Life Sciences Commons^™

Defining The Genetic Regulation Of Appressorium Formation In Cercospora Zeae-Maydis, Hazel Apiyo Buyu

Graduate Theses and Dissertations

Cercospora zeae-maydis is one of the primary pathogens associated with gray leaf spot, one of the most damaging foliar diseases of maize in the world. Gray leaf spot can be managed to some extent by cultural practices and fungicide applications. To infect maize, C. zeae-maydis grows towards stomata and forms infectious structures, termed appressoria, over stomatal pores. Prior research on the pathogen revealed that appressorium formation is crucial for foliar infection. Although several genes involved in pathogenesis have been identified in C. zeae-maydis, the molecular regulation of appressorium formation in this pathogen is poorly understood. Specifically, how the fungus senses …

Molecular Basis Of Pathogenesis And Host Determination In Cercospora Sojina: From Phenotypic To Genotypic Patterns, Wagner Calegari Fagundes

Graduate Theses and Dissertations

Frogeye leaf spot (FLS), caused by Cercospora sojina, is an important and recurrent disease of soybean in many production regions. Genetic resistance is potentially one of the most cost-effective and sustainable strategies to control FLS. However, C. sojina has already demonstrated the ability to overcome resistance conveyed by single R-genes (resistance genes) of soybeans, followed by the emergence of new physiological races. Although understanding population genomics and the virulence gene inventories in fungal plant pathogens is extremely important to improve disease control measures, studies regarding host specificity and pathogenesis in C. sojina are very limited. Therefore, the overarching goal of …

Understanding And Predicting Nematode Damage On Soybean Using Spatially Weighted Analysis, Barry Boney

Graduate Theses and Dissertations

Aerial imagery offers great potential as a predictive scouting method and could allow growers to better understand crop performance over time. Evidence suggests that the seed treatments fluopyram and abamectin result in decreased reproduction and root galling by Meloidogyne incognita, but yield protection in fields with higher or different nematode pressure is unclear. The objective of this work was to determine the efficacy of these seed treatments compared to 1,3-dichloropropene (1,3-D) applied site-specifically and then predict where these might best be applied to other fields. In a soybean field infested with M. incognita, apparent electrical conductivity was highly correlated with …

Water Conservation Practices For Irrigation Of Turfgrass Lawns, Daniel Sandor

Graduate Theses and Dissertations

Turfgrass lawns are widely used in residential and commercial landscapes, providing many environmental, aesthetic, and recreational benefits. However, concerns and scrutiny arise with perceived maintenance requirements, particularly with regards to irrigation. It is important to manage irrigation in order to maximize water conservation without significantly reducing lawn quality. A series of field and greenhouse studies were conducted to investigate how water usage might be reduced in irrigation practices with the following proposed objectives: 1) evaluate the use of rain and soil moisture sensors in a humid transition zone environment; 2) evaluate the effects of chronic drought stress between drought-resistant and …

Weed Control And Management For Vegetable Soybeans In Arkansas, Seth Bernard Abugho

Graduate Theses and Dissertations

Vegetable soybean [Glycine max (L.) Merr.], known as edamame, needs weed management tools. Releasing locally adapted edamame soybean varieties and registering herbicides are necessary for successful production and expanding the edamame industry. This research aimed to 1) identify herbicides labeled for grain soybean for potential use on edamame; 2) evaluate differential tolerance of edamame soybean varieties to selected grain soybean herbicides; and 3) identify a feasible edamame-based crop rotation system. For objective 1, 26 herbicide treatments were tested on AVS-4002 edamame including preplant (PPL), preemergence (PRE) and postemergence (POST) herbicides labeled for grain soybean. Preplant herbicides caused 9 to 28% …

Evaluation And Characterization Of Fitness Costs In Multiple Herbicide Resistant Echinochloa In Arkansas, Teal Marie Penka

Graduate Theses and Dissertations

Echinochloa spp. are major weed problems for rice (Oryza sativa L.) on a global scale. In the southern rice belt of the U.S.A., Echinochloa colona (L.) Link (junglerice) and E. crus-galli (L.) P. Beauv (barnyardgrass) are the most troublesome weeds. These species are morphologically diverse, and its high ploidy level augurs high genetic diversity, which favors high adaptability to stress, including herbicide selection pressure. The objectives of these studies were to 1) isolate resistant (R) and sensitive (S) biotypes from eight accessions of E. colona with different resistance profiles; 2) determine the resistance levels of selected R and S biotypes …

Influence Of Loss Of Function Of The Fatty Acid Desaturase 7 Gene On Photosynthetic Activity And Foliar Redox Status, Janithri Savindya Wickramanayake

Graduate Theses and Dissertations

Fatty Acid Desaturase7 (FAD7) is a chloroplast-localized enzyme that converts 16 and 18 carbon dienoic fatty acids to trienoic fatty acids. The suppressor of prosystemin-mediated response2 (spr2) mutant in tomato (Solanum lycopersicum) and the fad7-1 mutant in Arabidopsis (Arabidopsis thaliana) result in the loss of function of FAD7, which alter the fatty acid profiles of chloroplast membranes and enhance resistance against aphids. This research contributes toward the long-term goal of identifying factors that determine aphid resistance in FAD7 mutants. Previous data suggested constitutive differences between spr2 and WT including increased expression of genes associated with photosynthesis and differences in redox …

Linkage Mapping For Soybean (Glycine Max) Flood Tolerance, Wade Stiles Hummer

Graduate Theses and Dissertations

Flood tolerance in soybean (Glycine max) is not a well-characterized trait, yet flooding damage is second only to drought stress in terms of yield reduction. The objectives of this study were to determine genetic variation for flooding tolerance in two populations of soybean recombinant inbred lines (RILs) and to identify and confirm flood tolerant QTL. Population A (WHA) consisted of 111 RILs derived from the cross 5002T by 91210-350 and Population B (WHB) consisted of 79 RILs from the cross RA-452 by Osage. Experiments were conducted at the Rice Research and Extension Center (RREC) near Stuttgart, AR in 2015 and …

Evaluation Of Rice Stink Bug, Oebalus Pugnax (F.), Damage And Monitoring Techniques In Rice, Oryza Sativa L., And Grain Sorghum, Sorghum Bicolor (L.), Aaron Joseph Cato

Graduate Theses and Dissertations

Rice stink bug, Oebalus pugnax (F.), is a serious pest of headed rice, Oryza sativa L. and an occasional pest of heading grain sorghum in the Mid-south. Work from this dissertation focuses on resolving gaps in and knowledge of rice stink bug sampling and management, and attempts to create a basis for rice stink bug damage assessment in future studies.

Field experiments were conducted from 2016-2018 to asses variation in sweep net sampling by observing producers, researchers, extension personnel, consultants and their workers. Large levels of variation were found in sweep lengths between observed sweepers and reliability of smaller sweep …

Evaluation Of Soybean Maturity Group And Planting Date In A Soybean-Rice Rotation On Overall Crop Productivity, Carrie Ortel

Graduate Theses and Dissertations

Little is known about the effects of soybean (Glycine max L.) management techniques on soil-nitrogen (N) credit development and its impact on the subsequent rice (Oryza sativa L.) crop’s success. This study was conducted to determine how soybean maturity group (MG) and planting date effect overall soybean productivity and its influence on the following rice crop. Various soybean planting dates (optimum and late) and MGs (3.5, 4.7, 5.4, and 5.6) were grown and followed in rotation with a rice crop. Six rates of pre-flood fertilizer-N (0, 44, 89, 134, 179, 224 kg N ha-1) were applied to the rice crop. …

Physiological Characterization Of The Soynam Parental Lines Under Field Conditions, Akshita Mishra

Graduate Theses and Dissertations

The narrow genetic pool of soybean (Glycine max L. Merr.) in North America can limit its future yield gains. Among the worldwide germplasm collection of 45,000 unique landraces, only 80 contribute 99% to the collective parentage of North American soybean cultivars. Among these 80 landraces, just 17 contribute to 86% of the collective parentage of the modern cultivars. The Soybean Nested Association Mapping population (SoyNAM) was therefore developed with the objective of diversifying the soybean gene pool. Forty diverse soybean genotypes from maturity groups (MG) 1 through 5 were crossed with a common MG 3 parent to develop 40 recombinant …

Systems To Attract And Feed Pollinators In Warm-Season Lawns, Michelle Wisdom

Graduate Theses and Dissertations

Pollinating insects are responsible for the production of many agricultural crops and they require floral resources to fulfill their life-cycle. Ideally, pollinating insects will encounter a diversity of floral resources across their entire season of activity, and those floral resources can include both herbaceous and woody plant species. Managed turfgrass areas have been identified as potential locations for creating pollinator-friendly habitats. In the transition zone, where both warm- and cool-season turfgrass species are present, the persistence of herbaceous plants in warm-season turfgrasses such as bermudagrass (Cynodon spp.) and buffalograss (Buchloe dactyloides), may be affected not only by the competitive nature …

Mitigating The Risk For Glufosinate Resistance, Christopher John Meyer

Graduate Theses and Dissertations

While glufosinate-resistant weeds have not yet been identified in U.S. row crops, rapid evolution of glyphosate-resistance, and resulting cost to U.S. farmers, demonstrates the need to responsibly steward the limited number of herbicides available in agricultural systems. Field and laboratory experiments were conducted to: 1) Identify herbicide interactions that can occur in Enlist™ and Roundup Ready® Xtend® systems; 2) Identify herbicide interactions that occur when glufosinate is mixed with fomesafen, clethodim, and glyphosate; 3) Determine the optimum rate structure and application timings when multiple POST applications of glufosinate are applied to LibertyLink soybean; 4) Use 14C techniques to determine why …

Evaluation Of Acetochlor And Other Very-Long-Chain Fatty Acid-Inhibiting Herbicides In Arkansas Rice, Michael E. Fogleman

Graduate Theses and Dissertations

The prevalence of herbicide resistance and lack of effective management options for controlling problematic weeds such as barnyardgrass and weedy rice in Arkansas rice production has led to exploration of alternative herbicide sites of action (SOA). Very-long-chain fatty acid (VLCFA)-inhibiting herbicides are not currently labeled for use in U.S. rice production but have been used with success in other row crops and in rice production in Asia. Based on preliminary research, rice tolerance and weed control were evaluated following various application timings and rates of acetochlor and pethoxamid, in addition to several other VLCFA-inhibiting herbicides. Rice tolerance to acetochlor was …

Effect Of Allelic Variation In Rht Loci On Plant Height And Grain Yield Of Soft Red Winter Wheat (Triticum Aestivum L.), Habib Hayat

Graduate Theses and Dissertations

Plant height in wheat (Triticum aestivum L.) is controlled in large part by two major Rht genes, Rht-B1 and Rht-D1, which pleiotropically impact lodging and grain yield. Prior to the Green Revolution, wheat varieties contained only ‘wild-type’ Rht alleles (Rht-B1a and Rht-D1a) and were tall and prone to lodging. Introgression of a semi-dominant mutation at either of these two loci (Rht-B1b or Rht-D1b) results in a semi-dwarf phenotype and reduced plant height. When combined (Rht-B1b and Rht-D1b) an extremely short double-dwarf phenotype is observed. The objective of this study was to determine the impact of allelic variation in Rht-B1 and …

Dissection Of Soil Waterlogging Tolerance In Soft Red Winter Wheat Using Genomic Approaches, Marlovi Andrea Acuna-Galindo

Graduate Theses and Dissertations

Genomic methods including genome wide association analysis (GWAS), genomic selection (GS) and RNA-seq allow for faster selection of superior breeding lines and for identification and resolution of candidate genes. A panel of 240 soft red winter wheat (Triticum aestivum L.) cultivars and breeding lines were subjected to soil waterlogging stress over two seasons at Stuttgart, AR and St. Joseph, LA, US. Total concentrations of P, K, Ca, Mg, Mn, Fe, Al, B, Cu, Na, S and Zn were determined in wheat shoots post-waterlogging using inductively coupled plasma spectroscopy. Yield components kernel number per spike (KNPS), kernel weight per spike (KWS) …

Association Mapping And Genomic Selection For Yield And Agronomic Traits In Soft Winter Wheat, Dennis Bulusan Lozada

Graduate Theses and Dissertations

Tools such as genome-wide association study (GWAS) and genomic selection (GS) have expedited the development of crops with improved genetic potential. While GWAS aims to identify significant markers associated with a trait of interest, the goal of GS is to utilize all marker effects to predict the performance of new breeding lines prior to testing. A GWAS for grain yield (GY), yield components, and agronomic traits was conducted using a diverse panel of 239 soft winter wheat (SWW) lines evaluated in eight site-years in Arkansas and Oklahoma. Broad sense heritability of GY (H2=0.48) was moderate compared to other traits including …

Methane Emissions From Rice Production On A Silt-Loam Soil In Arkansas, Joshua Jeffry Humphreys

Graduate Theses and Dissertations

Methane (CH4) emissions from rice (Oryza sativa L.) production are a source of concern in the environmental and agricultural communities. New and/or revised agronomic methodologies will be needed to identify production practice combinations that reduce CH4 emissions without decreasing yields. The objective of this multi-year study was to evaluate the effects of water management (i.e., full-season flood and mid-season drain) (2015), cultivar (i.e., pure-line cultivar ‘LaKast’ and the RiceTec hybrid “XP753”) (2015), soil organic matter (SOM) concentration (2016), and tillage [conventional tillage (CT) and no-tillage (NT)] and urea-based fertilizers [N-(n-butyl) thiophosphoric triamide (NBPT)-coated urea and non-coated urea] (2017) on CH4 …

Species And Cultural Management Of Earthworms On Golf Course Turf In Arkansas And Oklahoma, Paige Elizabeth Boyle

Graduate Theses and Dissertations

As earthworms feed, they can egest soil and nutrient-rich aggregates (casts) on the soil surface. In low-cut turfgrass systems, such as golf course greens, tees, and fairways, surface casting can result in a muddy playing surface, ball roll issues, weed and pest invasion, reduced aesthetics, surface softening, and reduced photosynthesis. Because the use of pesticides for earthworm control is illegal in the U.S., earthworm casting must be managed through cultural practices. Sand topdressing is one method of earthworm control studied for use on golf courses, with the supposition being that the abrasive sand particles will deter the soft-bodied earthworms from …

Determining The Light Requirements Of Various Turfgrass Systems And Investigating Techniques To Quantify Photosynthetically Active Radiation, Travis Russell

Graduate Theses and Dissertations

Light is one of the primary growth-limiting factors of turfgrass. Without sufficient quantities of photosynthetically active radiation (PAR; 400-700 nm wavelength), turfgrass quality will decline and could ultimately result in plant death. PAR light can be expressed in a daily quantity known as the daily light integral (DLI) and has been utilized to determine minimum light requirements for turfgrass as well as other plants. With an estimated 25% of all turfgrass being maintained under some level of shaded conditions, it is imperative to define minimum DLI requirements of various turfgrass systems to ensure acceptable turf quality. In addition, it is …

Genetic And Phytopathological Studies On Charcoal Rot Resistance In Soybean [Glycine Max (L) Merr.], Marcos Paulo Da Silva

Graduate Theses and Dissertations

Charcoal rot of soybean (Glycine max (L.), caused by Macrophomina phaseolina is a disease of economic significance in the United States. The identification and quantification of the resistance is difficult, and very little is known about the genetics and markers linked to the charcoal rot (CR) resistance genes. Current assay methods can be time consuming, and data may vary between tests. The objectives of this study were to 1) create a robust seed plate assay (SPA) for CR resistance by comparing results with cut-stem and CFUI assays; 2) correlate and compare field data disease assessments with SPA; 3) identify QTLs …

Nitrous Oxide Emissions From Rice Production On A Silt-Loam Soil In Arkansas, Casey Rector

Graduate Theses and Dissertations

Rice (Oryza sativa L.) is a common crop grown in Arkansas under flooded-soil conditions. The saturated to nearly saturated soil makes rice production an ideal environment for the production of potent greenhouse gases, such as nitrous oxide (N2O). The objectives of this study were to i) evaluate the impact of water management practice (full-season-flood and intermittent-flood) and cultivar (pure-line and hybrid) on N2O fluxes, season-long N2O emissions, and global warming potential (GWP; 2016) and ii) evaluate the impact of tillage practice [conventional tillage and no-tillage (NT)] and type of urea fertilizer [ N-(n-butyl) thiosphosphoric triamide (NBPT)-coated and non-coated urea] on …

Sustainable Intensification Of Agriculture: Opportunities And Challenges For Food Security And Agrarian Adaptation To Environmental Change In Bangladesh, Aaron Michael Shew

Graduate Theses and Dissertations

This dissertation investigates three unique aspects of sustainable agricultural intensification (SAI) in the context of Bangladeshi rice production. The first article presents a qualitative analysis of SAI and farmer surveys in the embanked polder region of coastal Bangladesh. The second article investigates the global food security and environmental impacts of already adopted High Yielding Variety (HYV) rice and double-cropped rice systems in Bangladesh using a spatial partial equilibrium trade model and a Life Cycle Assessment (LCA). The final article demonstrates a remote sensing methodology for monitoring dry season rice production at 30 m resolution in Bangladesh using a harmonic time …

An Evaluation Of Breeding, Agronomic, And Processing Methodologies Of Vegetable Soybean (Edamame) To Increase Domestic Production In The United States Market, David Octor Moseley

Graduate Theses and Dissertations

Edamame is a food-grade soybean (Glycine max (L.) Merrill) that is either harvested immature (R6 stage) or after plant maturity (R8 stage). At R6, the premium product will have crescent- shaped large green pods and gray pubescence. At R8, the seed will either have stayed green or will have turned yellow, black, or brown. Edamame is a healthy snack with a sweet flavor and firm texture. The edamame market is growing in the United States, creating a need for more adapted varieties. The genetic diversity is low among adapted large-seeded breeding lines. Finding diverse accessions will help develop larger and …

Soil Properties That Influence The Occurrence Of Hydrogen Sulfide Toxicity In Rice Fields, Julia Marie Fryer

Graduate Theses and Dissertations

Rice (Oryza sativa L.) producers face many challenges throughout each growing season. Hydrogen sulfide (H2S) toxicity is a physiological disorder where sulfate (SO42-) is excessively reduced to the toxic gas, H2S. This can reduce yield and, in severe cases, result in crop death. The main research objectives were to: i) understand chemical and physical characteristics in soils prone to H2S toxicity, ii) determine influential soil characteristics on the incidence of H2S toxicity, iii) determine ammonium sulfate ((NH4)2SO4) fertilizer additions influence on H2S toxicity, and iv) predict when and where H2S will occur. Three greenhouse experiments were conducted using Arkansas field …

Consumers’ Willingness To Pay For Hydroponic Lettuce, Daniel Gilmour

Graduate Theses and Dissertations

With continued advances in hydroponic plant production technology, an increasing number of farms have begun using hydroponic techniques to grow leaf lettuce and other food crops in a controlled environment. Recent controversy about the ongoing inclusion of hydroponics in the USDA organic program has highlighted uncertainty about marketing for hydroponic crops. In November 2017, the National Organic Standards Board voted not to recommend that hydroponic farms be banned from applying for organic certification. Since then, continued controversy has led a group of organic producers to start an additional independent certification program that would exclude hydroponic crops. While hydroponic production may …

Irrigation Practice Adoption: Causes And Consequences In The Arkansas Delta, Kerr James Adams

Graduate Theses and Dissertations

Concerns about groundwater depletion from conventional irrigation agriculture in the Arkansas Delta region have led to the promotion of more efficient irrigation practices. With Arkansas being the largest producer of rice, the 10th largest producer of soybeans and the 16th largest producer of corn in the United States, the irrigation demand of these crops has put pressure on producers to find ways to irrigate more efficiently. Not only are the alternative technologies supposed to reduce water use, it is also believed that their adoption can also yield economic benefits for the producer. Despite these assumed benefits, adoption of alternative technologies …

A Genome Wide Association Study For Fusarium Head Blight Resistance In Southern Soft Red Winter Wheat, Amanda Holder

Graduate Theses and Dissertations

Fusarium head blight (FHB) is a disease of small grains caused by the fungal pathogen Fusarium graminearum. FHB poses potential economic losses and health risks due to the accumulation of the mycotoxin deoxynivalenol (DON) on infected seed heads. The objectives of this study are: 1) evaluate soft red winter wheat (SRWW) lines for resistance to FHB in terms of resistance to initial inoculum (incidence); resistance to spread within the head (severity); resistance to DON accumulation; and resistance to Fusarium damaged kernels (FDK), 2) determine the frequency and effect of known FHB resistance genes and quantitative trait loci (QTL), and 3) …

Dicamba Effects On Soybean (Glycine Max) Growth, Yield, And Offspring, Mark Spencer Mccown

Graduate Theses and Dissertations

Introduction of a new dicamba-resistant (Xtend) trait in soybean and cotton will increase dicamba herbicide use. Consequently, concern for injury to sensitive crops from off-target movement and tank contamination will likely increase. For soybean, foliar symptoms associated with dicamba damage do not necessarily reflect yield losses; hence, experiments were conducted to determine the effects of dicamba on soybean growth, yield, and offspring. Low rates of dicamba [1/64X (8.75 g ae ha-1) and 1/256X (2.18 g ae ha-1) of a normal 1X field rate (560 g ae ha-1)] were applied at two vegetative growth stages (V4, V6) and at each reproductive …

Evaluation Of Dicamba Off-Target Movement And Subsequent Effects On Soybean Offspring, Gordon Travis Jones

Graduate Theses and Dissertations

Commercial launch of cotton with resistance to dicamba, glyphosate, and glufosinate occurred in 2015 and launch of soybean with resistance to dicamba and glyphosate occurred in early 2016. It is likely that non-dicamba-resistant soybean will be planted in close proximity to dicamba-resistant soybean and cotton. Therefore, experiments were conducted to examine the distance dicamba moves during an application using commercial application equipment, as well as the effect the drift events have upon soybean offspring. Additional experiments were designed to investigate the effect glyphosate addition to dicamba has upon soybean growth and yield as well as possible effects on offspring. Lastly, …