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Full-Text Articles in Life Sciences
‘Forrest’ Resistance To The Soybean Cyst Nematode Is Bigenic: Saturation Mapping Of The Rhg1 And Rhg4 Loci, K. Meksem, P. Pantazopoulos, V. N. Njiti, D. L. Hyten, P. R. Arelli, D. A. Lightfoot
‘Forrest’ Resistance To The Soybean Cyst Nematode Is Bigenic: Saturation Mapping Of The Rhg1 And Rhg4 Loci, K. Meksem, P. Pantazopoulos, V. N. Njiti, D. L. Hyten, P. R. Arelli, D. A. Lightfoot
Department of Agronomy and Horticulture: Faculty Publications
Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv ‘Forrest’ is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived from a cross of Forrest × Essex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg …
High-Throughput Genotyping For A Polymorphism Linked To Soybean Cyst Nematode Resistance Gene Rhg4 By Using TaqmanTm Probes, Khalid Meksem, Eliza Ruben, David L. Hyten, Michael E. Schmidt, David A. Lightfoot
High-Throughput Genotyping For A Polymorphism Linked To Soybean Cyst Nematode Resistance Gene Rhg4 By Using TaqmanTm Probes, Khalid Meksem, Eliza Ruben, David L. Hyten, Michael E. Schmidt, David A. Lightfoot
Department of Agronomy and Horticulture: Faculty Publications
An individual soybean breeder can generate over one hundred thousand new genotypes each year. The efficiency of selection in these populations could be improved if these genotypes were effectively screened with one DNA marker that identified an important gene, and if laboratory throughput was high and costs were low. Our aim was to develop a rapid genotyping procedure for resistance to the soybean cyst nematode. A high-throughput genotyping method was developed with fluorogenic probes to distinguish between two insertion polymorphisms in alleles of an AFLP marker that is located about 50 kbp from the Rhg4 gene candidate. The assay uses …
Genetic Analysis Of Kafirins And Their Phenotypic Correlations With Feed Quality Traits, In Vitro Digestibility, And Seed Weight In Grain Sorghum, C. Hicks, S. R. Bean, G. L. Lookhart, Jeffrey F. Pedersen, K. D. Kofoid, M. R. Tuinstra
Genetic Analysis Of Kafirins And Their Phenotypic Correlations With Feed Quality Traits, In Vitro Digestibility, And Seed Weight In Grain Sorghum, C. Hicks, S. R. Bean, G. L. Lookhart, Jeffrey F. Pedersen, K. D. Kofoid, M. R. Tuinstra
Department of Agronomy and Horticulture: Faculty Publications
Twenty-three entries of grain sorghum (Sorghum bicolor (L.) Moench), including eight inbred lines (five males and three females) and 15 hybrids, were evaluated to determine the proportion of γ, αII, and β-αI-kafirins and their association with contents of crude protein, fat, and starch; protein digestibility; in vitro dry matter disappearance; and seed weight. The male lines included three normal-seeded lines (TX2737, TX435, and P954063) and two large-seeded lines (Eastin1 and PL-1). Female lines consisted of three common U.S. seed parent lines (Wheatland, Redlan, and SA3042). The lines and their hybrids were grown under dryland conditions at two locations in …
Conversion Of Aflp Bands Into High-Throughput Dna Markers, K. Meksem, E. Ruben, D. L. Hyten, K. Triwitayakorn, D. A. Lightfoot
Conversion Of Aflp Bands Into High-Throughput Dna Markers, K. Meksem, E. Ruben, D. L. Hyten, K. Triwitayakorn, D. A. Lightfoot
Department of Agronomy and Horticulture: Faculty Publications
The conversion of AFLP bands into polymorphic sequence-tagged-site (STS) markers is necessary for high-throughput genotype scoring. Technical hurdles that must be overcome arise from genome complexity (particularly sequence duplication), from the low-molecular- weight nature of the AFLP bands and from the location of the polymorphism within the AFLP band. We generated six STS markers from ten AFLP bands (four AFLPs were from co-dominant pairs of bands) in soybean (Glycine max). The markers were all linked to one of two loci, rhg1 on linkage group G and Rhg4 on linkage group A2, that confer resistance to the soybean cyst nematode (Heterodera …
Genomic Regions That Underlie Soybean Seed Isoflavone Content, K. Meksem, V. N. Njiti, W. J. Banz, M. J. Iqbal, My. M. Kassem, David L. Hyten, J. Yuang, T. A. Winters, D. A. Lightfoot
Genomic Regions That Underlie Soybean Seed Isoflavone Content, K. Meksem, V. N. Njiti, W. J. Banz, M. J. Iqbal, My. M. Kassem, David L. Hyten, J. Yuang, T. A. Winters, D. A. Lightfoot
Department of Agronomy and Horticulture: Faculty Publications
Soy products contain isoflavones (genistein, daidzein, and glycitein)that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL)fr om the cross of ‘Essex’ by ‘Forrest,’ two cultivars that contrast for isoflavone content. Isoflavone content of seeds fromeach RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The …
Seed Physiology, Production & Technology: Germination, Emergence, And Yield Of 20 Plant-Color, Seed-Color Near-Isogenic Lines Of Grain Sorghum, Jeffrey F. Pedersen, J. J. Toy
Seed Physiology, Production & Technology: Germination, Emergence, And Yield Of 20 Plant-Color, Seed-Color Near-Isogenic Lines Of Grain Sorghum, Jeffrey F. Pedersen, J. J. Toy
Department of Agronomy and Horticulture: Faculty Publications
Although there is growing demand for sorghum [Sorghum bicolor (L.) Moench] with white seed and tan plant color, there is limited information on the overall agronomic fitness of sorghum with these characters. A set of experiments was conducted to evaluate the combined effects of plant color and seed color on sorghum germination, emergence, and agronomic performance. Twenty near-isogenic lines with red seed/tan plant (RT), red seed/purple plant (RP), white seed/ tan plant (WT), white seed/purple plant (WP) phenotypes were tested under field and laboratory conditions. Plant color X seed color interactions were not significant. Purple plant color phenotypes had higher …