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Full-Text Articles in Life Sciences
Use Of Antibacterial Agents To Elucidate The Etiology Of Juvenile Oyster Disease (Jod) In Crassostrea Virginica And Numerical Dominance Of An Α- Proteobacterium In Jod-Affected Animals, Katherine J. Boettcher, Bruce J. Barber, John T. Singer
Use Of Antibacterial Agents To Elucidate The Etiology Of Juvenile Oyster Disease (Jod) In Crassostrea Virginica And Numerical Dominance Of An Α- Proteobacterium In Jod-Affected Animals, Katherine J. Boettcher, Bruce J. Barber, John T. Singer
Biology and Ecology Faculty Scholarship
Since 1988, juvenile oyster disease (JOD) has resulted in high seasonal losses of cultured Eastern oysters (Crassostrea virginica) in the Northeast. Although the cause of JOD remains unknown, most evidence is consistent with either a bacterial or a protistan etiology. For the purpose of discerning between these hypotheses, the antibacterial antibiotics norfloxacin and sulfadimethoxine-ormetoprim (Romet-B) were tested for the ability to delay the onset of JOD mortality and/or reduce the JOD mortality of cultured juvenile C. virginica. Hatchery-produced C. virginica seed were exposed in triplicate groups of 3,000 animals each to either norfloxacin, sulfadimethoxine-ormetoprim, or filter-sterilized seawater (FSSW) and deployed …
Multiplex Reverse Transcriptase Pcr Assay For Simultaneous Detection Of Three Fish Viruses, K. Williams, S. Blake, A. Sweeney, J. T. Singer, B. L. Nicholson
Multiplex Reverse Transcriptase Pcr Assay For Simultaneous Detection Of Three Fish Viruses, K. Williams, S. Blake, A. Sweeney, J. T. Singer, B. L. Nicholson
Biology and Ecology Faculty Scholarship
A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.