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Full-Text Articles in Life Sciences

A Road Map For Place Based Collaboration For Conflict Reduction, Joseph L. Zecher Jan 2024

A Road Map For Place Based Collaboration For Conflict Reduction, Joseph L. Zecher

Undergraduate Theses, Professional Papers, and Capstone Artifacts

No abstract provided.


Effects Of Supplemental Trace Minerals As Amino Acid Complexed Or Inorganic Sources For Beef Cattle From Receiving Through Finishing, Robin Ashley Cheek Aug 2023

Effects Of Supplemental Trace Minerals As Amino Acid Complexed Or Inorganic Sources For Beef Cattle From Receiving Through Finishing, Robin Ashley Cheek

Graduate Theses and Dissertations

The objective of experiments was to investigate the effects of inorganic or amino acid-complexed sources of trace minerals on health, gain performance, liver mineral concentrations, and carcass characteristics of beef heifers from receiving through finishing. To investigate the effects of inorganic or amino acid-complexed sources of trace minerals (zinc, copper, manganese, and cobalt) on performance and morbidity of beef heifers during the receiving period, crossbred beef heifer calves arriving on 3 delivery dates were used in a 42-day receiving trial. Treatments consisted of supplemental zinc (360 mg/d), copper (125 mg/d), manganese (200 mg/d), and cobalt (12 mg/d) from complexed (Availa-4, …


A Mechanistic Examination Of Interspecific Competition Between Wild And Domestic Herbivores, Courtney Check Aug 2023

A Mechanistic Examination Of Interspecific Competition Between Wild And Domestic Herbivores, Courtney Check

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Large herbivores, such as mule deer and cattle have similar life histories and likely compete for resources. However, quantifying the extent to which these species compete and the specific resources they compete for has proved challenging. My research examines if cattle influence deer abundance and behavior due to competition for forage, competition for shade, and/or by affecting the predation risk of deer. Using a grid of autonomous trail cameras, I was able to determine if cattle abundance influences local deer abundance in relation to specific resources and habitat features. Using GPS data from collared deer, I was also able to …


Identifying Barriers To Data Use On U.S. Beef Cow-Calf Operations And Developing Solutions To Improve Cow-Calf Record-Keeping, William Isaac Jumper May 2023

Identifying Barriers To Data Use On U.S. Beef Cow-Calf Operations And Developing Solutions To Improve Cow-Calf Record-Keeping, William Isaac Jumper

Theses and Dissertations

Cattle health and production records (CHPR) are data collected by cattle producers and veterinarians in the form of measurements, observations, counts of events over time, and physiologic attributes that describe individual and group-level health and production. These data are useful to both veterinarians and cattle producers for making evidence-based decisions on cow-calf operations. Currently, there are no uniform, industry-wide methods of capturing and recording CHPR in the U.S. cow-calf industry. Although many cow-calf producers in the U.S. are thought to collect some form of CHPR, it is believed that relatively few are doing so in an electronic manner that facilitates …


Pharmacokinetics Of Single Feeding Of Cannabidiol In Cattle: A Pilot Study, Haley Cornette Apr 2023

Pharmacokinetics Of Single Feeding Of Cannabidiol In Cattle: A Pilot Study, Haley Cornette

Honors College Theses

Cannabidiol (CBD) is a substance that has been used in complementary medicine for many years. However, modern medicine has little knowledge of how this substance is utilized and metabolized in ruminant animals. Regulations on quality assurance and use in animals are lacking, and CBD supplementation in livestock is not approved. If CBD supplements can be shown to be safe and effective, detection in the animal will be important for determining regulation of use. A withdrawal period can then be established to allow time for deterioration of product to safe levels before livestock products enter human markets. This study sought to …


Effects Of Biosynthesized Bromoform On Enteric Methane Production, Animal Performance And Tissue Residues In Cattle, Sydney Davis Jan 2023

Effects Of Biosynthesized Bromoform On Enteric Methane Production, Animal Performance And Tissue Residues In Cattle, Sydney Davis

Theses and Dissertations--Animal and Food Sciences

Greenhouse gas (GHG) emissions have been implicated in global warming and extreme climate change conditions. The Environmental Protection Agency (EPA) has identified agriculture, more specifically cattle production, as a major contributor to global GHG production. When expressed as liters of gas, CO2 is considered the primary GHG produced, however, in terms of global warming potential (GWP), CH4 has 25-times greater potential than that of CO2. Due to the elevated GWP of CH4, we have constructed a series of experiments to investigate the use of a halogenated CH4 analogue derived from kelp (i.e., bromoform) as an inhibitor of enteric CH4 production …


Potentials Of Pleurotus: Reimagining The Relationship Between Cattle And Brewer's Spent Grain, Zoe Stojkovic Jan 2023

Potentials Of Pleurotus: Reimagining The Relationship Between Cattle And Brewer's Spent Grain, Zoe Stojkovic

Senior Projects Spring 2023

Brewer’s spent grains (BSGs), a byproduct from beer production, are generated in excess globally. Most often, they are sold or given to proximate cattle farmers for use as feed. However, spent grain can also be used as a medium for fungal cultivation. Given that certain fungal species have the capability to degrade lignin and produce protein, the cultivation of fungi on spent grains may serve to enhance the nutritional profile of the grains for their use as cattle feed. This project is an effort to determine the compatibility of fungal cultivation with BSGs in order to both improve upon cattle …


Incorporating Follicle Stimulating Hormone To Stimulate Multiple Corpora Lutea For Embryo Transfer In Beef Cattle, Bryan Carter Aug 2022

Incorporating Follicle Stimulating Hormone To Stimulate Multiple Corpora Lutea For Embryo Transfer In Beef Cattle, Bryan Carter

Theses and Dissertations

Assisted reproductive technologies, such as estrus synchronization and embryo transfer, can aid producers in improving genetics by increasing the number of progeny produced from elite females. The success of embryo transfer is dependent on a viable, competent embryo and a recipient with a receptive uterine environment. Follicular development and luteinization are pertinent for the recipient to establish a functional corpus luteum (CL) that can produce adequate concentrations of progesterone (P4) and provide a uterine environment conducive for the establishment of pregnancy. The objective of this study was to determine if exogenous follicle stimulating hormone (FSH), would increase the number of …


Optimal Stocker Production Strategies For Spring And Fall Calving Cow Herds, Cora Beth Key Aug 2022

Optimal Stocker Production Strategies For Spring And Fall Calving Cow Herds, Cora Beth Key

Masters Theses

This thesis consists of two chapters discussing the economics of raising stocker beef cattle in the southeast United States. The objective of the first chapter is to estimate supplemental feed cost for spring-calving cows, spring-born stockers, cows calving in the fall, and fall-born stockers, while considering the seasonality of forage production and nutritional needs. The chapter explores ways producers can lower their feed costs and demonstrates the importance of managing hay expenses for cattle producers to control cost. The objective of chapter two is to determine the profit-maximizing stocker period in an integrated cow-calf and stocker operation with both fall- …


Effects Of Providing Novel Feedstuffs To Livestock On Production And Skeletal Muscle Growth, Laura A. Motsinger May 2022

Effects Of Providing Novel Feedstuffs To Livestock On Production And Skeletal Muscle Growth, Laura A. Motsinger

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

As the population increases and available land for food production decreases, it is necessary for livestock producers to continually work towards increasing livestock production efficiency. In livestock operations, feed accounts for the majority of input costs associated with raising livestock. As such, it is necessary to improve growth and production of livestock animals, while also optimizing feed utilization. Different feedstuffs can be included in the diet of livestock animals to maximize growth and production. However, the effects of some of these novel feedstuffs on growth and production of livestock animals has not been elucidated. As such, we investigated the effects …


Effects Of Cannabinoid Supplementation On Weaning And Transportation Stress In Beef Calves, Savannah Kaitlyn Austin Jan 2022

Effects Of Cannabinoid Supplementation On Weaning And Transportation Stress In Beef Calves, Savannah Kaitlyn Austin

Murray State Theses and Dissertations

Transportation stress is a major factor increasing the risk of bovine respiratory disease (BRD) in young calves. Bovine respiratory disease is currently the most economically important disease affecting the cattle industry, costing producers millions of dollars annually. Cannabidiol (CBD) has shown therapeutic benefits in other species, making it a potential tool to use as a supplement to reduce transportation and weaning stress in calves. However, little information is available on cannabinoid bioavailability and disposition of bioactive residue in livestock tissues. The objectives of these studies were to evaluate effective dosage rates of a cannabinoid gel on beef cattle and to …


Optimising The Carcass Merit Of Irish Beef Cattle Using Genetic And Non-Genetic Information At The Animal And Herd Level, David Kenny Jan 2022

Optimising The Carcass Merit Of Irish Beef Cattle Using Genetic And Non-Genetic Information At The Animal And Herd Level, David Kenny

Theses

Failure of beef carcasses to achieve desirable carcass specifications represent inefficiencies within the supply chain, namely greater carcass processing costs and the inability of the resulting primal cuts to conform to high-value market specifications. Analysis of a representative sample of prime Irish beef cattle conducted in this thesis determined that 59% of cattle fail to achieve the desired carcass specifications of the supply chain at slaughter. The objective of this thesis was to use readily available information to define strategies that could help to reduce this statistic. Firstly, the likelihood of Irish beef carcasses achieving the desired carcass specifications was …


Escherichia Coli And Salmonella Sp. In Domestic Cattle And Wild Roosevelt Elk: Fecal Pathogens At The Wildlife-Domestic Interface, Emily A. Buck Jan 2022

Escherichia Coli And Salmonella Sp. In Domestic Cattle And Wild Roosevelt Elk: Fecal Pathogens At The Wildlife-Domestic Interface, Emily A. Buck

Cal Poly Humboldt theses and projects

Direct or indirect contact between domestic populations of animals and wildlife carries an inherent risk for transmission of pathogens that cause infectious disease. In Humboldt and Del Norte counties of northern California, ongoing conflict between ranchers and Roosevelt elk groups results from elk use of ranching pastures and pastures on private land. Fecal samples from elk in association with cattle, cattle, and from elk not in known association with cattle were assessed for the presence of bacteria Salmonella enterica and pathogenic Escherichia coli to assess whether association with cattle increases risk of infection for elk. Group identity (one of the …


Cattle Producer Motivations And Willingness To Participate In Industry Animal Welfare Program Verification, Chase Decoite Jun 2021

Cattle Producer Motivations And Willingness To Participate In Industry Animal Welfare Program Verification, Chase Decoite

Master's Theses

As consumers have become increasingly interested in where their food comes from and how it is grown, the cattle and beef industry is without exception. Many consumers are beginning to question traditional animal husbandry practices and on-farm animal welfare protocol. It is essential that the food system meets new demands and understands consumer views. In order to address these concerns, the industry has rallied around the Beef Quality Assurance (BQA) program to set the standard for cattle welfare and beef quality, with the explicit goal of improving consumer confidence. The goal of this research is to determine if cattle producers …


Exploring How Maternal Phosphorus Status Affects Calf Growth And Performance, Elizabeth Lafferty May 2021

Exploring How Maternal Phosphorus Status Affects Calf Growth And Performance, Elizabeth Lafferty

Animal Science Undergraduate Honors Theses

This study will focus on how maternal phosphorus status of beef heifers affects the growth and performance of their calves. Heifers have been offered free-choice mineral with either 0 or 4% supplemental phosphorus from 30 days after weaning until calving. A study by H. Hilfiker, a University of Arkansas honors student, investigated the effects of these treatments from 30 days after weaning until 60 days after the breeding season when heifers were confirmed to be bred or open. For this developing heifer project 64 crossbred Angus heifers were assigned randomly into 8 groups (8 heifers/ group) before assigning each group …


The Effect Of Endophyte-Infected Tall Fescue Seed Consumption On Gut And Satiety Hormones Related To Intake Regulation In Holstein Steers, Mindy Elizabeth King Jan 2021

The Effect Of Endophyte-Infected Tall Fescue Seed Consumption On Gut And Satiety Hormones Related To Intake Regulation In Holstein Steers, Mindy Elizabeth King

Theses and Dissertations--Animal and Food Sciences

Cattle consuming endophyte-infected tall fescue (E+) typically experience a syndrome termed fescue toxicosis which is thought to be caused by ergot alkaloids produced by the endophyte. The most abundant alkaloid, and considered the most likely cause of the syndrome, is ergovaline (ERV). During fescue toxicosis, a decrease in ADG is observed which is likely due to the decrease in DMI commonly observed in animals consuming E+ compared to animals consuming non-endophyte-infected tall fescue (E-). However, the cause of the decrease in intake is not well elucidated. Many physiological responses control feed intake including, but not limited to, physical, neural, metabolic, …


Impact Of Mineral Supplementation On Intake Behavior And Blood Flow In Growing Beef Cattle Exposed To Fescue-Derived Alkaloids, Hannah Marie Herzing Jan 2021

Impact Of Mineral Supplementation On Intake Behavior And Blood Flow In Growing Beef Cattle Exposed To Fescue-Derived Alkaloids, Hannah Marie Herzing

Theses and Dissertations--Animal and Food Sciences

Consumption of endophyte-infected tall fescue by cattle has repeatedly been shown to negatively impact dry matter intake (DMI), growth rate, and circulating prolactin concentrations. The objective of the current study was to determine if a mineral supplementation would mitigate the negative effects of fescue-derived alkaloid consumption on feed intake, circulating prolactin, and the vascular system. Twelve Angus crossbred steers were used in a triplicated Latin square design consisting of three 28 d experimental periods. Treatments consisted of three top-dressed mineral supplements (142 g/head/d): a non-medicated control (CON), commercially available Fescue EMT® Mineral Defense (EMT), and a test prototype (EMT 2). …


A Survey Of Anthelmintic Resistance In Angus Bulls Participating In Centralized Performance Tests In Appalachia, Roger Tyler Rohrbaugh Jan 2021

A Survey Of Anthelmintic Resistance In Angus Bulls Participating In Centralized Performance Tests In Appalachia, Roger Tyler Rohrbaugh

Graduate Theses, Dissertations, and Problem Reports

Resistance to anthelmintics, more commonly referred to as “de-wormers,” has been on the rise in parasites that affect cattle. Parasite infection can have severe impacts on calf productivity resulting in reduced profitability. Therefore, we sought to better understand anthelmintic resistance in Appalachia by surveying Angus bulls entering performance tests in VA and WV. During a four-year period, over 1050 Angus bulls were tested at bull development facilities in Wardensville, WV, Culpeper, VA and Wytheville, VA. Fecal samples were collected at arrival and 14-21 days later for determination of fecal egg count (FEC) reduction. Upon arrival, all bulls received either fenbendazole …


The Surveillance Of Gastrointestinal Parasitic Nematodes Of Northwest Arkansas Dairy Cattle Using Traditional And Genetic Parasitological Identification Procedures, Eva M. Wray Dec 2020

The Surveillance Of Gastrointestinal Parasitic Nematodes Of Northwest Arkansas Dairy Cattle Using Traditional And Genetic Parasitological Identification Procedures, Eva M. Wray

Graduate Theses and Dissertations

Traditional and genetic parasitological identification procedures were compared using naturaland artificial nematode parasite infections in Holstein steer calves. The traditional parasitological procedures measured fecal egg counts, coprocultures with subsequent larval collection and adult nematodes collected at necropsy. The genetic identification procedures measured ITS-2 sequences extracted from different stages of nematode development: raw feces, concentrated nematode eggs, third stage larvae and adults. The primary nematodes observed were Cooperia oncophora, Cooperia punctata and Ostertagia ostertagi. The traditional techniques were not significantly different from one another, while the genetic sequencing showed variation amongst the different procedures. The raw feces sequences showed the most …


Demographics, Accuracy, And Impact Of Feed Laboratories In The United States, Jerald H. Severe Dec 2020

Demographics, Accuracy, And Impact Of Feed Laboratories In The United States, Jerald H. Severe

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Feed analysis is very important to modern society. In the United States feed analysis is used to optimize production of food animals. Feed analysis is also used as a tool to place value on crops. As important as feed analysis is to society, little research has been done that describes which feed laboratories are the most popular and why people use them. It has been thought by some patrons that different results from the same feed sample are obtained by different laboratories. Is this true? If so, what is the effect on those that use feed laboratories to produce animals, …


Biogeography Of The Bovine Respiratory Microbiome And Its Relationship With Bovine Respiratory Disease, Jianmin Chai Dec 2020

Biogeography Of The Bovine Respiratory Microbiome And Its Relationship With Bovine Respiratory Disease, Jianmin Chai

Graduate Theses and Dissertations

Bovine respiratory disease (BRD) is the most common and costly disease in the beef cattle industry, leading to high morbidity, mortality and huge economic loss. Despite the recent advances in vaccination and antimicrobial techniques, no significant health-improved outcomes have developed. Due to a deep investigation of the microbiome, respiratory microbiotas are known to have important roles for host health and disease. However, BRD specific pathogens have not yet been identified since they are found in both healthy and diseased animals. A systemic and comprehensive study of the biogeography of the bovine respiratory microbiome and its relationship with BRD is lacking …


Predicting Voluntary Forage Intake Of Supplemented Beef Cattle, Connor Biehler Dec 2020

Predicting Voluntary Forage Intake Of Supplemented Beef Cattle, Connor Biehler

Masters Theses

A major priority of beef cattle production is to meet animal nutrient requirements in order to achieve a desired level of productivity. Accurately predicting voluntary forage intake (VFI) is necessary to accurately predict the total nutrient intake of grazing or forage-fed beef cattle that are also supplemented with other sources of nutrients. Therefore, the objectives of this experiment were to utilize data from published literature to 1) identify factors that explain variation in VFI, and 2) develop and validate one or more mathematical models that predict VFI or total nutrient intake of grazing or forage-fed and supplemented beef cattle. A …


From Fields To Factories: The Industrialization Of The United States’ Cattle Industry, Joseph Petersen May 2020

From Fields To Factories: The Industrialization Of The United States’ Cattle Industry, Joseph Petersen

History | Senior Theses

This paper will look at the changes of the United States of America's cattle and beef industry from the 19th into the 21st century. It will also show how the industry has evolved into its current state and predict the changes to come. This paper will be evaluating how technology and equipment have changed the traditional farming and ranch lifestyles. While also breaking down the economies from pre-industrial times into modern day. This paper will also explore the effect that technology, equipment, ranching styles, labor and financial changes had on the cattle and beef industry. Finally, this paper will prove …


Effects Of Lasalocid And Energy Supplementation On Forage Intake, Energy Metabolism, And Performance Of Cattle Grazing Wheat Pasture, Autumn Tayler Pickett May 2020

Effects Of Lasalocid And Energy Supplementation On Forage Intake, Energy Metabolism, And Performance Of Cattle Grazing Wheat Pasture, Autumn Tayler Pickett

Graduate Theses and Dissertations

Cattle grazing wheat pasture have the potential to gain BW exceptionally well, but excessive nitrogen intake results in increased excretion and increased greenhouse gas (GHG) emissions. Supplemental concentrates with the addition of an ionophore given to ruminants grazing wheat is a potential practice for producers to increase nitrogen efficiency while decreasing GHG emissions. Therefore, the objective of this experiment was to quantify the effects of energy (2.95 kg/d) and lasalocid (200 mg/hd/d) supplementation on nutrient intake, energy metabolism, respiratory gas fluxes, and performance of grazing cattle. Methane emissions were not affected (P = 0.58) by treatment, hence methane intensity (P …


Associations Among Beef Cattle Genotypes, Neospora Caninum Infection, And Reproductive Performance, Ryan James Page May 2020

Associations Among Beef Cattle Genotypes, Neospora Caninum Infection, And Reproductive Performance, Ryan James Page

Graduate Theses and Dissertations

Reproductive performance is crucial for sustained financial success in the beef cattle industry. This dissertation includes a population study that quantified the incidence of Neospora caninum infections in the central region of the United States and tested its relationship with reproductive performance in beef cattle. Trial one of that study concluded that 6.9% of open, replacement heifers (n = 1306) tested seropositive. The second trial in that project found that 9.6% of the breeding age females (n = 500) tested were seropositive for Neospora caninum; and that state in which the cattle lived and age impacted (P < 0.05) infection rate. Breed composition, number of farm dogs on the ranch, and use of total mixed rations were not associated (P > 0.1) with seropositive …


Technologies To Increase Animal Performance In Beef Production Systems, Caleb P. Weiss Aug 2019

Technologies To Increase Animal Performance In Beef Production Systems, Caleb P. Weiss

Graduate Theses and Dissertations

Three monensin levels during a stocker phase (0, 800, 1600 g/ton in a free choice mineral) and two levels during finishing (0 [U] or 37.5 mg/kg diet DM [M]) were used to determine the effects of monensin supplementation during a stocker and subsequent finishing phase on beef calves. Steers (n = 605, BW = 278 ± 27 kg) were fed pearl millet hay with soybean hull and corn gluten feed supplement (0.5% BW daily [AF basis] Block 1) or grazed fall wheat (Block 2), spring wheat (Block 3), bermudagrass (Blocks 4 & 5) or wheat with mixed-grass baleage (Block 6). …


Determining The Relationship Among Cattle Genotype, Hair Coat Score, And Productivity Through The Investigation Of Single Nucleotide Polymorphisms Within Prolactin, Dopamine Receptor D2, And Melatonin Receptor 1a, Erin Davis May 2019

Determining The Relationship Among Cattle Genotype, Hair Coat Score, And Productivity Through The Investigation Of Single Nucleotide Polymorphisms Within Prolactin, Dopamine Receptor D2, And Melatonin Receptor 1a, Erin Davis

Animal Science Undergraduate Honors Theses

Prolactin (PRL), melatonin (MTN), and dopamine (DA) are all hormones that are believed to play a role in the regulation and growth of hair in beef cattle. There are also single nucleotide polymorphisms associated with each of these hormones or their receptors, indicating that the investigation of these polymorphisms could allow them to serve as genetic markers for the future productivity of an animal. The objective of this study was to determine the relationships among cattle genotype, hair coat score, and productivity through the investigation of single nucleotide polymorphisms within prolactin, dopamine receptor D2, and melatonin receptor 1A. Body weights, …


Relationships Between Animal Temperament And Systemic Immune Responses In Beef Cattle Exposed To Conditions Associated With Conventional Management, Alexander W. Altman Jan 2019

Relationships Between Animal Temperament And Systemic Immune Responses In Beef Cattle Exposed To Conditions Associated With Conventional Management, Alexander W. Altman

Theses and Dissertations--Animal and Food Sciences

Measures of temperament have been shown to influence physiological responses. Exit velocity (EV) has been identified as an objective, robust measure of temperament that can be used to predict subsequent performance of cattle. Additionally, previous studies from our lab indicate this measure of temperament may be related to production of interferon-γ (IFN-γ), a cytokine associated with cell-mediated immunity (CMI). Whereas research has investigated effects of EV upon immune responses, the overall goal of these studies was to examine this relationship under a variety of scenarios including human handling, transportation, and exposure to endophyte-infected tall fescue (E+) for determination of its …


Optimization Of Reproductive Success In Beef Cattle By Implementation Of Estrus Resynchronization, Nutritional Management, And Evaluation Of Disease Prevalance, Kaitlin M. Epperson Jan 2019

Optimization Of Reproductive Success In Beef Cattle By Implementation Of Estrus Resynchronization, Nutritional Management, And Evaluation Of Disease Prevalance, Kaitlin M. Epperson

Electronic Theses and Dissertations

The beef industry is a unique community of operations that employ different management techniques to accomplish a similar goal. Like other facets of animal agriculture, profitability of the beef industry is heavily reliant upon the implemented management practices. Reproductive efficiency is an area of management that has great potential to positively influence the success of beef production. Through the use of reproductive technologies such as estrus synchronization and AI, a greater percentage of females can become bred to superior sires, breeding and calving seasons can be shortened, calf uniformity increased, and economic return maximized (Odde, 1990; Rodgers et al., 2012; …


Impacts Of Small Intestinal Enzyme Activity And Varying Corn Grain Processing Types On Feeding Behavior, And Growth Performance Of Finishing Beef Steers, Wyatt Smith Jan 2019

Impacts Of Small Intestinal Enzyme Activity And Varying Corn Grain Processing Types On Feeding Behavior, And Growth Performance Of Finishing Beef Steers, Wyatt Smith

Electronic Theses and Dissertations

The objective of this study was to quantify the differences in activity of jejunal maltase and isomaltase between 2 groups of steers with average dry matter intake (DMI) and differing average daily gain (ADG). Dry matter intake and ADG were measured in crossbred steers (n = 69). Jejunal mucosal samples were collected from 8 steers with the greatest (high) or least (low) ADG and average DMI (± 0.55 standard deviation). Homogenates of jejunal mucosa were incubated with increasing amounts of maltose and isomaltose to determine the disaccharidase kinetics. Neither the Km of isomaltase (P = 0.15) or maltase (P = …