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Full-Text Articles in Life Sciences
Molecular Phylogeny Of Cave Dwelling Eremogryllodes Crickets (Orthoptera, Myrmecophilidae) Across Zagros Mountains And Southern Iran, Mohadeseh Sadat Tahami, Mina Hojat-Ansari, Anna Namyatova, Saber Sadeghi
Molecular Phylogeny Of Cave Dwelling Eremogryllodes Crickets (Orthoptera, Myrmecophilidae) Across Zagros Mountains And Southern Iran, Mohadeseh Sadat Tahami, Mina Hojat-Ansari, Anna Namyatova, Saber Sadeghi
International Journal of Speleology
Recently, several new species and subspecies from the genus Eremogryllodes Chopard, 1929 (Insecta: Orthoptera: Myrmecophilidae) inhabiting caves of Iran, have been described based on morphology. The high variation of genitalia structure along with high similarity of external morphology between populations hamper the precise species identification. Thus, molecular approaches are critical to determine the taxonomic positions of species/subspecies of this genus. Here we provide the molecular phylogeny, based on the 16S rRNA mitochondrial gene, of recently described species of Eremogryllodes along with some unidentified specimens from the same region. The results support the monophyly of the family Myrmecophilidae. The topology of …
Investigating The Development Of Fecal Bacterial Communities In Growing Dairy Calves, Emily Fowler
Investigating The Development Of Fecal Bacterial Communities In Growing Dairy Calves, Emily Fowler
Electronic Theses and Dissertations
The gut development of young calves is crucial for the producer and the calf's future. Many factors can affect the development of a young calf's gastrointestinal system, and there has been little research into the fecal microbiome development of calves from 0 to 12 weeks of age. To gain further insight into this process, development of the fecal microbiome in 12 dairy calves was investigated. Fecal bacterial composition was determined at four time points (weeks 0, 4, 8 and 12) using the 16S rRNA gene through PCR-amplification of the V1-V3 regions from fecal microbial genomic DNA, followed by Illumina MiSeq …
Investigation Of Sulfur Cycling In Marine Sponge Cinachyrella Spp. From A South Florida Reef, Shelby K. Cain
Investigation Of Sulfur Cycling In Marine Sponge Cinachyrella Spp. From A South Florida Reef, Shelby K. Cain
All HCAS Student Capstones, Theses, and Dissertations
Symbionts within marine sponges are actively participating in the biogeochemical cycles. Among them, the role of symbiont microbes in the sulfur cycle remains a mystery. This study measured the abundance of microbes within the genus Cinachyrella before and after exposure to hydrogen sulfide. A four-part study was conducted: a) five-hour drop experiments, b) vertical distribution experiments, c) five-hour uptake experiments, and d) long-term exposure experiments. The five-hour drop experiment utilized a microsensor to measure sulfide levels, which was lowered 1.0 mm every thirty minutes for a total of 5 hours. Three trials were performed, each with one sponge and a …
A Phylogenetic Analysis Of Mycoplasma Strains Circulating In Sheep Pneumonia In Thekars Region Of Turkey, Olcay Öztürkler, Sali̇h Otlu
A Phylogenetic Analysis Of Mycoplasma Strains Circulating In Sheep Pneumonia In Thekars Region Of Turkey, Olcay Öztürkler, Sali̇h Otlu
Turkish Journal of Veterinary & Animal Sciences
This study aimed to investigate Mycoplasma species by cultural and molecular methods in 250 pneumonics and 30 healthy appearance sheep lungs brought from slaughterhouse and butcheries in the Kars region of Turkey. The phylogenetic positioning of the bacterial strains to be obtained in the present study was another remarkable outcome of the study. Mycoplasma isolation was achieved solely in 26 (10.4%) of 250 sheep lungs with pneumonia. Mycoplasma ovipneumoniae was found predominant species in 12 (46.15%) of samples and that was followed by Mycoplasma arginini in 4 (15.38%) of strains. Moreover, two samples were found to be co-infected with the …
A Comparative Study Of Equine Gut Microbiomes Using 16s And 18s Rrna Sequencing, Jillian Jorgenson
A Comparative Study Of Equine Gut Microbiomes Using 16s And 18s Rrna Sequencing, Jillian Jorgenson
Undergraduate Honors Thesis Projects
The gut microbiome of domestic horses (Equus ferus caballus) can be broken down into two primary divisions of interest: (1) prokaryotic composition, consisting of bacteria and archaea and (2) fungal composition. Within the gut, there are also eukaryotic parasite communities that may interact with the microbiome. While each has been the subject of various scientific explorations, in this study we analyze the potential interconnectedness of these categories. To determine the extent to which each component of the gut microbiome/parasitic communities influences the other, DNA was extracted and amplified from the fecal samples of eight horses. A combination of …
The Effect Of Regular Or Reduced-Fat Distillers Grains With Solubles On Rumen Methanogenesis And The Rumen Bacterial Community, E. Castillo-Lopez, C. J. R. Jenkins, N. D. Aluthge, W. Tom, P. J. Kononoff, S. C. Fernando
The Effect Of Regular Or Reduced-Fat Distillers Grains With Solubles On Rumen Methanogenesis And The Rumen Bacterial Community, E. Castillo-Lopez, C. J. R. Jenkins, N. D. Aluthge, W. Tom, P. J. Kononoff, S. C. Fernando
Department of Animal Science: Faculty Publications
Aims: The effect of feeding dried distillers grains with solubles (DDGS) or reduced-fat DDGS (RFDG) on ruminal methanogenesis and the rumen bacterial community of dairy cattle was evaluated. Methods and Results: Treatments were CONT, a diet with no distillers grains; DG, inclusion of 20% DDGS; rfDG, inclusion of 20% RFDG; and MIX, inclusion of 10% DDGS and 10% RFDG. Methane emission was measured; rumen bacterial community was evaluated by sequencing the V4 region of the 16S rRNA gene. Total methane production remained unaffected. However, feeding distillers grains tended to reduce methanogenesis per unit of feed intake, decreased the abundance of …
Taxonomic Status Of A Newly Described Island Population Of The Smooth Newtlissotriton Vulgaris (Linnaeus, 1758) From Bozcaada (Çanakkale, Turkey), Nurşen Çördük, Çi̇ğdem Gül, Murat Tosunoğlu, Konstantinos Sotiropoulos
Taxonomic Status Of A Newly Described Island Population Of The Smooth Newtlissotriton Vulgaris (Linnaeus, 1758) From Bozcaada (Çanakkale, Turkey), Nurşen Çördük, Çi̇ğdem Gül, Murat Tosunoğlu, Konstantinos Sotiropoulos
Turkish Journal of Zoology
The taxonomic status and phylogenetic position of the recently recorded smooth newt (Lissotriton vulgaris) population from the island of Bozcaada (Çanakkale, Turkey) is clarified on the basis of morphological and molecular phylogenetic analyses. The L. vulgaris population from Bozcaada presents body proportions and morphological features of subsp. schmidtlerorum, such as small body length, absence of the tail filament, and dorsal crest with pointed free margin. Similarly, phylogenetic analyses of mitochondrial DNA sequences (ND4, 16S rRNA) place the Bozcaada population within Clade E of the recent L. vulgaris phylogeny, which consists of L. v. schmidtlerorum populations.
Comparative Evaluation Of Sequence Analysis Of 16s Rrna And Rpobgenes For Identification Of Aquatic Mycobacteria, Fazel Pourahmad, Randolph Harvey Richards
Comparative Evaluation Of Sequence Analysis Of 16s Rrna And Rpobgenes For Identification Of Aquatic Mycobacteria, Fazel Pourahmad, Randolph Harvey Richards
Turkish Journal of Veterinary & Animal Sciences
The nucleotide sequences of partial 16S rRNA and bacterial RNA polymerase ß-subunit (rpoB) genes for 57 mycobacterial strains were determined. Compared to the 16S rRNA gene sequences, variable regions were scattered along the whole fragment sequence, indicating that the rpoB gene is more polymorphic. Unlike 16S rRNA sequences, species variation was observed within M. fortuitum strains. The topology of the rpoB-based phylogenetic tree was almost the same as that of the 16S rRNA sequence analysis. These results suggest that the rpoB gene is a highly conserved gene, and taxonomical studies based on this gene may be comparable with similar studies …
Molecular Systematic Analyses Of Mediterranean Skates (Rajiformes), Cemal Turan
Molecular Systematic Analyses Of Mediterranean Skates (Rajiformes), Cemal Turan
Turkish Journal of Zoology
Mitochondrial 16S ribosomal RNA was used to elucidate the pattern of relationships and systematic status of 4 genera, including 9 species of skates (Rostroraja alba, Leucoraja fullonica, Dipturus oxyrinchus, D. batis, Raja clavata, R. montagui, R. asterias, R. miraletus, and R. radula), living in the Mediterranean and Black Seas. The 16S rDNA dataset contained 39 variable and 29 parsimony informative sites. The mean nucleotide diversity (Pi) was 0.018. The genetic distances between pairs of species showed genetic homogeneity between R. clavata and R. montagui. The highest value of sequence divergence was detected between D. oxyrinchus and R. asterias (0.040). Low …