Life Sciences Commons^™

Cerulean Warbler (Setophaga Cerulea) And Associated Species Response To Operational Silviculture In The Central Appalachian Region, Gretchen Emily Nareff

Graduate Theses, Dissertations, and Problem Reports

In this study, I assessed the response of Cerulean Warblers (Setophaga cerulea) and 5 additional songbird species to timber harvests prescribed through operational silviculture. The research took place in relatively contiguous mature deciduous forests in 4 states in the central Appalachian region—Kentucky, Pennsylvania, Virginia, and West Virginia, USA.

For the first part of the study, I collected Cerulean Warbler abundance and territory data through point counts and territory mapping, respectively. I used the point count data to model Cerulean Warbler abundance pre- and post-harvest at 5 study areas (Kentucky [n=1], Virginia [n=2], West Virginia [n=2]) and post-harvest at …

Avian Occupancy Dynamics And Canada Warbler (Cardellina Canadensis) Environmental Correlates On A Central Appalachian Elevation Gradient In West Virginia, Gordon W. Dimmig

Graduate Theses, Dissertations, and Problem Reports

Mountains are important areas for avian diversity and conservation since they have steep elevational and environmental gradients. In the central Appalachian Mountains, several songbird species have trailing edge populations that are restricted to high elevations. Climate change and other factors can cause species distributions to change, which is of particular concern for birds in restricted trailing edge populations. Additionally, the Canada Warbler (Cardellina canadensis) breeds throughout the Appalachian Mountains and is species of conservation concern due to range-wide population declines. For this project, I used avian point count data from the central Appalachian Mountains to: (1) evaluate the …

Evaluating The Effects Of Exogenous Enzyme Supplementation On Broiler Growth, Victoria E. Ayres

Graduate Theses, Dissertations, and Problem Reports

Experiments were conducted to investigate the effects of exogenous enzymes in poultry diets. In Chapter 4, a study was conducted to determine the effects of a corn-expressed recombinant carbohydrase (AC1) on broiler performance and digesta viscosity in high non-starch polysaccharide (NSP) diets through 21 days of age. One day-old Hubbard × Cobb 500 chicks were assigned to 6 dietary treatments. Each treatment consisted of 12 replicate pens of 10 birds. The positive control diet (PC) was a corn and soybean meal formulation. The negative control diet (NC) included 10% wheat and 10% corn distiller’s dried grains with solubles (DDGS). The …

Population Health Of Spotted Salamanders (Ambystoma Maculatum) In Created Vernal Pools: An Integrative Approach, Alice R. Millikin

Graduate Theses, Dissertations, and Problem Reports

Habitat creation is an important tool for conservation to counteract habitat loss and degradation. Vernal pools are susceptible to destruction due to limited detection, protection, and regulation. These wetlands provide fishless breeding habitat for many amphibian species including spotted salamanders (Ambystoma maculatum) in eastern North America. Determining whether created vernal pool habitat is successful is often determined by demographic data of colonizing populations. I suggest that hormone levels, population genetics, and disease prevalence can improve our understanding of population health in created habitat. The goal of this dissertation was to assess the health of spotted salamander larvae in …

Demographic, Spatial, And Epigenetic Response Of The Louisiana Waterthrush (Parkesia Motacilla) To Shale Gas Development, Mack W. Frantz

Graduate Theses, Dissertations, and Problem Reports

My study centered on a bioindicator songbird, the Louisiana Waterthrush (Parkesia motacilla), hereafter waterthrush, an organism that co-occurs in both forested and aquatic habitat across the aquatic-terrestrial interface. This enabled the opportunity to quantify demographic, spatial, and epigenetic (i.e., DNA methylation) responses in a highly forested watershed of the Central Appalachians, the areas that have undergone the most rapid transformations over the last decade from unconventional shale gas development and activities. I organized my dissertation into 4 parts (Part 1: Introduction, Part 2: Louisiana Waterthrush Demography, Part 3: Spatial Assessment of Louisiana Waterthrush Foraging, Part 4: Louisiana Waterthrush …

Investigation Of Bovine Conceptus Galectins And The Effect Of Bovine Conceptus Secretory Proteins On The Endometrial Epithelial Transcriptome, Heather Lynn Baldwin

Graduate Theses, Dissertations, and Problem Reports

Pregnancy loss in cattle is greatest in the first 30 days of gestation. Near d15 of gestation, bovine conceptuses elongate and express LGALS1, an immunosuppressive glycan-binding protein believed to be essential for implantation. However, other members of the bovine galectin family, including LGALS3, LGALS4, LGALS7, LGALS8, LGALS9 and a galectin-13-like gene (LGALS13L), also may be expressed by the elongating conceptus. Therefore, galectin expression in elongating, d16 bovine conceptuses and intercaruncular endometrium as well as elongating, d14 ovine conceptuses and intercaruncular endometrium was assessed by RT-qPCR. Angus heifer and Texel ewes underwent estrous cycle synchronization and either inseminated or not bred. …

The Effect Of Bovine Galectin-1, A Conceptus Secretory Protein, On The Endometrial Transcriptome, Lindsay Faye Grose

Graduate Theses, Dissertations, and Problem Reports

Early embryonic loss in cattle is detrimental to reproduction thus, the profitability of both the beef and dairy industries. Therefore, an important aspect of study is to find techniques that beef or dairy producers could use to decrease early embryonic loss Galectin-1, the protein that was investigated in this research is important in modulating the maternal immune system in rodent and human early pregnancies by promoting fetomaternal immune tolerance. The role of galectin-1 within the reproductive tissues has not been thoroughly investigated in cattle. Galectin-1 is located in the caruncular and intercaruncular regions of the bovine endometrium, specifically the maternal …

Characterization Of Ovine Monocytes In Response To Haemonchus Contortus Larvae In Vitro And A Novel Role Of Interleukin-13 Inducing Larval Paralysis, Elizabeth Shepherd

Graduate Theses, Dissertations, and Problem Reports

Haemonchus contortus is a hematophagous, gastrointestinal parasite affecting small ruminants. Parasitism by H. contortus is the greatest concern of sheep producers in the United States and accounts for billions of dollars in economic loss worldwide. However, different breeds of sheep display differences n natural resistance to H. contortus. St. Croix hair sheep have a remarkable ability to clear H. contortus without the aid of anthelmintics and develop a robust T helper type 2 (Th2) immune response, preventing establishment of infective stage 3 larvae (L3), when compared to susceptible Suffolk sheep. However, the mechanism of development of immune response and …

Analysis Of Unconventional Oil And Gas Impacts On Downstream Fish Assemblages And Physiological Stress, Joshua Noah Ankeny

Graduate Theses, Dissertations, and Problem Reports

Unconventional Oil and Gas (UOG) production has been steadily expanding throughout the mid-Atlantic since 2008. Increased sedimentation, degraded water chemistry and an overall decrease in habitat quality due to UOG is anticipated to negatively impact aquatic inhabitants, a common observation in other stressed landscapes (i.e., mining, agriculture, development). We assessed stream health through both community analysis and physiological parameters (i.e., growth and hematocrit). A before-after-control-impact study uncovered three fish metrics that were significantly different following UOG disturbances. The invertivore-piscivore metric decreased following UOG disturbance (p = 0.045) whereas two benthic metrics saw a surprising increase (p = 0.003 and p …

Thermal Performance Of Growth At Consumption Maximum (C-Max) And Routine Metabolic Rate (Rmr) In Brook Trout Salvelinus Fontinalis From Four Populations In Central Appalachia, Cory Michael Hartman

Graduate Theses, Dissertations, and Problem Reports

Growth of an individual is the end result of many physiological processes stemming from the consumption of food items. These processes can be influenced by many variables including temperature. Current bioenergetics models for Brook Trout Salvelinus fontinalis suggest growth increases with temperature until an inflection point of 20.2°C. With a warming climate, and water temperatures expected to rise, the ability of a population to adapt to the warming environment is crucial to the survival of the species. For this study we sought to see how spatial variation would influence the specific consumption, conversion efficiency, and specific growth at consumption maximum …

Improving Broiler Performance Utilizing Modern Feed Additives, Niles Rourke Ridgeway

Graduate Theses, Dissertations, and Problem Reports

Modern broiler production strives to make modest improvements regarding broiler performance. This remains a goal as global population increases thusly increasing the quantity of an affordable, high quality source of protein. In recent years, an added stressor to achieving prior goals is mostly related to consciousness of environmental and consumer health. For decades, integrators have utilized minimal amounts of antibiotics as a barrier to most pathogens affecting the microbiome of a broiler’s gastrointestinal tract. Regulatory efforts have now prohibited most of previous sub-therapeutic medicine and started an in-depth scope of broiler gut health and interaction with antibiotic alternatives. In an …

Service Dogs For Veterans With Ptsd: Taxonomy, Work Stress Reduction, And Matching, Lindsay Parenti

Graduate Theses, Dissertations, and Problem Reports

Research suggests that many veterans with post-traumatic stress disorder (PTSD) struggle with reintegration (Sayer et al., 2010), but are unlikely to seek help or complete treatment (Schottenbauer et al., 2008). To make matters worse, available treatment options are often time consuming, challenging, and/or associated with negative side effects (Carafano & Hutchinson, 2017). Using animals as a treatment modality for veterans with PTSD is an emerging topic of interest and has shown promise (Owen, et al., 2016; Richie et al., 2016). However, several factors have hindered the advancement of this field. Obstacles include a lack of standard terminology and classification system, …

The Effect Of Feed Manufacture Techniques On Pathogen Reduction And Feed Nutrient Value, Timothy Paul Boltz

Graduate Theses, Dissertations, and Problem Reports

Experiments were conducted to evaluate how differing feed manufacture techniques, such as varying conditioning times and new equipment, can affect pathogen mitigation, feed manufacture metrics, amino acid digestibility, and enzyme recovery in feed. Feed hygienics are of increasing importance in providing safe feed to animals, and ultimately safe food for consumers. Salmonella has been identified as a major microbial hazard in animal feed that has been linked to animal and human illness. In chapter 2, the effects of standard pelleting and more thermally aggressive techniques were utilized to determine pathogen mitigation potential. Use of antibiotics has decreased in recent years …

Distribution And Habitat Use Of Benthic Fishes In The Lower Kanawha River, West Virginia, Nathaniel V. Owens

Graduate Theses, Dissertations, and Problem Reports

The Kanawha River in West Virginia is a historically diverse system in terms of ichthyofauna although, like many other large rivers, it has been subjected to a suite of anthropomorphic perturbations. These include this system being modified to allow the passage of commercial vessels for industry thus altering flow regimes, increased sedimentation, water depth, and channelization, and decreased interactions with its floodplain. Additionally, the fishes of this system have suffered from deleterious historical water quality issues caused by intensive timber operations, mining, and industrial waste. Post Clean Air Act, Clean Water Act, and Surface Mining Control and Reclamation Act water …

Ground Ingredients: Analysis Of Lead Exposure In The California Condor’S (Gymnogyps Californianus) Ground Foraging Habitat, Evan Michael Mcwreath

Graduate Theses, Dissertations, and Problem Reports

The California Condor (Gymnogyps californianus) is a critically endangered species that reached its nadir in 1987 with a population of 27 birds in the wild. Intensive management efforts have been implemented to aid the condors’ recovery, however, anthropogenic factors, like use of lead ammunition, continue to cause fatalities in this vulnerable population. Lead toxicosis, which is responsible for approximately 40% of all condor deaths since 1992, is one of the most significant threats to condors. In birds lead poisoning leads to neurological dysfunction, reproductive impairment, immune suppression, gastrointestinal disturbance, anemia, and ultimately increased vulnerability to predation, starvation, and infection. For …

Comparing The Effects Of Swimming Exercise And Dissolved Oxygen On Important Performance Parameters Of Early-Rearing Atlantic Salmon Salmo Salar And Rainbow Trout Oncorhynchus Mykiss., Thomas B. Waldrop

Graduate Theses, Dissertations, and Problem Reports

Swimming exercise (bodylenghts/sec), and dissolved oxygen (DO), are important environmental variables in aquaculture. While there is an obvious physiological association between these two parameters, their interaction has not been adequately studied in Atlantic salmon, Salmo salar and Rainbow trout, Oncorhynchus mykiss. Since both variables can be easily manipulated in modern aquaculture systems, we sought to assess the impact of these parameters, alone and in combination, on the performance, health, and welfare parameters of the two species. Atlantic salmon and Rainbow trout were stocked into twelve circular 0.5 m³ tanks and exposed to either high (1.5-2 BL/s) or low …

Lead Exposure Of North American Raptors, Vincent A. Slabe

Graduate Theses, Dissertations, and Problem Reports

Lead is an anthropogenic threat to terrestrial wildlife, has no physiological benefits, and is considered a neurotoxin. Legislation adopted in many developed countries restricts the uses of lead in consumer and industrial products such as pipe fittings, petrol, paint, and shot for use in waterfowl hunting. However, lead is currently categorized by the United States (US) government as a critical metal and common uses of lead still persist in North America. These include the use of rifle ammunition, shot in upland gamebird hunting, and fishing sinkers. Lead is also a by-product of multiple industrial operations including smelters, coal power plants, …