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Factors Affecting Skeletal Muscle Protein Synthesis In The Horse, Ashley Leigh Wagner

Theses and Dissertations--Animal and Food Sciences

Skeletal muscle protein synthesis is regulated by the mammalian target of rapamycin (mTOR) signaling pathway. The first objective was to optimize the methodological procedures for assessing mTOR signaling in horses. The response of mTOR signaling (P-Akt Ser⁴⁷³, P-S6K1 Thr³⁸⁹, P-rpS6 Ser^{235/26 & 240/244}, and P-4EBP1 Thr^37/46 by Western blotting techniques) to meal consumption was determined at three gluteal muscle biopsy depths (6, 8, and 10 cm), and the repeatability of the contralateral side at 8 cm during 5 days of repeated biopsies. There was no effect (P > 0.05) of sampling side or biopsy …