength via byssal threads and providing structure via their shells.

The spatial distribution of mussels along the marsh edge of Sister Lake, LA was investigated via a broad survey of 150 sites, in relation to elevation, exposure and vegetation percent cover. This survey was followed by a second survey at a …

The Life History And Population Dynamics Of Southern Flounder, Kenneth A. Erickson

LSU Master's Theses

Southern Flounder, Paralichthys lethostigma, are a coastal, estuarine-dependent flatfish species that inhabits the Southeastern US Atlantic Ocean and Gulf of Mexico. Throughout their range, Southern Flounder are exploited by both commercial and recreational fisheries. Recently, numerous sources have expressed concerns about Southern Flounder populations, with stock assessments indicating declines in spawning stock biomass and recruitment. To estimate life history traits needed for informed management, such as age, growth, and maturity, I collected 327 Southern Flounder from Louisiana and obtained 14,184 historical records from the Louisiana Department of Wildlife and Fisheries. Using a von Bertalanffy growth equation in a Bayesian …

Fish Community Composition And Structure Near A Freshwater River Diversion In Southeastern Louisiana, Rachel L. Snider

LSU Master's Theses

Gulf of Mexico estuaries, particularly in Louisiana, are among the world’s most productive, with landings of commercially- and recreationally-important species exceeding all other contiguous US states. Coastal wetlands are disappearing at an alarming rate because they have been impounded from Mississippi River water and sediment. Although controversial, one proposed solution is to re-route the Mississippi River through diversions and siphons to supply the freshwater and sediments necessary to rebuild vanishing wetlands, particularly in Barataria Bay and Breton Sound, LA. This strategy is one approach outlined in the Louisiana Coastal Master Plan. This project aimed to describe the composition and structure …

Role Of Coastal Environmental Conditions During Austral Winter On Plankton Community Dynamics And The Occurrence Of Pseudo-Nitzschia Spp. And Domoic Acid In Inhambane Province, Mozambique, Holly Kelchner

LSU Master's Theses

Harmful algal blooms (HABs) are increasing globally in frequency, persistence, and geographic extent. HABs pose a threat to economic stability, and ecosystem and human health. To date no incidences of marine toxins produced by phytoplankton have been recorded in Mozambique, which may be due to the absence of a monitoring program and general awareness of the potential threat. This study is the first documentation of the occurrence of a neurotoxin, domoic acid (DA), produced by the diatom genus Pseudo-nitzschia spp. along the east coast of Africa. The coast of Inhambane Province is a biodiversity hotspot where year-round Rhincodon typus (whale …

Artificial Intelligence And Covid-19: Deep Learning Approaches For Diagnosis And Treatment, M. B. Jamshidi, A. Lalbakhsh, J. Talla, Z. Peroutka, F. Hadjilooei, P Lalbakhsh, M. Jamshidi, L. La Spada, M. Mirmozafari, M. Dehghani, A. Sabet, Sa. Roshani, So. Roshani, N. Bayat-Makou, B. Mohamadzade, Z. Malek, A. Jamshidi, S. Kiani, H. Hashemi-Dezaki, W. Mohyuddin

Faculty Publications

COVID-19 outbreak has put the whole world in an unprecedented difficult situation bringing life around the world to a frightening halt and claiming thousands of lives. Due to COVID-19 & x2019;s spread in 212 countries and territories and increasing numbers of infected cases and death tolls mounting to 5,212,172 and 334,915 (as of May 22 2020), it remains a real threat to the public health system. This paper renders a response to combat the virus through Artificial Intelligence (AI). Some Deep Learning (DL) methods have been illustrated to reach this goal, including Generative Adversarial Networks (GANs), Extreme Learning Machine (ELM), …

Habitat Associations And Reproduction Of Fishes On The Northwestern Gulf Of Mexico Shelf Edge, Elizabeth Marie Keller

LSU Doctoral Dissertations

Several of the northwestern Gulf of Mexico (GOM) shelf-edge banks provide critical hard bottom habitat for coral and fish communities, supporting a wide diversity of ecologically and economically important species. These sites may be fish aggregation and spawning sites and provide important habitat for fish growth and reproduction. Already designated as habitat areas of particular concern, many of these banks are also under consideration for inclusion in the expansion of the Flower Garden Banks National Marine Sanctuary. This project aimed to gain a more comprehensive understanding of the communities and fish species on shelf-edge banks by way of gonad histology, …

Assessment Of Off-Bottom Oyster (Crassostrea Virginica) Aquaculture Techniques On Biofouling In The Northern Gulf Of Mexico, Ellis Chapman

LSU Master's Theses

Although off-bottom oyster aquaculture is a rising industry in the northern Gulf of Mexico, it is susceptible to biofouling, the accumulation of organisms on industry surfaces. Biofouling creates problems for commercial growers by increasing the costs of labor associated with biofouling management. The most used off-bottom production techniques involve aerial exposure. OysterGro™ 6-slot off-bottom oyster cages were used in this project to test aerial exposure frequency, antifouling coatings and bag position on mortality, growth rates, shell ratios and condition indices on four sites in the northern Gulf of Mexico. Aerial exposure of floating cages was performed in increments of once …

Prevalence And Control Of Salmonella Spp. And Sanitary Indicator Microorganisms In Wild Caught And Farm Raised Catfish (Ictalurus Punctatus), Bilan Costley Jessie

LSU Doctoral Dissertations

Catfish inspections have transitioned from the U.S. Food and Drug Administration (FDA) to the United States Department of Agriculture (USDA). The USDA Food Safety and Inspection Service (FSIS) and Food Emergency Response Network (FERN) consider it important to assess the food safety risk associated with consuming catfish in the United States. Surveillance of farm-raised and wild-caught catfish for pathogens is relevant as a public safety protocol. The purpose of this study was to detect the prevalence of Salmonella spp., identify and examine isolates for antibiotic resistance to clinical treatments, evaluate the effectiveness of antimicrobial chemical treatments against Salmonella enterica, and …

Characterizing Louisiana's Freshwater Commercial Fisheries, Lauren Bonatakis

LSU Master's Theses

As an important contributor to Louisiana’s economy, the commercial freshwater fisheries have been the subject of growing attention in recent years by resource managers. Compared to the marine sector, little is known about these fisheries. Anecdotally, the fisheries appear to be on the brink of collapse. Fewer young fishermen are entering the field, fish buyers and processors are closing, and market prices remain stagnant. Because of this, the fisheries lack the resources needed to perpetuate the success and sustainability for future generations. To better understand this industry, I conducted a two-part study to characterize the commercial freshwater fisheries in Louisiana. …

Transforming Lives In Coastal Communities Through Economic Development: Breeding Crassostrea Virginica For Alternative Oyster Culture, Brian Randall Callam

LSU Doctoral Dissertations

This dissertation helped address the commercialization of triploid Crassostrea virginica production and develop tetraploid C. virginica broodstock in Louisiana by addressing triploid commercialization and tetraploid broodstock development. This was done by: 1) developing regional breeding plans for diploid and tetraploid C. virginica targeted at the Gulf Coastal Region, 2) assessing the effect of chromosome set manipulation on gonadal development in diploid, triploid, and tetraploid C. virginica, 3) quantifying the variation of commercially important hatchery traits in triploid C. virginica larvae from different tetraploid parents, 4) developing an up-to-date remote setting manual for the Gulf Coastal Region for industry use, …

An Evaluation Of Sciaenid Growth In The Gulf Of Mexico, Shane Flinn

LSU Master's Theses

Growth models estimate life history parameters that are used in the management of fisheries stocks. The most commonly used growth model in fisheries is the von Bertalanffy growth model (VBGM), yet it has been shown to provide a poor fit for length-at-age data of some species and other models exist. I reviewed 196 peer-reviewed age and growth studies and 50 NOAA stock assessments to examine temporal trends in the use of growth models and model selection in fisheries. I found that the use of multi-model frameworks has increased since the year 2000 and information theoretic approaches are replacing goodness-of-fit and …

Using Local Fishery Monitoring To Understand Small-Scale Coastal Fisheries In Tanzania, Matthew Robertson

LSU Master's Theses

Nearshore marine fisheries provide the main source of protein for nearly 9 million people in the coastal villages of Tanzania, yet for decades the fisheries have shown signs of overexploitation. These fisheries are small-scale and co-managed by local coastal communities in groups known as Beach Management Units (BMUs). BMUs monitor individual fishing trip data (e.g. gear, vessel, taxa); however, these data have only been analyzed in nationally aggregated statistics and to our knowledge, are not presently used in management decision making. The present thesis aimed to identify the forms of data and information that local fishery monitoring can record about …

Conservation Of Freshwater Live-Bearing Fishes: Development Of Germplasm Repositories For Goodeids, Yue Liu

LSU Doctoral Dissertations

Fishes of the family Goodeidae are considered to be among the most imperiled taxa in the world. The goal of this project was to provide a foundation for development of sperm repositories for goodeids and to use them as a model to assist conservation programs for imperiled freshwater live-bearing fishes. Development of such repositories is challenging because of unusual reproductive characteristics of live-bearing fishes, such as sperm bundles, internal fertilization, and the bearing of live young. Standardized methods were established to evaluate quality-related attributes of sperm bundles. The different features between activation of free and bundled sperm were investigated and …

Crayfish Harvesting Practices In The Southern Atchafalaya River Basin: Quantitative And Qualitative Assessment Of Harvester Techniques And Hydrologic Connectivity Influence On Harvesting Strategies, Ivan A. Vargas-Lopez

LSU Master's Theses

Fisheries produce important impacts around the world through the exploitation of a wide range of species. In Louisiana, crayfish is the most emblematic crustacean and supports a multi-million industry based on pond culture and harvest from natural habitats. Although the economic value (USD) of wild-harvested crayfish has decreased from 10% to 3% of total crayfish value from 2013 to 2015, wild harvested crayfish are highly desired by many consumers and have a strong socio-cultural importance in Louisiana and other Gulf of Mexico coastal regions. This project evaluated harvesting practices by: 1) field observation and mapping of harvest sites in southwestern …

The Impact Of Co2-Related Ocean Acidification On The Molecular Regulation Of Shell Development In The Eastern Oyster (Crassostrea Virginica)., Mackenzie L. Richards

LSU Master's Theses

Eastern oysters (Crassostrea virginica), native to the Gulf of Mexico, are keystone species in estuarine ecosystems and are economically valued. Current research indicates that ocean acidification adversely affects the physiology and morphology of larval oysters, but the molecular mechanisms of this impact remain unstudied. Ocean acidification is contributed to by elevated atmospheric CO₂ due to increased anthropogenic activities, causing heightened partial pressure of CO₂ (pCO₂), and eutrophication from land-based runoff in the Gulf. The objective of this work was to determine the genomic response of the eastern oyster in Louisiana to simulated ocean acidification. …

Rice Hull Bioreactor For Recirculating Aquaculture, Marlon A. Greensword

LSU Doctoral Dissertations

The engineering of floating media biofilters has been optimized over the years. The backwashing process has made them more energy and water efficient. Likewise, moving bed bioreactors (MBBR) are gaining interest and popularity because they are relatively affordable to build. Yet, developing countries’ aquaculture production remains largely excluded from the advances made in recirculating aquaculture systems (RAS). This discrepancy is partially driven by the high costs of media such plastic beads and Kaldnes (KMT) media, commonly used in MBBR.

This dissertation evaluates the usability and profitability of rice hulls (RH), an abundant by-product in many developing nations, as a sinking …

Failure Of A Heterologous Recombinant Sca5/Ompb Protein-Based Vaccine To Elicit Effective Protective Immunity Against Rickettsia Rickettsii Infections In C3h/Hen Mice, Sean P. Riley, Marissa M. Cardwell, Yvonne G. Chan, Ludovic Pruneau, Fabio Del Piero, Juan J. Martinez

Faculty Publications

Spotted fever group (SFG) rickettsial species are obligate intracellular tick-borne pathogens that are responsible for important human diseases. Previous reports have demonstrated the feasibility of using recombinant surface cell antigen Sca5/OmpB to elicit protective immunity against homologous challenges using murine models of Mediterranean spotted fever and Rocky Mountain spotted fever. In addition, the feasibility of generating cross-protective immunity against related rickettsial species has also been established, but the molecular basis for these phenomena was not explored. Here, we demonstrate that vaccination of C3H/HeN mice with a recombinant OmpB domain derived from Rickettsia conorii induced high titer humoral immune responses that …

Making A Big Splash With Louisiana Fishes, Prosanta Chakrabarty, Sophie Warny, Valerie Derouen

Special Publications of the Museum of Natural Science

This book is a companion piece to Making a Big Splash with Louisiana Fishes, an exhibit created in 2012 at Louisiana State University’s Museum of Natural Science (MNS)

Response Of The Fruittree Leafroller (Archips Argyrospila) To Salt Tolerant, Baldcypress (Taxodium Distichum) Seedlings, Rachel A. Rollason

Honors Theses

No abstract provided.