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Laboratory Study Of Responses Of Anuran Amphibians To Roundup¨ Exposures: Experimental Design And Role Of Sediment, Latice Fuentes

All Theses

The contribution of incidental exposures of Roundup¨ herbicide to amphibian declines can be evaluated with carefully designed laboratory tests. Sensitive, sentinel species of larval anurans were exposed to two formulations: the original formulation of Roundup¨ and Roundup WeatherMax¨ in 96-hour acute, aqueous and water-sediment tests. An experimental design was developed that considered and held constant relevant laboratory conditions which produced precise, replicable data. Experimental design factors decreased variability and increased precision by considering and incorporating the following: 1) range-finding tests; 2) reference toxicity tests; 3) the relative sensitivity of the species tested; 4) differences in formulation; 5) the number of …