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Studies On Transformation Of Tomato(Solanum Lycopersicum L.) And Arabidopsis Thaliana Using Chimerical Constructs Of Varying Tospoviral Origin, Joshua Nathaniel Cobb
Studies On Transformation Of Tomato(Solanum Lycopersicum L.) And Arabidopsis Thaliana Using Chimerical Constructs Of Varying Tospoviral Origin, Joshua Nathaniel Cobb
Theses and Dissertations
Pathogen derived resistance (PDR) is a recent breakthrough where plant hosts can be made to be resistant to viral infections through transformation with conserved viral genes. Given the severity of Tospovirus diseases worldwide (particularly in tomato), PDR has the potential to garner large yield returns where pathogen populations have overcome the established resistance. Tomato breeding lines FLA7804, FLA8044, and the research line MP1 were used in transformation experiments with potions of the Tomato spotted wilt virus (TSWV) N-gene, and two other chimerical viral nucleocapsid gene constructs from, Impatiens necrotic spot virus (INSV), and Groundnut ringspot virus (GRSV). We conducted 19 …