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Full-Text Articles in Life Sciences
Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Cw Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Et Al
Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Cw Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Et Al
VIMS Articles
Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and …
Liver Carcinogenesis In A Non-Migratory Fish: The Association With Polycyclic Aromatic Hydrocarbon Exposure, Wolfgang K. Vogelbein, M. A. Unger
Liver Carcinogenesis In A Non-Migratory Fish: The Association With Polycyclic Aromatic Hydrocarbon Exposure, Wolfgang K. Vogelbein, M. A. Unger
VIMS Articles
Field and laboratory studies indicate a strong positive association between exposure to chemical pollutants in aquatic environments and development of neoplasia in fishes. This brief communication reviews some of the more important North American and European studies that have been conducted on this relationship. We then review work conducted on a small nonmigratory estuarine cyprinodontid teleost fish, the mummichog (Fundulus heteroclitus) in the industrialized Elizabeth River, Virginia USA. Histopathological surveys of mummichogs from variously degraded habitats indicate an association between PAH exposure and development of neoplasia. We have observed non-neoplastic lesions, preneoplasms and hepatic, biliary, exocrine pancreatic and vascular neoplasms …
Molecular Detection Of Hematodinium Spp. In Norway Lobster Nephrops Norvegicus And Other Crustaceans, Hamish J. Small, Dm Neil, Ac Taylor, Et Al
Molecular Detection Of Hematodinium Spp. In Norway Lobster Nephrops Norvegicus And Other Crustaceans, Hamish J. Small, Dm Neil, Ac Taylor, Et Al
VIMS Articles
The Norway lobster Nephrops norvegicus (L.) from the coastal waters of Scotland is seasonally infected by a parasitic dinoflagellate of the genus Hematodinium. Methods used to detect infection include a morphological index (pleopod diagnosis) and several immunoassays. The present study describes the development and application of a set of Hematodinium-specific polymerase chain reaction (PCR) primers and DNA probes based on Hematodinium ribosomal DNA (rDNA). In the PCR assay, a diagnostic band of 380 bp was consistently amplified from total genomic DNA isolated from Hematodinium-infected N. norvegicus. The sensitivity of the assay was 1 ng DNA, which is equivalent to 0.6 …