Life Sciences Commons^™

Basal Diet Fed To Recipient Mice Was The Driving Factor For Colitis And Colon Tumorigenesis, Despite Fecal Microbiota Transfer From Mice With Severe Or Mild Disease, Daphne Michelle Rodriguez Jimenez, Korry J. Hintze, Giovanni Rompato, Eliza C. Stewart, Abbey H. Barton, Emily Mortensen-Curtis, Porter A. Green, Arnaud J. Van Wettere, Aaron J. Thomas, Abby D. Benninghoff

Animal, Dairy, and Veterinary Science Faculty Publications

Consumption of the total Western diet (TWD) in mice has been shown to increase gut inflammation, promote colon tumorigenesis, and alter fecal microbiome composition when compared to mice fed a healthy diet, i.e., AIN93G (AIN). However, it is unclear whether the gut microbiome contributes directly to colitis-associated CRC in this model. The objective of this study was to determine whether dynamic fecal microbiota transfer (FMT) from donor mice fed either the AIN basal diet or the TWD would alter colitis symptoms or colitis-associated CRC in recipient mice, which were fed either the AIN diet or the TWD, using a 2 …

Fecal Microbiota Transfer (Fmt) From Tumor-Bearing Mice Fed The Total Western Diet (Twd) Does Not Contribute To A Higher Tumor Burden In Mice Fed A Healthy Diet, Porter Green

Student Research Symposium

Colorectal cancer (CRC) is the third most common cause of cancer death in the United States. Diet and gut microbiome have shown to influence the development of colitis associated colorectal cancer (CAC). For this study, the goal was to determine the association between colon inflammation, diet and microbiome in the development of CAC in mice. The primary objective of this study was to determine the contribution of gut microbiota from mice donors who have previously consumed one of two basal diets: 1) the standard AIN93G diet, which is designed to promote rodent health; and 2) the total Western diet (TWD), …

Consumption Of The Total Western Diet Promotes Colitis And Inflammation-Associated Colorectal Cancer In Mice, Abby D. Benninghoff, Korry J. Hintze, Stephany P. Monsanto, Daphne Michelle Rodriguez Jimenez, Ashli H. Hunter, Sumira Phatak, James J. Pestka, Arnaud J. Van Wettere, Robert E. Ward

Animal, Dairy, and Veterinary Science Faculty Publications

Consumption of a Western type diet is a known risk factor for colorectal cancer. Our group previously developed the total Western diet (TWD) for rodents with energy and nutrient profiles that emulate a typical Western diet. In this study, we tested the hypothesis that consumption of the TWD would enhance colitis, delay recovery from gut injury and promote colon tumorigenesis. In multiple experiments using the azoxymethane + dextran sodium sulfate or Apc^Min/+ mouse models of colitis-associated colorectal carcinogenesis (CAC), we determined that mice fed TWD experienced more severe and more prolonged colitis compared to their counterparts fed the standard …

Can Adding Black Raspberries To The Western Diet Reduce Factors That Lead To Colorectal Cancer?, Tess Armbrust, Canyon Neal

Research on Capitol Hill

• Americans with leaky gut, or increased intestinal permeability, often develop chronic inflammation (colitis) and are at a greater risk for developing colorectal cancer (CRC).
• A poor diet, high in processed foods but low in fruits and vegetables, is another notable risk factor for CRC.
• Micronutrient supplementation reduces colitis and prevents progression to colitis associated colorectal cancer (CAC).
• Due to their high concentration of anthocyanins, black raspberries have demonstrated protective effects against inflammation in the body.
• Certain antibodies are indicative of inflammation which leads to CRC.