Life Sciences Commons^™

Agronomic And Economic Comparison Of Potential Winter Oilseed Species And Varieties For Tennessee, Stefanie B. Keadle

Masters Theses

Winter oilseed crops are annual cool season Brassica crops which fit well within typical corn and soybean rotations. They are currently being adopted in Midwestern conventional agriculture systems. The ability to produce sustainable aviation fuel (SAF) from oil pressed from the seeds is the driving force of recent interest in the crops. The three main crops of interest in this study are canola (Brassica napus), pennycress (Thlaspi arvense L.), and camelina (Camelina sativa). To determine if production of winter oilseeds is feasible in Tennessee, three experiments were conducted. First, a field study variety trial was …

Improving Water And Plant Growth Regulator (Pgr) Management Practices In Cotton: Cultivar Physiological Responses And Pgr Rates, Kurt Wedegaertner

Masters Theses

Cotton (Gossypium hirsutum L.) is grown in an increasingly unpredictable climate and often under rainfed conditions. Without irrigation to alleviate common drought conditions, cultivar selection becomes a primary tool in mitigating the negative effects of water-deficit stress. Cultivars can differ in water use under water-limited environments, which can affect crop performance. Moreover, cotton management must involve balancing vegetative and reproductive growth through the use of plant growth regulators (PGR) due to the plant’s perennial growth pattern. The efficacy of PGRs are dependent on many variables including environmental conditions, irrigation, and genetics (cultivar). In 2020 and 2021, a series of …

Identifying Drought Tolerance Traits In Tennessee Soybean Genotypes: Recovery From Severe Water Deficit Stress, Samuel W. Purdom

Masters Theses

Ninety-four percent of U.S. grown soybeans are produced under rainfed conditions with intermittent droughts occurring late in the season during reproductive growth stages. Due to the temporary nature of drought, the ability of a crop to survive and recover effectively from water deficit stress is important for ensuring yield stability. In 2019 and 2020, two greenhouse experiments and two field studies were conducted to screen eleven soybean genotypes for transpiration response and recovery from water deficit stress and high vapor pressure deficit (VPD). In the first greenhouse study, soybean plants were grown in pots sealed to prevent evaporation and plants …

Determining Critical Fall Height For Bermudagrass Grown On Sand And Native Soil Root Zones, Taylor L. Williams

Masters Theses

Surface hardness is typically measured using a Clegg Impact Soil Tester (ASTM F355-D), but recent trends in sports turf are to use the F355-E missile as a potential alternative, because it may provide a more meaningful measurement. Forty traffic events were applied to ‘Tifway’ bermudagrass grown in an ASTM constructed sand root zone and a silt loam soil on the Center for Athletic Field Safety at the University of Tennessee. An F355-E was used to measure head injury criteria (HIC) for both root zones after every eight traffic events. Head injury criteria values were regressed to varying drop heights to …

Forage Species Selection For Transitional Organic Production In The Southeastern United States, Jonathan Kubesch

Masters Theses

Despite the vast production markets for forage and organic products nationally, so far limited work has been done to develop organic forages specifically for Middle Tennessee or the mid-South in general. The present organic research field focuses on vegetable and grain production; however, forage production offers an easier transition for producers moving into certified organic agriculture. The present study seeks to evaluate several forage blends for optimizing forage production under low-input transitional organic conditions. Ideally a forage system could be tailored to the beef cattle operations of Middle Tennessee, the dominant forage consumption market in this region of the mid-South. …

Switchgrass Extractives Have Potential As A Value-Added Antimicrobial Against Plant Pathogens And Foodborne Pathogens, Alexander Ian Bruce

Masters Theses

Panicum virgatum (switchgrass), a perennial grass native to North America, is a leading biomass feedstock candidate for the manufacture of cellulosic ethanol. Switchgrass is considered a viable option for biofuel production due to its cheap production cost and ability to grow on marginal land. Biofuel derived from switchgrass has been shown to be very energy efficient, producing 540% more renewable energy versus nonrenewable energy expended. Switchgrass-derived biofuel is also estimated to have greenhouse gas emissions that are 94% lower than emissions from gasoline (Schmer et al 2008). Biofuels are created through biochemical processes that utilize various enzymes and microorganisms for …

Influence Of Application Technology On Foliar Fungicide Efficacy On Cercospora Sojina Infected Soybean, Shawn Alan Butler

Masters Theses

Due to the constant concern with off-target contamination and application technology requirements associated with future herbicide-tolerant crops, the use of drift-reduction nozzle technology (DRT) may increase. The primary objective of this research was to evaluate the effects of coarse droplets generated by drift-reduction nozzles on foliar fungicide efficacy and residual in soybean infected by frogeye leaf spot caused by Cercospora sojina. No differences in disease control, soybean yield, spray retention, and residual when applying Quadris Top SB, a premix of azoxystrobin and difenoconazole, using nozzles that produce either a medium or ultra coarse droplet spectrum were determined.

Due to …

Evaluation Of Fungicide Efficacy Against Cercospora Sojina And Selection For Qoi-Fungicide Resistance In Soybean, Alicia Mercedes Cochran

Masters Theses

Frogeye leaf spot (FLS) of soybean is caused by Cercospora sojina. In 2010, resistance to the quinone outside inhibitor (QoI) fungicides was reported. Since then, evaluating FLS for QoI-resistance has been of particular interest in Tennessee and other soybean-producing states. In order to determine alternative fungicide options, fungicides with solo and combination mode of action were tested in 2013-2014. The objectives were,1) to evaluate fungicide efficacy for disease control and yield protection, and 2) evaluate selection pressure for QoI-resistance. Treatments included the following fungicide groups: QoI, DMI, MBC, Chlorothalonil, QoI+DMI, SDHI+QoI, SDHI+QoI+DMI, and DMI+MBC. QoI and Chlorothalonil treatments failed …

Impacts Of Biofumigation And Anaerobic Soil Disinfestation On Strawberry Production, Jennifer Renee’ Wheeler

Masters Theses

Due to the phase-out of methyl bromide, there is a need for alternative, nonchemical fumigation treatments in strawberry production. Anaerobic soil disinfestation and biofumigation are two non-chemical methods for controlling soilborne plant pathogens of strawberry. This study was designed to observe strawberry fruit nutrition and soil volatiles of a strawberry field being treated with biofumigation treatments, anaerobic soil disinfestation treatments, and a combination of the two alternative methods. A trial was conducted with 11 pre-plant soil-incorporated treatments arranged in a randomized complete block design with 6 rows (blocks). Biofumigation treatments consisted of deactivated mustard meal, deoiled mustard meal, mustard pellets, …

Improving Management And Species Selection Of Warm-Season Forage Grasses For Southeast Production Systems, Christine Helen Gelley

Masters Theses

Beef producers need drought tolerant options when selecting forage grasses and also practical methods to estimate forage nutritive value, which this study aims to provide for warm-season grasses. The objective of the first experiment was to develop estimates of warm-season forage nutritive value and herbage mass based on harvest timing. The experiment was conducted from 2013 to 2015 at the University of Tennessee Plateau AgResearch and Education Center (PREC) in Crossville, TN. Four species were evaluated, each for two years: bermudagrass [Cynodon dactylon (L.) Pers. cv. Vaughn’s # 1], switchgrass [Panicum virgatum (L.) cv. Alamo], sorghum-sudangrass [Sorghum …

Cowpea Adaptability To Southeastern Organic Farming Systems: Forage Productivity And Charcoal Rot Susceptibility, Samantha Lindsey Hill

Masters Theses

Cowpea (Vigna unguiculata (L.) Walp.) is a warm-season, multi-purpose legume that is well-adapted to the southeastern USA and has many traits that make it an attractive forage or cover crop for integration into organic production systems, including high rates of nitrogen (N) fixation, phosphorus (P) use efficiency, regrowth ability, and high digestibility. Eight cowpea varieties were evaluated under organic management at two locations in summer 2014 for stand establishment, forage yield and quality, and weed biomass.

Charcoal rot [Macrophomina phaseolina (Tassi) Goid.] is a fungal disease that is economically important to many host plant species. High temperatures and …

Physiological Evaluation And Root Morphological Differences Associated With Novel Drought Tolerant Corn (Zea Mays L.) Hybrids, Austin Gentry Scott

Masters Theses

Corn hybrids designated “drought tolerant” (DT) from three commercial seed companies were evaluated in order to determine the benefit of DT technology in a mid-south environment that may receive adequate but often poorly timed rainfall. Field research was conducted in 2014 and 2015 at Milan, TN where hybrid response to stress was evaluated at two planting dates and in non-irrigated and irrigated environments and greenhouse research was conducted in Jackson, TN to evaluate early season drought to plant physiology and root structure.

In 2014 and 2015, the non-irrigated trials received enough rainfall to create a high yielding environment. Since the …

Molecular Marker Assisted Backcross Development And Evaluation Of An Environmentally Friendly, Commercially Acceptable Low Seed Phytate Soybean, Jeffrey David Boehm Jr.

Masters Theses

Soybean [Glycine max (L). Merrill] is the world’s top oilseed crop. High protein soymeal is a primary soybean use and staple food of livestock and fish. The storage form of phosphorus (P) in the seed is phytic acid, or phytate [myo-inositol 1,2,3,4,5,6 hexakisphosphate] which binds and chelates key nutritional cations forming stable salts that are non-digestible. Livestock waste laden with phytate P is an environmental concern, and a source of nonpoint P pollution causing eutrophication and surface water quality deterioration. The primary objective of this research was the development of a commercially acceptable low phytate (LP) soybean …

Effects Of Organically Enhanced Biofertilizer And Fertilizer Briquettes On Mineral Nutrition, Quality, And Yield Of Corn And Soil Health, John Heggie Winings

Masters Theses

Interest in alternate fertilizers has increased in recent years in order to improve crop nutrition and soil health. The efficacy of these fertilizers on corn (Zea mays L.) production and quality has been scarcely documented. Two alternative fertilizers, organically enhanced NPSFe Unity biofertilizer (Unity) manufactured from sterilized organic additives extracted from municipal wastewater biosolids and NPKZn briquettes (briquettes) produced by compacting solid fertilizers into a super-granule between 1-3 grams, were evaluated for nutrient concentrations, growth, quality, and yield of corn, residual soil nutrient levels, and soil microbial community structure relative to the conventional fertilizers ammonium sulfate and urea at …

Synthetic Turf Surface Temperature Reduction And Performance Characteristics As Affected By Calcined Clay Modified Infill, Eric Hall Reasor

Masters Theses

Synthetic turf research plots containing crumb rubber (CR) infill were established in Knoxville, TN in 2012 and 2013. Calcined clay (CC) was amended to CR in several ratios: a 50:50 (vol vol-1) blend; a 50:50 blend with a polymer coating on CC (50 CR:50 CCC); a 15 mm layer of CC under 15 mm of CR (CR over CC); and a 15 mm layer of CC over 15 mm of CR (CC over CR). A 100% CR and a 70:30 blend of CR to sand (70 CR:30 S) were included for comparison. Surface temperature was measured in the summer of …

Forage And Biomass Dual-Purpose Harvest System Using Native Warm-Season Grasses, David Weston Mcintosh

Masters Theses

There has been increasing interest in utilizing native warm-season grasses (NWSGs), especially switchgrass, as a biomass feedstock for cellulosic ethanol production. Millions of hectares of crop and pasture in the mid-South are forecast to potentially be planted with switchgrass for biomass feedstock production. This could have a substantial impact on the region’s cattle industry, reducing forage production hectares. This study was conducted to determine the effect of early season harvest timing on forage and biomass of NWSGs designed for use in cellulosic ethanol production. The over-all hypothesis was to determine if an early forage harvest can be included in a …

Preservation Of Nutrients In Cool- And Warm-Season Forages At Different Stages Of Maturity And Management, Jason Allen Shultz

Masters Theses

The overall goals of the studies described in this thesis were to investigate management practices of tall fescue and native warm-season grasses (NWSG) and find the best time to harvest and method to preserve forage quality. Study one investigated the effects of maturity on tall fescue and switchgrass and the effects of preservation method on forage quality. This study confirmed that maturity reduced forage quality in both tall fescue and switchgrass. Both tall fescue and switchgrass were successfully preserved as haylage or hay and did not differ in forage quality. Forages harvested before mid-May met the TDN and CP requirements …

Comparison Of Roundup Ready And Conventional Soybean (Glycine Max L.) Weed Control Systems For Optimizing Yield And Economic Profitability, Brittany Lee Gaban

Masters Theses

Research was conducted in 2010, 2011 and 2012 at the East Tennessee Research and Education Center in Knoxville, TN, in order to compare differences in soybean yield among differing levels of weed control within Roundup Ready® [Glyphosate-resistant] (RR)and conventional soybean cultivars to gain a better understanding of the impact different intensities of weed control have on RR and conventional cropping systems. Results determined that after applying the weed control regimens, there was no significant difference (p

Glyphosate resistant weeds introduce new challenges and create a more costly weed control regimen, especially when using a RR based soybean cultivation operation. Therefore, …

The Effect Of Photocatalytic Titanium Dioxide And Ultraviolet B Radiation On Sclerotinia Homoeocarpa Growth And Pathogenicity, Chun Hu

Masters Theses

Photocatalytic TiO₂ [titanium dioxide] generates strong oxidative effects when illuminated with ultraviolet (UV) light with wavelengths of less than 385 nm. UVB has wavelengths ranging from 280 to 315nm. Previous research indicates the oxidative species and UVB radiation can react and cause cellular damage to microorganisms, which may reduce Sclerotinia homoeocarpa growth and help to control dollar spot disease development. The objectives of this study were to investigate the interactions of TiO₂ and UVB radiation, both in vitro and in vivo, on the growth and development of dollar spot. Factorial treatments consisting of five rates of TiO₂ and three doses …

Anaerobic Soil Disinfestation: Evaluation Of Anaerobic Soil Disinfestation (Asd) For Warm-Season Vegetable Production In Tennessee, David Grant Mccarty

Masters Theses

Anaerobic soil disinfestation (ASD) is a non-chemical, pre-plant soil treatment recently developed for control of pests such as soilborne plant pathogens, plant-parasitic nematodes, and weeds in specialty crop systems. Soil treatment by ASD includes incorporating a labile carbon (C) source, tarping with plastic, and irrigation of the topsoil to saturation to facilitate the development of strongly anaerobic soil conditions driven by soil microbes. Processes occurring during the anaerobic decomposition of the added C source have been reported control plant pests. The goal of this project was to evaluate and adapt the ASD procedure to environmental conditions and production systems in …

Applicability Of Pigment Compounds For Reducing Light Stress In Bentgrass, Gregory Keith Bartley

Masters Theses

Chlorinated copper phthalocyanine (Signature) and pulverized cells of Chlorella vulgaris (Chlorella) were evaluated in a controlled environment for their ability to act as photoprotectants under supraoptimal levels of ultraviolet (UV) and photosynthetically active radiation (PAR) when applied to plant leaves. Plant pigment changes were documented using High Performance Liquid Chromatography following 1 week of exposure to supraoptimal light in two separate experiments incorporating UV (106.6 μmol m-2 s-1) and PAR (760.6 μmol m-2 s-1) over a 12h photoperiod. Supraoptimal levels of UV and PAR light were found to cause significant reductions in Agrostis palustris chlorophyll and carotenoid leaf pigment levels. …

Heritability And Genetic Gain Of Seed Protein, Oil, And Yield Among Ril Of Soybean, Benjamin T. Wiggins

Masters Theses

Soybean is a remarkable crop that is used in many aspects of our everyday lives. The seed protein is used primarily for livestock feed, though it is becoming more available as a meat substitute for human consumption. Soybean oil is used in many products such as plastics, foods, and industrial products. Seed yield is the major trait that is selected for in a breeding program. A population of 239 F₄ derived RIL from the cross of USG 5601T × U99-310255 was used to calculate heritability estimates by parent offspring regression and on an entry mean basis. This population was …

Utility Of Aminocyclopyrachlor For Control Of Horsenettle And Tall Ironweed In Cool-Season Grass Pastures, William Paul Phillips

Masters Theses

Previous research has shown that management of horsenettle (Solanum carolinense) and tall ironweed (Vernonia gigantea) in cool-season grass pastures and hayfields is difficult. Therefore, research was conducted at Alcoa, Fork Creek, Greenback, Maynardville, and Pulaski, Tennessee, and at London, Kentucky, in 2010 and 2011 to examine efficacy of aminocyclopyrachlor on these two perennial weeds. Research was conducted on naturally-occurring infestations of each weed. Treatments were: aminocyclopyrachlor (49 g ai/ha) with and without 2,4-D amine (371 g ai/ha), aminocyclopyrachlor (98 g ai/ha) with and without 2,4-D amine (742 g ai/ha), and aminopyralid (88 g ai/ha). Treatments were …

Cotton (Gossipium Hirsutum L.) Response To Irrigation And Environment In A Short Season Climate, Matthew Scott Wiggins

Masters Theses

Research was conducted in 2010 and 2011 at the West Tennessee Research and Education Center in Jackson, TN to investigate water deficit and irrigation response in cotton to provide a better understanding of physiological growth changes and yield impact on the crop grown in soils of varying depth to a sandy layer. The deep soil yielded more vegetative mass when compared with the shallower soil. This is also true when applying higher rates of irrigation where plants grew two more nodes of growth and 15.2 centimeters of plant height. Time to cotton maturity was delayed seven days in the deep …

Molecular Marker Assisted Development And Stability Analysis Of Low Phytate, High Inorganic Phosphorus Soybean [Glycine Max (L.) Merrill], Suzannah Joy Wiggins

Masters Theses

Phytate [myo-inositol 1,2,3,4,5,6-hexakisphosphate] is a mixed cation salt of phytic acid which binds to other minerals, in many field crops including soybean [Glycine max (L.) Merr.]. Livestock with monogastric digestive systems lack the ability to break bonds between phytate and these minerals, causing phytate phosphorus (P) to be excreted in the waste and contributing to possible P and other mineral deficiencies. Discovery of single sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers associated with low phytate QTL (cqPha-001 and cqPha-002) have aided in development of low phytate soybean lines. The objectives of this study were …

Integrated Strategies For Controlling Warm-Season Turfgrass Weeds, Matthew Thomas Elmore

Masters Theses

Herbicidal inhibitors of 4-hydroxyphenylpyruvate dioxygenase (HPPD) such as mesotrione, topramezone and tembotrione were evaluated in greenhouse experiments for activity against bermudagrass. While topramezone and tembotrione exhibited greater activity than mesotrione, none of these herbicides provided acceptable bermudagrass control. These herbicides reduced leaf tissue chlorophyll and carotenoid pigment concentrations in bermudagrass. Changes in turfgrass pigmentation were quantified using HPLC analogy as well as evaluations of visual bleaching and measurements of chlorophyll fluorescence yield (F_v/F_m). Results indicated that these more expeditious methods of evaluating HPPD-inhibiting herbicide activity (visual evaluations and F_v/F_m) cannot be used …

Impact Of Switchgrass Bioenergy Feedstock Production On Soil Carbon Dioxide Flux And Below Ground Soil Organic Carbon Storage In East Tennessee, Leah Denise Soro

Masters Theses

Bioenergy production from switchgrass has shown promise in restoring degraded soils and helping to mitigate greenhouse gas (GHG) emissions. CO₂ loss and C-sequestration in soils are important topics for research to better understand the environmental impacts of bioenergy crops. The need for more thorough research of the carbon cycle in soils used for bioenergy production precipitated the primary interest of this study. The specific objectives of this study were 1) to measure SOC under switchgrass production in order to predict storage of carbon in soils based on previous cropping history, land management, soil physical characteristics, and time; and to …

Establishment And Persistence Of Legumes In Switchgrass Biomass And Forage/Biomass Production Systems, Kara Spivey Warwick

Masters Theses

Switchgrass, Panicum virgatum, is being developed as an economically and ecologically sustainable biomass crop. Nitrogen is considered one of the most limiting inputs of switchgrass. Alternatives to synthetic nitrogen fertilization may be nitrogen-fixing legumes interseeded into switchgrass. The objectives of this research were: (1) develop efficient legume management strategies for switchgrass production systems, (2) evaluate and identify cool and warm-season legumes that can be grown compatibly with switchgrass, (3) determine whether switchgrass yields are increased by legume N-fixation, and (4) determine N-fixation of common (Vicia sativa) and hairy vetch (Vicia villosa).

This study examined the …

The Effects Of Living Mulches On Organic, Reduced-Till Broccoli Growth And Management, Sarah Elizabeth Broughton

Masters Theses

Conservation tillage programs have been successfully implemented for many agronomic cropping systems, but adoption of reduced tillage for vegetable crops has been slow. As many conventionally managed conservation tillage programs rely on synthetic herbicides for clean cultivation, alternative methods must be devised to suppress weed pressure and reduce reliance on mechanical cultivation to aid in the development of reduced tillage programs for organic cropping system. Strip tillage is a reduced tillage method that is well suited to vegetable crop production, and the utilization of cover crops and living mulches between rows provides a viable weed management option for organic systems. …

Control Of Glyphosate Resistant Horseweed (Conyza Canadensis) With Saflufenacil And Tank-Mixture Partners., Brock Steven Waggoner

Masters Theses

Field and labratory studies were conducted to determine the efficacy of saflufenacil alone and with mixture partners for burndown. Field studies were conducted in 2009 and 2010 to evaluate saflufenacil in mixtures with glyphosate, glufosinate, or paraquat for control of glyphosate-resistant (GR) horseweed prior to planting cotton. Saflufenacil and saflufenacil mixtures were applied 7 days before planting (DBP). Saflufenacil at 25 and 50 g ai ha-1 in mixture with all three non-selective herbicides provided similar GR horseweed control when compared to the current standard of glyphosate plus dicamba. Control of GR horseweed was also not different at the 25 and …