Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Apoplastic Redox Metabolism: Synergistic Phenolic Oxidation And A Novel Oxidative Burst, C. Jacyn Baker, Daniel P. Roberts, Norton M. Mock, Bruce D. Whitaker, Kenneth L. Deahl, Andrey A. Aver'yanov
Apoplastic Redox Metabolism: Synergistic Phenolic Oxidation And A Novel Oxidative Burst, C. Jacyn Baker, Daniel P. Roberts, Norton M. Mock, Bruce D. Whitaker, Kenneth L. Deahl, Andrey A. Aver'yanov
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The plant apoplast is an important mediator of communication between the cell cytoplasm and its surroundings. Plant cell suspensions offer a convenient model system to gain insight into apoplastic physiology. Here, we describe a novel phenomenon that took place when two naturally occurring phenolics were added together to either soybean or tobacco cell suspensions. Acetosyringone (AS) and/or hydroxyacetophenone (HAP), phenolics found in the extracellular/apoplast of tobacco cells, were added to soybean or tobacco cell suspensions undergoing an oxidative burst. Individually, AS appeared to be utilized as a typical peroxidase substrate to scavenge hydrogen peroxide, while HAP was utilized at a …
Induction Of Redox Sensitive Extracellular Phenolics During Plant–Bacterial Interactions, C. Jacyn Baker, Bruce D. Whitaker, Daniel P. Roberts, Norton M. Mock, Clifford P. Rice, Kenneth L. Deahl, Andrey A. Aver'yanov
Induction Of Redox Sensitive Extracellular Phenolics During Plant–Bacterial Interactions, C. Jacyn Baker, Bruce D. Whitaker, Daniel P. Roberts, Norton M. Mock, Clifford P. Rice, Kenneth L. Deahl, Andrey A. Aver'yanov
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
This study focuses on the transient and complex nature of phenolics that accumulate in the extracellular environment of plant suspension cells during the first few hours of the interaction between these plant cells and bacterial pathogens. Using suspension cells of Nicotiana tabacum we identified four acetophenones and four hydroxycinnamic acid amides that accumulate in this extracellular environment. Treatment of the suspension cells with isolates of the plant pathogen Pseudomonas syringae or heat-killed bacteria increased elicitation of extracellular phenolics and changed the composition of the compounds that accumulated. These phenolics were sensitive to oxidative stress; when suspension cells were treated with …
Involvement Of Acetosyringone In Plant–Pathogen Recognition, C. Jacyn Baker, Norton M. Mock, Bruce D. Whitaker, Daniel P. Roberts, Clifford P. Rice, Kenneth L. Deahl, Andrey A. Aver'yanov
Involvement Of Acetosyringone In Plant–Pathogen Recognition, C. Jacyn Baker, Norton M. Mock, Bruce D. Whitaker, Daniel P. Roberts, Clifford P. Rice, Kenneth L. Deahl, Andrey A. Aver'yanov
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
In this study, acetosyringone was identified as one of the major extracellular phenolics in tobacco suspension cells and was shown to have bioactive properties that influence early events in plant–bacterial pathogenesis. In our model system, tobacco cell suspensions treated with bacterial isolate Pseudomonas syringae WT (HR+) undergo a resistant interaction characterized by a burst in oxygen uptake several hours after inoculation. When the extracellular concentration of acetosyringone in tobacco cell suspensions was supplemented with exogenous acetosyringone, the burst in oxygen uptake occurred as much as 1.5 h earlier. The exogenous acetosyringone had no effect on tobacco suspensions undergoing susceptible interactions …