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The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and codingregions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Comparative analysis of the promoter region among 20 species including pig revealed four conserved regions that harbor transcriptional factors involved in adipose differentiation. Using a pooled DNA sequencing approach, we discovered 39 single nucleotide polymorphisms (SNPs) in the pig FTO gene and four of them were …

3β-hydroxysteroid dehydrogenases (HSDs) and 17βHSDs are pivotal enzymes involved in synthesis, activation and deactivation of steroid hormones. Therefore, they play an important role to maintain homeostasis of hormone and regulate the synthesis and metabolism of hormones, and they are also related to metabolism of androstenone which can cause boar taint in male pigs. Through ligation of different PCR fragments of these genes from Large White pigs to pGL3-basic plasmid and subsequent transient transfection of constructed vectors into HepG2 cell culture, we found that the fragment from -1038bp to 45bp in porcine 3βHSD gene and the fragment from -966bp to 39bp …