Life Sciences Commons^™

Relative Hepatotoxocity, Carcinogenicity, And Toxicogenomics Of Select Dehydropyrrolizidine Alkaloids In Mice, Michael J. Clayton

All Graduate Theses and Dissertations, Fall 2023 to Present

Dehydropyrrolizidine alkaloids are arguably the most important plant derived toxins in terms of impact on human and animal health. Dehydropyrrolizidine alkaloids are a large group of chemically related compounds found in 3% of flowering plants worldwide. Human exposure occurs from ingestion of herbal products including teas supplements or contaminated grain. Animals are exposed through contaminated feed or grazing. There are at least 350 identified toxic PAs, from more than 6,000 plants. The toxins primarily cause liver damage, but some are proven to cause cancer. Indidvidual dehydropyrrolizidine alkaloids vary in their toxic effects. Riddelliine is the only dehydropyrrolizidine alkaloid with extensive …

Heterochronic Serum Proteins, Jeffrey Mason

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Mechanism of ovarian-dependent enhancement of health and longevity in postreproductive female mice

Dynamic Fecal Microbiota Transfer From Mice With Western Diet-Induced Severe Colitis And Colon Tumorigenesis Did Not Exacerbate Colitis Symptoms Or Colon Tumorigenesis In Recipient Mice Fed Either The Ain93g Diet Or The Total Western Diet, Abby D. Benninghoff

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Consumption of the total Western diet (TWD) in mice has been shown to increase gut inflammation, promote colon tumorigenesis, and alter the fecal microbiome composition in mice as com-pared to mice fed a healthy diet, AIN93G (AIN). However, it is unclear whether the gut micro-biome contributes directly to colitis-associated CRC in this model. The objective of this study was to determine whether dynamic fecal microbiota transfer (FMT) from host mice fed either AIN or TWD basal diets would alter colitis symptoms or colitis-associated CRC in recipient mice, which were fed either AIN or TWD directly using a 2x2 factorial experiment …

Effect Of Acute And Repeated Noise Exposure On The Behaviour And Lipid Peroxidation In Brain Tissue Of Male And Female Mice, Gabriella Korsós, Kinga Fodor, Annamária Kiss, Anna Blázovics, Sándor György Fekete

Turkish Journal of Veterinary & Animal Sciences

Despite the increasingly obvious sex differences, male animals are still predominantly used to study stress and related disorders. The aim of this study was to evaluate the effects of repeated noise stress in female and male mice. In the experiment, 12 male and 12 and female SPF/VAF 6-week-old Crl:CD1(lcr) mice were used. After 5 days of acclimatization all animals were placed in the open-field for three consecutive days where each mouse spent 10 min daily without any noise treatment in order to get used to the circumstances of the test. On day 9 to record baseline behaviour the animals were …

Developmental Influences On The Initial Subjective Rewarding Effects Of Etoh, Madison Waldron

Master’s Theses

The present studies were aimed to better understand developmental contributions to the risk for disordered drinking, and facilitate the long-term goal of developing effective interventions for individuals at high risk for alcohol use disorders. Experiment 1 assessed the effect of adolescent pre-exposure to ethanol on adult place preference, as well as, sex- and beta-endorphin(bE)-related contributions. Adolescent C57BL/6J and bE deficient mice were injected with 1.5g/kg of ethanol or saline and put back into their home cages. At the time of adulthood, we employed a single-exposure conditioned place preference paradigm (SE-CPP) to investigate the impact of preexposure on the initial subjective …

Will There Be Changes In Sexually Differentiated Behaviors In Mice Manipulated For The Sry Gene As They Mature Into Adultood?, Subin Joo

CMC Senior Theses

Sexually differentiated behavior has been shown to be affected by both genes and hormones. The discovery of the SRY gene, which codes for the development of testes, lead to the development of the Four Core Genotypes model of mice, and the separation of sex chromosomes and its resulting gonadal hormones. Using the FCG model, this study aims to look at the development of sexually differentiated behavior in mice, and track how it changes throughout their life. FCG mice will be divided into individual and social housing, and repeated experiments carried out to test their reaction to both intact female and …

Tobacco Enhances Bacterial-Induced Periodontal Bone Loss In Mice., Mina Iskander

Electronic Theses and Dissertations

Background: Tobacco smoking is the leading environmental risk factor for periodontal diseases. Delineation of the mechanisms underlying tobacco-induced or exacerbated periodontitis is hampered by the lack of an appropriate and reliable animal model. Hypothesis: We hypothesized that Porphyromonas-gingivalis-infected, cigarette smoke-exposed mice would represent reproducible models of acute (ligature model) and chronic (oral gavage model) tobacco-enhanced periodontitis that reflect multiple aspects of the disease noted in human smokers. Methods: In a chronic oral gavage disease model, Balb/c mice (6-8 weeks, 4 groups of n = 6 per group) were exposed to smoke produced by a Teague-10 smoking machine from 1R6F research …

Characterizing The Range Shifts Of Two Peromyscus Species In Maine, Molly Bennett

Honors College

In a changing climate, two species of mice in Maine (Peromyscus maniculatus and Peromyscus leucopus) are currently undergoing range shifts. The objective of my thesis is to determine the historical and current range of each species within the state of Maine. I used two approaches. I assembled ear biopsies collected this summer in Acadia National Park and throughout the state by the Gardner and Levesque labs to genotype the mice as either P. maniculatus or P. leucopus. Additionally, I summarized research that denotes where the two species were historically present around the state. These species are functionally impossible to tell …

The Effects Of Total Body Proton Irradiation On Mouse Myometrium, Lillith Bulawa

Undergraduate Honors Theses

The boundaries of human space exploration continue to expand with new technology and discoveries making it even more important to investigate the effects of space on biological systems. Although humans have explored space in small increments, reproductive studies must be conducted to determine if stable short- or long-term residences for humans can exist in space. This study explored the effects of whole-body proton radiation on uterine smooth muscle known as the myometrium. Two types of mice utilized in this study were C57BL/6 and B6.129S6Cybbtm1Din/J NOX2 knockout mice. C57BL/6 mice are standard laboratory mice that were used to represent the wildtype …

Granulosa Cell Proliferation Is Inhibited By Pge2 In The Primate Ovulatory Follicle, Patric S. Lundberg, Gil J. Moskowitz, Carmel Bellacose, Esra Demirel, Heidi A. Trau, Diane M. Duffy

Computer Science Faculty Publications

Prostaglandin E2 (PGE2) is a key paracrine mediator of ovulation. Few specific PGE2-regulated gene products have been identified, so we hypothesized that PGE2 may regulate the expression and/or activity of a network of proteins to promote ovulation. To test this concept, Ingenuity Pathway Analysis (IPA) was used to predict PGE2-regulated functionalities in the primate ovulatory follicle. Cynomolgus macaques underwent ovarian stimulation. Follicular granulosa cells were obtained before (0 h) or 36 h after an ovulatory dose of human chorionic gonadotropin (hCG), with ovulation anticipated 37-40 h after hCG. Granulosa cells were obtained from additional monkeys 36 h after treatment with …

Germ Cell-Depleted Ovaries Improve Metabolic Health In Post-Reproductive Mice, Kyleigh Tyler

Research on Capitol Hill

Reproductive senescence is correlated with many deleterious heath conditions. In human females, the decline of health associated with reproductive function is termed “menopause.“ One of the declining health factors is metabolism.

In order to understand how ovaries contribute to metabolic health, we further studied the structure of ovaries. Within ovaries, there are two main types of cells:

• Germ Cells (oocyte): Play a central role in reproduction (egg)
• Somatic Cells: Supporting cells for germ cells

This study aimed to understand the relationship between germ cells and somatic cells and how this relationship influenced metabolism.

Mice And Rats: Perceptions, Realities, And Impacts On Humankind, Terry A. Messmer

Human–Wildlife Interactions

This is the Letter from the Editor.

Small Mammals Of Family Muridae In Protected Areas Of Pakistan, Fakhra Nazir, Andleeb Batool, Inayat Ullah Malik, Safdar Ali Shah, Sabiha Shamim

Journal of Bioresource Management

Murids to have more than 1300 species globally, forming the largest mammal group. Murids are found nearly everywhere in the world, though many subfamilies have narrower ranges. Murids are not found in Antarctica and many oceanic islands. Five National Parks from Northern areas of Pakistan were physically surveyed. The parks were studied at different times. Dhirkot National Park (DNP) in February 2008, Banjosa National Park (BJNP) from May to June 2009, Pir Lasura National Park (PLNP) from June to July 2009 and Pir Chanasi National Park (PCNP) from April to May 2010. A total of 6 species belonging to the …

Targeted Limb Heating Augments The Actions Of Igf1 In The Growth Plate And Increases Bone Elongation In Growing Mice, Holly Lyn Racine

Theses, Dissertations and Capstones

Bone elongation disorders can lead to painful musculoskeletal disabilities in adulthood. Existing treatment options to correct left-right asymmetry in limb length include invasive surgeries and/or drug regimens. These are often only partially effective. Previous studies in weanling mice have shown that a daily application of mild heat (40°C) to limbs on one side of the body could be used to noninvasively enhance bone elongation. However, the impact of heat-treatment on bone at the cellular level remains elusive. The epiphyseal growth plate, the band of cartilage located at each end of long bones, is the main site of longitudinal growth and …

Small-Animal Models Of Zika Virus, Justin G. Julander, Venkatraman Siddharthan

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection can result in serious consequences, including severe congenital manifestations, persistent infection in the testes and neurologic sequelae. After a pandemic emergence, the virus has spread to much of the new world and has been introduced to many countries outside of endemic areas as infected travelers return to their home countries. Rodent models have been important in gaining a better understanding of the wide range of disease etiologies associated with ZIKV infection and for the initial phase of developing countermeasures to prevent or treat viral infections. We discuss herein the advantages and disadvantages of small animal models …

The Protective Efficacy Of Immunoglobulin Y From Immunized Chickens Against Salmonella Infections In Mice, Hasan Hüseyi̇n Hadi̇mli̇, Zafer Sayin, Gökçenur Sani̇oğlu Gölen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the efficacy of immunoglobulin Y (IgY) obtained from chickens immunized with Salmonella vaccines. Chickens were vaccinated three times with inactivated monovalent, bivalent, and combined vaccines. Immunized hen eggs were collected after the third vaccination and IgYs were purified. In total, 100 mice were orally challenged with Salmonella serotypes. After the challenge, IgYs were orally administered to mice. Mice were observed for morbidity and mortality. Fecal samples from the mice were also cultured for the reisolation of Salmonella serotypes. The antibody titers in the serum samples of vaccinated chickens were higher than those …

Estrogen Receptor Alpha (Esr1)-Dependent Regulation Of The Mouse Oviductal Transcriptome, Katheryn L. Cerny, Rosanne A. C. Ribeiro, Myoungkun Jeoung, Chemyong Ko, Phillip J. Bridges

Animal and Food Sciences Faculty Publications

Estrogen receptor-α (ESR1) is an important transcriptional regulator in the mammalian oviduct, however ESR1-dependent regulation of the transcriptome of this organ is not well defined, especially at the genomic level. The objective of this study was therefore to investigate estradiol- and ESR1-dependent regulation of the transcriptome of the oviduct using transgenic mice, both with (ESR1KO) and without (wild-type, WT) a global deletion of ESR1. Oviducts were collected from ESR1KO and WT littermates at 23 days of age, or ESR1KO and WT mice were treated with 5 IU PMSG to stimulate follicular development and the production of ovarian estradiol, and the …

Measuring The Role Of Inhibition In Auditory Discrimination In Mice, Tazima Nur

Graduate Theses and Dissertations

Understanding how inhibitory neurons affect sensory information processing in the cerebral cortex is an ongoing goal of both neuroscience and statistical physics research. In this master's thesis research project, an experimental system has been designed and built for studying how auditory dynamic range depends on inhibitory neurons, based on observations of mouse behavior. In this thesis, firstly, the topic of inhibition and information processing has been introduced. Then two papers related to inhibition and dynamic range has been reviewed in detail. One of the papers is an experimental work that analyzes the affect of inhibition on dynamic range. The other …

Prolonged Pain Research In Mice: Trends In Reference To The 3rs, Jonathan Balcombe, Hope Ferdowsian, Lauren Briese

Jonathan Balcombe, PhD

This literature review documents trends in the use of mice in prolonged pain research, defined herein as research that subjects mice to a source of pain for at least 14 days. The total amount of prolonged pain research on mice has increased dramatically in the past decade for the 3 pain categories examined: neuropathic, inflammatory, and chronic pain. There has also been a significant rise in the number of prolonged mouse pain studies as a proportion of all mouse studies and of all mouse pain studies. The use of transgenic mice has also risen significantly in prolonged pain research, though …

Limitations On Spatial Memory In Mice, Robert H.I. Dale, Martin Bedard

Robert H. I. Dale

Rats have an impressive ability to remember locations they have visited. Two experiments used an eight-arm radial maze to determine whether mice showed two important characteristics of this spatial memory: its durability, and its dependence on stimuli outside the maze (extreme stimuli). In Experiment 1, food-deprived mice were allowed to eat from four of the eight arms of the maze then, after delays of 5 sec, 1 min, or 5 min, they were permitted to choose the remaining arms. Choice accuracy declined significantly with the longer delays, but always remained above chance. In Experiment 2, the maze was rotated 180° …

Lifecycle Efficiency Of Mice Divergently Selected For Heat Loss, Adrienne Bhatnagar

Department of Animal Science: Dissertations, Theses, and Student Research

Divergent selection for heat loss as an indication of maintenance energy requirements in mice resulted in a low (ML) and high maintenance line (MH), and an intermediate control line (MC). Improved feed efficiency has been observed in ML mice, which would be economically beneficial in livestock species. However, previous work showed evidence of unfavorable correlated responses to selection in ML mice compared to MH mice. Therefore, the objective of this study was to model a lifecycle similar to a livestock production system using these lines and determine if these correlated responses diminish the benefit of improved feed efficiency by calculating …

Bioengineered Lysozyme Reduces Bacterial Burden And Inflammation In A Murine Model Of Mucoid Pseudomonas Aeruginosa Lung Infection, Charlotte C. Teneback, Thomas C. Scanlon, Matthew J. Wargo, Jenna L. Bement, Karl E. Griswold, Laurie W. Leclair

Dartmouth Scholarship

The spread of drug-resistant bacterial pathogens is a growing global concern and has prompted an effort to explore potential adjuvant and alternative therapies derived from nature's repertoire of bactericidal proteins and peptides. In humans, the airway surface liquid layer is a rich source of antibiotics, and lysozyme represents one of the most abundant and effective antimicrobial components of airway secretions. Human lysozyme is active against both Gram-positive and Gram-negative bacteria, ac

The Micronutrient Profile Of The Typical American Diet Enhances Colorectal Carcinogenesis, Stephany Del Carmen Perez Monsanto

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The typical Western dietary pattern is characterized by the consumption of energy-dense, nutrient-poor foods and has been linked to increased risk of colorectal cancer (CRC). Our research group previously developed the total Western diet (TWD) that emulates typical human dietary intakes of macro- (carbohydrates, proteins, and fats) and micronutrients (vitamins and minerals) on an energy density basis for rodents. In the present study, we sought to determine the impact of TWD on biomarkers of metabolic syndrome and obesity in comparison to a commercial 45% fat diet used for models of diet-induced obesity (DIO diet) and the standard basal AIN93G diet, …

Evaluation Of The Pathogenicity Of Candida Zeylanoides In Balb/C Mice, Alireza Khosravi, Hojjatollah Shokri, Donya Nikaein, Ahmad Erfanmanesh, Mahnaz Fatahinia, Jalal Ashrafi Hlan

Turkish Journal of Veterinary & Animal Sciences

Systemic candidiasis is an opportunistic infection caused by Candida species in animals. The purpose of this study was to evaluate the pathogenicity of different doses of Candida zeylanoides in BALB/c mice. Thirty mice were selected in this study. Different doses of the yeast were intravenously inoculated to the animals. At first, clinical signs and survival time of infected mice were recorded. Then both mycological and histological examinations were performed for detection of Candida in different tissues. The results showed that the injection of 1 × 10^8 cells of C. zeylanoides caused high mortality (group 1). The mortalities occurred within 7-12 …

Renewed Selection For Heat Loss In Mice: Direct Responses And Correlated Responses In Feed Intake, Body Weight, Litter Size, And Conception Rate, J. M. Mcdonald, Merlyn K. Nielsen

Department of Animal Science: Faculty Publications

Divergent selection in mice was renewed in 3 independent replicates for high (MH) and low (ML) heat loss. An unselected control (MC) was maintained in all replicates. Heat loss was measured for individual male mice for 15 h, overnight in direct calorimeters. After 16 initial generations of selection followed by 26 generations of relaxed selection, divergent selection resumed for 9 generations. The realized selection applied was very close to the maximum possible selection according to the criteria and protocol. Selection differentials were greater for high than for low selection due to greater variation in the MH line. When corrected for …

Measuring Ultrasonic Communication Between Mouse Pups And Adult Mother Mice, Katie Ludwig

Undergraduate Research Symposium (UGRS)

Measuring ultrasonic communication provides us with a way to study parental influence on animals. In this study I measured the ultrasonic communication between mouse pups and two maternal females, one of which who had given birth to the pups and the other had raised them. I found that there was no significant difference between the amount of noise expressed by pups in response to each the biological mother and foster mother test groups. Mouse pups call to maternal females regardless of genetic relatedness. Communication in mice may be a more complicated model because of their communal nature.

Efficacy Of Orally Administered T-705 On Lethal Avian Influenza A (H5n1) Virus Infections In Mice, R. W. Sidwell, Dale L. Barnard, C. W. Day, Donald F. Smee, K. W. Bailey, M. H. Wong, John D. Morrey, Y. Furuta

John D. Morrey

T-705 (6-fluoro-3-hydroxy-2-pyrazinecarboxamide) was inhibitory to four strains of avian H5N1 influenza virus in MDCK cells, with the 90% effective concentrations ranging from 1.3 to 7.7 µM, as determined by a virus yield reduction assay. The efficacy was less than that exerted by oseltamivir carboxylate or zanamivir but was greater than that exerted by ribavirin. Experiments with mice lethally infected with influenza A/Duck/MN/1525/81 (H5N1) virus showed that T-705 administered per os once, twice, or four times daily for 5 days beginning 1 h after virus exposure was highly inhibitory to the infection. Dosages from 30 to 300 mg/kg of body weight/day …

Comparison Of Feed Energy Costs Of Maintenance, Lean Deposition, And Fat Deposition In Three Lines Of Mice Selected For Heat Loss, D. L. Eggert, Merlyn K. Nielsen

Department of Animal Science: Faculty Publications

Three replications of mouse selection populations for high heat loss (MH), low heat loss (ML), and a nonselected control (MC) were used to estimate the feed energy costs of maintenance and gain and to test whether selection had changed these costs. At 21 and 49 d of age, mice were weighed and subjected to dual x-ray densitometry measurement for prediction of body composition. At 21 d, mice were randomly assigned to an ad libitum, an 80% of ad libitum, or a 60% of ad libitum feeding group for 28-d collection of individual feed intake. Data were analyzed using 3 approaches. …

A Porphyrin Increases Survival Time Of Mice After Intracerebral Prion Infection, D. A. Kocisko, W. S. Caughey, R. E. Race, G. Roper, B. Caughey, John D. Morrey

John D. Morrey

Prion diseases, including scrapie, are incurable neurodegenerative disorders. Some compounds can delay disease after a peripheral scrapie inoculation, but few are effective against advanced disease. Here, we tested multiple related porphyrins, but only Fe(III)meso-tetra(4-sulfonatophenyl)porphine injected into mouse brains after intracerebral scrapie inoculation substantially increased survival times.

Evaluation Of Immunomodulators, Interferons And Known In Vitro Sars-Cov Inhibitors For Inhibition Of Sars-Cov Replication In Balb/C Mice, Dale L. Barnard, C. W. Day, K. Bailey, M. Heiner, R. Montgomery, L. Lauridsen, P. K-S. Chan, R. W. Sidwell

Animal, Dairy, and Veterinary Science Faculty Publications

Compounds approved for therapeutic use and in vitro inhibitors of severe acute respiratory syndrome coronavirus (SARS-CoV) were evaluated for inhibition in the mouse SARS-CoV replication model. A hybrid interferon, interferon alpha (IFN-alpha) B/D, and a mismatched double-stranded (ds) RNA interferon (IFN) inducer, Ampligen® (poly I:poly C124), were the only compounds that potently inhibited virus titres in the lungs of infected mice as assessed by CPE titration assays. When mice were dosed intraperitoneally (i.p.) with IFN-alpha B/D once daily for 3 days beginning 4 h after virus exposure, SARS-CoV replication in the lungs of infected mice was reduced by 1 log10 …