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Animal Sciences

Iowa State University

1990

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Comparison Of Homogenization By Blending Or Stomaching On The Recovery Of Listeria Monocytogenes From Beef Tissues, James S. Dickson Jan 1990

Comparison Of Homogenization By Blending Or Stomaching On The Recovery Of Listeria Monocytogenes From Beef Tissues, James S. Dickson

James S. Dickson

Homogenization by blending or stomaching was compared for the recovery of Listeria monocytogenes from inoculated intact beef tissue. There were no differences in numbers of recovered bacteria (P > 0.10) attributable to either homogenization time or method. Fewer viable bacteria (PcO.05) were recovered in phosphate buffer than either buffered peptone water or 2% trisodium citrate buffer. Tween 80 increased the numbers of bacteria recovered from fat tissue (PcO.05). Stomaching is an acceptable method for homogenizing samples for Listeria analysis.