Life Sciences Commons^™

Reducing Equifinality Using Isotopes In A Process-Based Stream Nitrogen Model Highlights The Flux Of Algal Nitrogen From Agricultural Streams, William I. Ford, James F. Fox, Erik Pollock

Biosystems and Agricultural Engineering Faculty Publications

The fate of bioavailable nitrogen species transported through agricultural landscapes remains highly uncertain given complexities of measuring fluxes impacting the fluvial N cycle. We present and test a new numerical model named Technology for Removable Annual Nitrogen in Streams For Ecosystem Restoration (TRANSFER), which aims to reduce model uncertainty due to erroneous parameterization, i.e., equifinality, in stream nitrogen cycle assessment and quantify the significance of transient and permanent removal pathways. TRANSFER couples nitrogen elemental and stable isotope mass‐balance equations with existing hydrologic, hydraulic, sediment transport, algal biomass, and sediment organic …

Influence Of Media Composition On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus Utilized For Co2 Mitigation, Czarena L. Crofcheck, Xinyi E, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews

Biosystems and Agricultural Engineering Faculty Publications

Atmospheric carbon dioxide levels have increased since the industrial revolution due to increasing combustion of fossil fuels. One possible CO₂ mitigation strategy is the use of microalgae for mitigation of CO₂. This paper focuses on the influence of media composition on the growth rate of two microalgae strains, Chlorella vulgaris and Scenedesmus actus. A KNO₃ based medium was found to work better for Chlorella, while a urea based equivalent worked better for Scenedesmus. The urea based media investigated here resulted in growth similar to that found with previously reported KNO₃ based media. …

A Study Of Naturally Occurring Algicides Produced By Freshwater Algae, Denny O. Harris, Harry D. Caldwell

KWRRI Research Reports

The mode of action of the algicide produced by Pandorina morum was examined by exposing Volvox globator and isolates spinach chloroplasts to a partially purified algicide preparation. Oxygen evolution of Volvox, whole chloroplasts and broken chloroplasts (minus the Calvin cycle),was reduced indicating that the algicide inhibits the light reactions of photosynthesis. Oxygen evolution studies of other Volvocaceae confirmed the observation that Pandorina morum is not significantly influenced by its own inhibitor. Molecular weight approximation by gel filtration established that the inhibitor has a low molecular weight (probably below 100 mw). Field studies indicate that this algicide has tremendous potential as …

A Study Of Water-Soluble Inhibitory Compounds (Algicides) Produced By Fresh-Water Algae, Denny O. Harris, Manhar C. Parekh

KWRRI Research Reports

A complex system of growth inhibitors was observed in the green algae (Volvocaceae). Inhibitors were found in the culture filtrates of some genera which limit their own growth (autoinhibitors) while others in the family produce substances which check the growth of other genera (heteroinhibitors). These inhibitors were destroyed by autoclaving. It was decided that Pandorina morum, which produced the strongest inhibitor and Volvox tertius, the most sensitive to the inhibitor would make an excellent model system for a study of the chemical and physical properties of these naturally occurring algicides. The algicide could be removed from actively growing cultures about …

Factors Regulating The Growth Of Algae In Continuous Culture In Diluted Secondary Sewage Treatment Plant Effluent And Subsequent Biodegradability, Edward G. Foree, Caroline P. Wade

KWRRI Research Reports

Heterogeneous algal cultures were grown in laboratory continuous culture in continuous flow, completely mixed chemostats in secondary sewage treatment plant effluent diluted to give an ammonia nitrogen concentration of 10 mg/1. Variables were lighting, pH, carbon dioxide availability, and hydraulic residence time.

Optimum growth occurred under pH 7.0, excess CO₂, and continuous lighting conditions. The availability of artificially supplied excess CO₂ greatly increased the mass (standing crop) at steady-state over that produced under otherwise identical conditions for all residence times studied. For the case of excess CO₂ availability, the nitrogen concentration in the algal cells regulated …

Algal Growth And Decomposition: Effects On Water Quality, Edward G. Foree, John S. Tapp Jr.

KWRRI Research Reports

The chemical composition of algae grown in batch culture depends mainly on environmental conditions, nutrient availability, presence of predators, cell age, and species. The effects of nutrient availability and cell age on the composition of three unialgal cultures (algae + bacteria) and one hetergeneous culture (algae + bacteria + microscopic animals) were evaluated. The cultures were grown in batch culture under both nutrient-abundant and nutrient deficient conditions and the changes in compositions were observed. Luxurious uptake where nutrients are incorporated into cellular protoplasm at levels greater than those necessary for growth, and super-luxurious uptake, where some nutrients are stored rather …