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Full-Text Articles in Life Sciences

Estimation And Testing Of Gene Expression Heterosis, Tieming Ji, Peng Liu, Dan Nettleton Jun 2019

Estimation And Testing Of Gene Expression Heterosis, Tieming Ji, Peng Liu, Dan Nettleton

Dan Nettleton

Heterosis, also known as the hybrid vigor, occurs when the mean phenotype of hybrid offspring is superior to that of its two inbred parents. The heterosis phenomenon is extensively utilized in agriculture though the molecular basis is still unknown. In an effort to understand phenotypic heterosis at the molecular level, researchers have begun to compare expression levels of thousands of genes between parental inbred lines and their hybrid offspring to search for evidence of gene expression heterosis. Standard statistical approaches for separately analyzing expression data for each gene can produce biased and highly variable estimates and unreliable tests of heterosis. …


Genome-Wide Identification And Molecular Characterization Of Citrus Unshiu Wrkytranscription Factors In Satsuma Mandarin: Clues For Putative Involvement In Cell Growth,Fruit Ripening, And Stress Response, Mariem Ayadi, Rim Mzid, Rayda Ben Ayed, Najla Kharrat, Henda Merchaoui, Ahmed Rebai, Mohsen Hanana Jan 2019

Genome-Wide Identification And Molecular Characterization Of Citrus Unshiu Wrkytranscription Factors In Satsuma Mandarin: Clues For Putative Involvement In Cell Growth,Fruit Ripening, And Stress Response, Mariem Ayadi, Rim Mzid, Rayda Ben Ayed, Najla Kharrat, Henda Merchaoui, Ahmed Rebai, Mohsen Hanana

Turkish Journal of Agriculture and Forestry

WRKY transcription factors, being involved in several biological roles, stimulus signaling, and stress response, could be interesting candidates to prospect, particularly in the Citrus unshiu (Satsuma mandarin) genome. Our approach, mainly based on computational analyses, led to the identification of 51 CiuWRKYs followed by their molecular characterization. Syntenic relationship analysis showed significant bias towards sweet orange and Arabidopsis chromosomes. Analyses of promoter region showed that CiuWRKY1 modulates gene transcription through W-box site targeting. The deduced CiuWRKY1 protein was moderately hydrophobic. A comparative in silico analysis among Arabidopsis thaliana and Citrus sinensis WRKYs allowed us to designate orthologous genes. Lacking available …