Life Sciences Commons^™

Efficiency In Thin-Film Liquid System For Hosta Micropropagation, Jeffrey Adelberg

Publications

Three varieties of Hosta (‘Striptease’, ‘Minuteman’ and ‘Stiletto’) at four densities (40, 80, 120 and 200 explants per litre) were micropropagated on semi-solid agar and a thin-film liquid system with intermittent wetting of plant tissue. The mechanics of wetting by a small wave front required a larger rectangular vessel (11 × 27=297 cm ²) compared to the common cylindrical baby food jar (18 cm ²). Plants multiplied more rapidly in the agitated thin-film system than on agar. Lower plant densities increased rates of multiplication in liquid, but had little or no effect on multiplication rate on agar. Increasing …