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Effects Of Microtubule Stabilizing And Recovery Agents On Vitrified Bovine Oocytes, Emily Dzekunskas
Effects Of Microtubule Stabilizing And Recovery Agents On Vitrified Bovine Oocytes, Emily Dzekunskas
LSU Master's Theses
Oocyte vitrification, while beneficial for research and species conservation, has limited success due to cryoinjury to the meiotic spindle. This study aimed to evaluate treatments to improve meiotic spindle recovery after vitrification by treating bovine oocytes with either a microtubule stabilizing agent, or a microtubule recovery agent. In the first two experiments, Taxol and epothilone B were used to treat bovine oocytes before vitrification. Both compounds have microtubule stabilizing properties and are known antimitotic compounds commonly used in cancer treatment. Oocytes were incubated with one of the microtubule stabilizing agents for 15 minutes before vitrification at one of three concentrations …