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Full-Text Articles in Life Sciences

Expression Of Lysine-Mediated Neuropeptide Hormones Controlling Satiety And Appetite In Broiler Chickens, Collins N. Khwatenge, Boniface M. Kimathi, Thyneice Taylor-Bowden, Samuel N. Nahashon Dec 2019

Expression Of Lysine-Mediated Neuropeptide Hormones Controlling Satiety And Appetite In Broiler Chickens, Collins N. Khwatenge, Boniface M. Kimathi, Thyneice Taylor-Bowden, Samuel N. Nahashon

Agricultural and Environmental Sciences Faculty Research

Lysine is the second most limiting amino acid after methionine and is considered the most limiting amino acid for growth in poultry. Lysine requirement for broiler chickens has changed over the years. Leptin and adiponectin represent 2 adipokines that mediate metabolism by eliciting satiety effects whereas ghrelin peptide hormone influences appetite. We hypothesize that this affects growth performance of chicks. This study evaluates the effect of varying dietary lysine homeostasis on performance of broiler chickens through satiety- and appetite-mediating hormones. In 3 replications, 270 one-day-old chicks were reared for 8 wk feeding on diets comprising 0.85, 1.14, and 1.42% lysine …


The Identification Of Differentially Expressed Genes Between Extremes Of Placental Efficiency In Maternal Line Gilts On Day 95 Of Gestation, Shanice K. Krombeen, Vijay Shankar, Rooksana E. Noorai, Christopher A. Saski, Julia L. Sharp, Matthew E. Wilson, Tiffany A. Wilmoth Jan 2019

The Identification Of Differentially Expressed Genes Between Extremes Of Placental Efficiency In Maternal Line Gilts On Day 95 Of Gestation, Shanice K. Krombeen, Vijay Shankar, Rooksana E. Noorai, Christopher A. Saski, Julia L. Sharp, Matthew E. Wilson, Tiffany A. Wilmoth

Faculty & Staff Scholarship

Background: Placental efficiency (PE) describes the relationship between placental and fetal weights (fetal wt/placental wt). Within litters, PE can vary drastically, resulting in similarly sized pigs associated with differently sized placentas, up to a 25% weight difference. However, the mechanisms enabling the smaller placenta to grow a comparable littermate are unknown. To elucidate potential mechanisms, morphological measurements and gene expression profiles in placental and associated endometrial tissues of high PE and low PE feto-placental units were compared. Tissue samples were obtained from eight maternal line gilts during gestational day 95 ovario-hysterectomies. RNA was extracted from tissues of feto-placental units with …


Endotoxin Increases Oxidative Stress And Oxygen Tension While Reducing Milk Protein Gene Expression In The Mammary Gland, Alexander Jonathan Spitzer Jan 2019

Endotoxin Increases Oxidative Stress And Oxygen Tension While Reducing Milk Protein Gene Expression In The Mammary Gland, Alexander Jonathan Spitzer

Graduate College Dissertations and Theses

Mastitis, the inflammation of the mammary gland by bacterial infection, is one of the costliest diseases to the dairy industry primarily due to a loss in milk production. The aim of this study was to investigate the mechanisms underlying reduced milk production during mastitis. We hypothesized that bacterial endotoxin induces cell apoptosis, oxidative stress and increases hypoxia while inhibiting milk gene expression in the mammary gland. To test this hypothesis, mice were bred to pregnancy, and 3 days post-partum the left and right sides of the 4th pair of mammary glands were alternately injected with either the endotoxin liposaccharide (LPS, …


Acute And Chronic Effects Of Thifluzamide On Daphnia Magna, Feyza İçoğlu Aksakal Jan 2019

Acute And Chronic Effects Of Thifluzamide On Daphnia Magna, Feyza İçoğlu Aksakal

Turkish Journal of Zoology

Thifluzamide is a thiazole carboxanilide fungicide used to control a wide range of basidiomycete diseases on maize, potatoes, rice, and other crops. Although this fungicide is found in aquatic environments, there is no information about its acute and chronic toxicity on aquatic invertebrates. In the present study, to investigate acute and chronic toxicity of thifluzamide, daphnids were exposed to 1.25, 1.5, 2, and 2.5 mg/L thifluzamide for 48 h and 21 days, respectively. The expression of four oxidative stress-related genes (CAT, GCLC, GST, and GPX) and life-history parameters such as days to the first brood, days to the first egg …


Novel Approaches To Evaluate And Enhance Neonatal Calf Gastrointestinal Health And Development, Fernanda Trindade Da Rosa Jan 2019

Novel Approaches To Evaluate And Enhance Neonatal Calf Gastrointestinal Health And Development, Fernanda Trindade Da Rosa

Electronic Theses and Dissertations

Physiological adaptations of the gastrointestinal tract (GIT) epithelial cells as well as methods to manipulate early life GIT microbiota colonization during the neonatal stage, is of great importance to the dairy industry. The first objective of this research was to optimize a method based on evaluation of bovine transcripts in fecal RNA via RTqPCR using L-selectin (SELL) as a marker for polymorphonuclear leukocytes (PMNL), keratin 8 (KRT8) and fatty acid binding protein 2 (FABP2) for GIT enterocytes, and tight junctions in fecal RNA and GIT sections in dairy calves. To test the reliability of the fecal RNA method, fecal and …