Life Sciences Commons^™

Differential Expression Of Cyb5a In Chinese And European Pig Breeds Due To Genetic Variations In The Promoter Region..Pdf, Ying Bai, Jibin Zhang, Y Xue, Yl Peng, Gang Chen, Meiying Fang

Jibin Zhang

Effects Of Corn Crop Residue Grazing On Soil Physical Properties And Subsequent Soybean Production In A Corn- Soybean Crop Rotation (A Progress Report), Justin Clark, Jim Russell, Douglas Karlen, Darrell Busby, L. James Secor, Brian Peterson, Larry Pellack, Carroll Olsen, Shawn Shouse

Douglas L Karlen

Beginning in 1999, two locations in Iowa (Chariton, Atlantic) were used to study the effects of corn residue grazing by beef cows on soil characteristics and soybean yields the following growing season. Cows were allowed to graze inside selected paddocks at monthly periods throughout the fall and winter. For a grazed and ungrazed comparison, grazing exclosures were used inside the grazed paddocks while one paddock was left ungrazed for a control. The use of this design was to determine if grazing had any adverse effects on soil characteristics and at what date and weather conditions did they occur. Also equal …

Effects Of Corn Crop Residue Grazing On Soil Physical Properties And Subsequent Soybean Production In A Corn-Soybean Crop Rotation (A Progress Report), Justin Clark, James R. Russell, Douglas Karlen, Darrell Busby, L. James Secor, Brian Peterson, Larry Pellack, Carroll Olsen, Shawn C. Shouse

Douglas L Karlen

The highest cost to beef cow-calf producers is the feeding of stored feeds in winter months. To lower feed costs, many producers will try to extend the grazing season into the winter. The primary resource for winter grazing in the Midwest is corn crop residues. On the average, corn crop residue grazing will reduce the amount of hay needed to maintain cows by approximately one-half ton per acre grazed over the winter. Although crop residue grazing is quite effective in reducing feed costs, some producers are concerned that corn residue grazing will have an adverse effect on soybean yields the …

Effects Of Corn Crop Residue Grazing On Soil Physical Properties And Subsequent Soybean Production In A Corn–Soybean Crop Rotation (A Progress Report), Justin Clark, James R. Russell, Douglas Karlen, Darrell Busby, L. James Secor, Brian Peterson, Larry Pellack, Carroll Olson, Dallas L. Maxwell, Shawn C. Shouse

Douglas L Karlen

Beginning in 1999, two locations in Iowa (Chariton, Atlantic) were used to study the effects of corn residue grazing by beef cows on soil characteristics and soybean yields the following growing season. Cows were allowed to graze inside selected paddocks at monthly periods throughout the fall and winter. For a grazed and ungrazed comparison, grazing exclosures were used inside the grazed paddocks, while one paddock was left ungrazed for a control. Also, the following year, equal portions of the fields went to no-tillage and disked soil prior to soybean planting so that effects of corn residue grazing on tillage treatments …

Winter Grazing Of Corn Residues: Effects On Soil Properties And Subsequent Crop Yields From A Corn-Soybean Crop Rotation, W. Darrell Busby, James R. Russell, Douglas L. Karlen, L. James Secor, Brian Peterson, Carroll Olson, Shawn C. Shouse, Dallas L. Maxwell

Douglas L Karlen

Corn residues could be a good resource for winter cattle grazing. The study investigates whether winter grazing causes soil compaction and yield reduction in crops that are planted following grazing.

Effects Of Corn Crop Residue Grazing On Soil Physical Properties And Subsequent Soybean Production In A Corn–Soybean Crop Rotation, Justin Clark, James R. Russell, Douglas Karlen, W. Darrell Busby, Brian Peterson, Larry Pellack, Dallas L. Maxwell

Douglas L Karlen

For three years beginning in 1999, a 96-acre field near Atlantic, Iowa was used to study the effects of corn residue grazing by beef cows on soil characteristics and soybean yields in subsequent years. Each winter, cows were allowed to graze corn crop residues inside selected paddocks in four sub-fields over five monthly periods. To compare the effects of grazing, one paddock was left as an ungrazed control. At the end of grazing in the spring, soil bulk density, moisture content, and penetration resistance were measured inside and 15 ft outside twelve grazing exclosures in each paddock. Soil surface roughness, …

Birds Of The Rocky Mountains—Species Accounts, Pages 242–261: Flycatchers & Larks, Paul A. Johnsgard

Paul Johnsgard

Olive-sided Flycatcher (Contopus borealis) Western Wood"pewee (Contopus sordidulus) Yellow"bellied Flycatcher (Empidonax flaviventris) Alder Flycatcher (Empidonax alnorum) Willow Flycatbher (Empidonax trailli) Least Flycatcher (Empidonax minimus) Hammond's Flycatcher (Empidonax hammondi) Dusky Flycatcher (Empidonax oberholseri) Gray Flycatcher (Empidonax wrightii) Western Flycatcher (Empidonax difficilis) Eastern Phoebe (Sayornis phoebe) Say's Phoebe (Sayornis saya) Vermilion Flycatcher (Pyrocephalus rubinus) Ash-throated Flycatcher (Myiarchus cinerascens) Cassin's Kingbird (Tyrannus vociferus) Western Kingbird (Tyrannus verticalis) Eastern Kingbird (Tyrannus tyrannus) Scissor-tailed Flycatcher (Muscivora forficata) Horned Lark (Eremophila alpestris)

Evaluation Of Animal Model Research, Kenneth J. Shapiro

Kenneth J. Shapiro, PhD

It is argued that a concept of evaluation of animal models that is broader and more useful than validation is available. Productive generativity refers to the degree to which a model furthers understanding and leads to more-effective treatment interventions. Results of the application of this novel evaluative frame to several animal models of eating disorders show that this animal-based research has not been productive. The question of the relation between clinic and animal laboratory is discussed.

Detection Of Panulirus Argus Virus 1 In Caribbean Spiny Lobsters, Megan M. Montgomery-Fullerton, Roland Cooper, Kathryn M. Kauffman, Jeffrey D. Shields, Robert E. Ratzlaff

Roland A. Cooper

Panulirus argus Virus 1 (PaV1) is a pathogenic virus that infects Caribbean spiny lobsters P. argus in the Florida Keys. We have developed a PCR detection assay for PaV1 for the purpose of studying the natural history of the virus and for monitoring the prevalence of infection. The detection of the virus in hemolymph and other tissues is based on the PCR amplification of a 499 bp product using specific primers designed from a cloned fragment of the PaV1 genome. The sensitivity limit for the assay was 1.2 fg of purified viral DNA. The PaV1 primers did not react with …

Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Caiwen Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Carmony L. Hartwig, Roland A. Cooper, Robert E. Ratzlaff

Roland A. Cooper

Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and …

Burrow Morphology And Behavior Of The Mud Shrimp Upogebia Omissa (Decapoda: Thalassinidea: Upogebiidae), Vania Rodrigues Coelho, Roland Arthur Cooper, Sergio De Almeida Rodrigues

Roland A. Cooper

The burrow morphology, burrowing behavior and feeding mechanisms of the thalassinidean shrimp Upogebia omissa were studied. Twenty burrow casts were made in situ with epoxy resin, and an overall 'Y' shape was most frequently observed. Several burrows consisted of a single, oblique tunnel; burrow diameter was positively correlated with burrow length, maximum depth and distance between openings. Additionally, burrow length was positively associated with maximum depth, indicating that as burrow length increased burrow depth increased; i.e. burrows spread vertically rather than horizontally. Total sediment displacement by the burrows accounted for 2.6 % of sediment to a depth of 30 cm. …

Effect Of Biofield Treated Energized Water On The Growth And Health Status In Chicken (Gallus Gallus Domesticus), Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Mahendra Kumar Trivedi

Tb181: 2000 Milk Processing Costs In Maine, Timothy J. Dalton, George K. Criner, John Halloran

George K. Criner

The objective of this study is to provide an estimate of the theoretically lowest achievable costs of processing and distributing milk in Maine. This processing and distribution margin (referred hence as "margin") is estimated for a state-of-the-art processing plant assumed to be located in the Portland, Maine, area. The plant is assumed to produce and distribute a line of products including white milk, chocolate milk, orange juice, and other fruit drinks, and to distribute additional purchased products such as cheeses and yogurts. This report will present information for four plants to better study the impact of plant size and production …

B853: Cost Of Producing Milk In Maine: Results From The 2010 Dairy Cost Of Production Survey, Richard Kersbergen, Gary Anderson, George Criner, Anthony Davis

George K. Criner

The present study reports on a survey conducted for the Maine Milk Commission to determine the cost of milk production for 2010. This assessment was undertaken sooner than the normal three-year update because of the difficult economic climate for dairy farms in Maine.

Tb204: Organic Milk Production In Maine: Attributes, Costs, And Returns, Amelia L. Cook, Patrick S. Heacock, George K. Criner, Lisa A. Bragg

George K. Criner

This report summarizes attributes, costs, and returns for organic dairy farms in Maine that responded to the 2008 dairy cost of production survey. This survey and analysis was conducted by the University of Maine in cooperation with the Maine Milk Commission. This publication reports on data collected over the 2007 production year. Analysis and discussion of the data revolve around four categories. The first category averages all 30 organic farms to create a statewide group. The authors then broke these 30 farms into three size groups (small, medium, and large) based on the number of cows on each farm. There …

The Shellfish Corner: Filter Feeding Bivalves As Processors Of Coastal Waters, Michael Rice

Michael A Rice

Bivalves have a profound role in controlling the boom and bust cycles of seasonal phytoplankton blooms, and the increased rates of sediment deposition to the bottom by bivalves are an important "coupler" between the water column and the bottom that stimulates the rate of decomposition and other processes in the sediments.

Pain In Aquatic Animals, Lynne U. Sneddon

Lynne Sneddon, PhD

Recent developments in the study of pain in animals have demonstrated the potential for pain perception in a variety of wholly aquatic species such as molluscs, crustaceans and fish. This allows us to gain insight into how the ecological pressures and differential life history of living in a watery medium can yield novel data that inform the comparative physiology and evolution of pain. Nociception is the simple detection of potentially painful stimuli usually accompanied by a reflex withdrawal response, and nociceptors have been found in aquatic invertebrates such as the sea slug Aplysia. It would seem adaptive to have a …

What Do Zebrafish Want? Impact Of Social Grouping, Dominance And Gender On Preference For Enrichment, Paul Schroeder, Soffia Jones, Iain S. Young, Lynne U. Sneddon

Lynne Sneddon, PhD

Although environmental enrichment is known to improve laboratory rodent wellbeing and enhance scientific data collection, relatively little is known with regards to the type of enrichment that might be useful for zebrafish (Danio rerio). Therefore, this study explored if zebrafish displayed preferences for a range of enrichments, including substrates, artificial plants, combinations thereof and airstones. Tanks divided into two compartments containing different enrichment cues were used to determine the preferences of zebrafish housed in pairs and groups of eight. When comparing time spent in enriched versus barren compartments, dominant individuals in a pair displayed a preference for substrate and behaviourally …

Investigation Of Van Gogh-Like 2 Mrna Regulation And Localisation In Response To Nociception In The Brain Of Adult Common Carp (Cyprinus Carpio), Siobhan C. Reilly, Anja Kipar, David J. Hughes, John P. Quinn, Andrew R. Cossins, Lynne U. Sneddon

Lynne Sneddon, PhD

The Van Gogh-like 2 (vangl2) gene is typically associated with planar cell polarity pathways, which is essential for correct orientation of epithelial cells during development. The encoded protein of this gene is a transmembrane protein and is highly conserved through evolution. Van Gogh-like 2 was selected for further study on the basis of consistent regulation after a nociceptive stimulus in adult common carp and rainbow trout in a microarray study. An in situ hybridisation was conducted in the brain of mature common carp (Cyprinus carpio), 1.5 and 3 h after a nociceptive stimulus comprising of an acetic acid injection to …

Three Rs Approaches In The Production And Quality Control Of Fish Vaccines, Paul J. Midtlyng, Coenraad F.M. Hendriksen, Elisabeth Balks, Lukas Bruckner, Lawrence Elsken, Øystein Evensen, Kjetil Fyrand, Allison Guy, Marlies Halder, Penny Hawkins, Gunn Kisen, Anne Berit Romstad, Kira Salonius, Patrick Smith, Lynne U. Sneddon

Lynne Sneddon, PhD

The workshop on Three Rs Approaches in the Production and Quality Control of Fish Vaccines aimed a) to identify animal tests currently stipulated for the production and quality control of fish vaccines and to highlight animal welfare concerns associated with these tests; b) to identify viable options to replace, reduce, and refine animal use for fish vaccine testing; and c) to discuss the way forward and set out how the Three Rs may be implemented without jeopardizing the quality of the vaccines. The workshop participants -- experts from academia, regulatory authorities, a scientific animal welfare organization, and the fish vaccine …

Pain Perception In Fish: Evidence And Implications For The Use Of Fish, Lynne U. Sneddon

Lynne Sneddon, PhD

Pain assessment in fish is particularly challenging due to their evolutionary distance from humans, their lack of audible vocalization, and apparently expressionless demeanour. However, there are criteria that can be used to gauge whether pain perception occurs using carefully executed scientific approaches. Here, the standards for pain in fish are discussed and can be considered in three ways: neural detection and processing of pain; adverse responses to pain; and consciously experiencing pain. Many procedures that we subject fish to cause tissue damage and may give rise to the sensation of pain. Fish are popular as pets, in animal exhibits, and …

Environmental Change Alters Personality In The Rainbow Trout, Oncorhynchus Mykiss, Ashley J. Frost, Jack S. Thomson, Charlotte Smith, Hannah C. Burton, Ben Davis, Phillip C. Watts, Lynne U. Sneddon

Lynne Sneddon, PhD

Boldness is a personality trait that defines how individuals respond to risky situations and has clear fitness consequences. Since the adaptive value of boldness is context dependent, the benefit of a distinct personality is less clear when the environment is unpredictable. An ability to modulate behaviour can be beneficial, although as behavioural plasticity itself may be costly this depends on the levels of environmental stability. Both boldness and its plasticity are linked with physiological stress coping mechanisms, whereby animals with reduced glucocorticoid responses to stress are bolder and less flexible in behaviour. We investigated the behavioural changes made by bold …

Phytogenic Feed Additives As An Alternative To Antibiotic Growth Promoters In Broiler Chickens, G. R. Murugesan, Basharat Syed, Sudipto Haldar, Chasity Pender

G. R. Murugesan

The recent trend toward reduction of antibiotic growth promoters (AGP) in North American poultry diets has put tremendous pressure on the industry to look for viable alternatives. In this context, phytogenic feed additives (PFA) are researched to improve gut health and thereby performance. An experiment was conducted with the objective to evaluate the effects of PFA as an alternative to AGP on small intestinal histomorphology, cecal microbiota composition, nutrient digestibility, and growth performance in broiler chickens. A total of 432, day-old Vencobb 400 broiler chicks were randomly assigned to one of three dietary groups, each consisting of 12 replicate pens …

Establishing A National Shellfish Sanitation Program In The Gambia, West Africa, Michael A. Rice, Foday Conteh, Karen Kent, Brian Crawford, Banja Bamba, Fatou Janha, Ismaila Bojang

Michael A Rice

A successful national program to assure sanitary quality of molluscan shellfish requires a multi-disciplinary and multi-agency governmental training, data collection, policy development and management effort in collaboration with members of the shellfish industry. The Tanbi Wetlands and other estuaries of Gambia support shellfisheries for oysters, Crassostrea tulipa, and the senile ark, Senelia senilis, conducted by the TRY Oyster Women’s Association. With low shellfish prices and a small local market, a Gambian National Shellfish Sanitation Program (GNSSP) was begun as a means to boost consumer confidence and allow market access to Gambia’s robust seasonal international tourism trade. Gambian officials began training …

Characterisation Of Chemosensory Trigeminal Receptors In The Rainbow Trout, Oncorhynchus Mykiss: Responses To Chemical Irritants And Carbon Dioxide, Jessica J. Mettam, Catherine R. Mccrohan, Lynne U. Sneddon

Lynne Sneddon, PhD

Trigeminally innervated, mechanically sensitive chemoreceptors (M) were previously identified in rainbow trout, Oncorhynchus mykiss, but it is not known whether these receptors are responsive only to noxious, chemical irritants or have a general chemosensory function. This study aimed to characterise the stimulus–response properties of these receptors in comparison with polymodal nociceptors (P). Both P and M gave similar response profiles to acetic acid concentrations. The electrophysiological properties were similar between the two different afferent types. To determine whether the receptors have a nociceptive function, a range of chemical stimulants was applied to these receptors, including non-noxious stimuli such as ammonium …

Plasticity Of Boldness In Rainbow Trout, Oncorhynchus Mykiss: Do Hunger And Predation Influence Risk-Taking Behaviour?, Jack S. Thomson, Phillip C. Watts, Tom G. Pottinger, Lynne U. Sneddon

Lynne U. Sneddon, Ph.D.

Boldness, a measure of an individual's propensity for taking risks, is an important determinant of fitness but is not necessarily a fixed trait. Dependent upon an individual's state, and given certain contexts or challenges, individuals may be able to alter their inclination to be bold or shy in response. Furthermore, the degree to which individuals can modulate their behaviour has been linked with physiological responses to stress. Here we attempted to determine whether bold and shy rainbow trout, Oncorhynchus mykiss, can exhibit behavioural plasticity in response to changes in state (nutritional availability) and context (predation threat). Individual trout were initially …

Clinical Anesthesia And Analgesia In Fish, Lynne U. Sneddon

Lynne Sneddon, PhD

Fish have become a popular experimental model and companion animal, and are also farmed and caught for food. Thus, surgical and invasive procedures in this animal group are common, and this review will focus on the anesthesia and analgesia of fish. A variety of anesthetic agents are commonly applied to fish via immersion. Correct dosing can result in effective anesthesia for acute procedures as well as loss of consciousness for surgical interventions. Dose and anesthetic agent vary between species of fish and are further confounded by a variety of physiological parameters (e.g., body weight, physiological stress) as well as environmental …

The Efficacy Of Three Types Of Analgesic Drugs In Reducing Pain In The Rainbow Trout, Oncorhynchus Mykiss, Jessica J. Mettam, Lois J. Oulton, Catherine R. Mccrohan, Lynne U. Sneddon

Lynne Sneddon, PhD

Recent research has shown the possibility of pain perception in fish; therefore, the use of analgesia or “painkillers” should be considered for invasive procedures. However, there is relatively little information on the effectiveness of analgesic drugs nor on the appropriate dose for fish. This study assessed the efficacy of three types of drug: an opioid, buprenorphine, a non-steroidal anti-inflammatory drug (NSAID), carprofen and a local anaesthetic, lidocaine. Each drug was tested at three doses on rainbow trout that were noxiously stimulated and the most effective dose was also given to fish experiencing no pain to investigate side-effects. Ventilation rate and …

Diffusion Tensor Imaging Of Dolphin Brains Reveals Direct Auditory Pathway To Temporal Lobe, Gregory S. Berns, Peter F. Cook, Sean Foxley, Saad Jbabdi, Karla L. Miller, Lori Marino

Lori Marino, Ph.D.

The brains of odontocetes (toothed whales) look grossly different from their terrestrial relatives. Because of their adaptation to the aquatic environment and their reliance on echolocation, the odontocetes’ auditory system is both unique and crucial to their survival. Yet, scant data exist about the functional organization of the cetacean auditory system. A predominant hypothesis is that the primary auditory cortex lies in the suprasylvian gyrus along the vertex of the hemispheres, with this position induced by expansion of ‘associative0 regions in lateral and caudal directions. However, the precise location of the auditory cortex and its connections are still unknown. Here, …

Thinking Pigs: A Comparative Review Of Cognition, Emotion, And Personality In Sus Domesticus, Lori Marino, Christina M. Colvin

Lori Marino, PhD

While relatively little is known about the psychology of domestic pigs, what is known suggests that pigs are cognitively complex and share many traits with animals whom we consider intelligent. This paper reviews the scientific evidence for cognitive complexity in domestic pigs and, when appropriate, compares this literature with similar findings in other animals, focusing on some of the more compelling and cutting-edge research results. The goals of this paper are to: 1) frame pig cognition and psychology in a basic comparative context independent of the livestock production and management setting; and 2) identify areas of research with pigs that …