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Full-Text Articles in Life Sciences

Strategic Switchgrass (Panicum Virgatum) Production Within Row Cropping Systems: Regional-Scale Assessment Of Soil Erosion Loss And Water Runoff Impacts, Enheng Wang, R. M. Cruse, Bharat Sharma-Acharya, Daryl E. Herzmann, Brian K. Gelder, David E. James, Dennis C. Flanagan, Humberto Blanco-Canqui, Robert B. Mitchell, David Laird Jan 2020

Strategic Switchgrass (Panicum Virgatum) Production Within Row Cropping Systems: Regional-Scale Assessment Of Soil Erosion Loss And Water Runoff Impacts, Enheng Wang, R. M. Cruse, Bharat Sharma-Acharya, Daryl E. Herzmann, Brian K. Gelder, David E. James, Dennis C. Flanagan, Humberto Blanco-Canqui, Robert B. Mitchell, David Laird

Department of Agronomy and Horticulture: Faculty Publications

A strong need exists for tools to assess the efficacy of conservation practices across large regions supporting informed policy decisions that may lead to better soil and water conservation while optimizing agricultural production options. Perennial warm-season grasses (WSGs) such as switchgrass (Panicum virgatum), can be grown on marginally productive and/or environmentally sensitive lands to meet growing bioenergy demands while reducing water runoff and soil erosion compared to current row crop systems. Quantifying the soil and water conservation effects of WSG when strategically placed on the landscape would help support decisions favoring both economic and environmental benefits. We used the Daily …


Soil Carbon Increased By Twice The Amount Of Biochar Carbon Applied After 6 Years: Field Evidence Of Negative Priming, Humberto Blanco-Canqui, David A. Laird, Emily A. Heaton, Samuel Rathke, Bharat Sharma Acharya Jan 2020

Soil Carbon Increased By Twice The Amount Of Biochar Carbon Applied After 6 Years: Field Evidence Of Negative Priming, Humberto Blanco-Canqui, David A. Laird, Emily A. Heaton, Samuel Rathke, Bharat Sharma Acharya

Department of Agronomy and Horticulture: Faculty Publications

Applying biochar to agricultural soils has been proposed as a means of sequester-ing carbon (C) while simultaneously enhancing soil health and agricultural sustain-ability. However, our understanding of the long-term effects of biochar and annual versus perennial cropping systems and their interactions on soil properties under field conditions is limited. We quantified changes in soil C concentration and stocks, and other soil properties 6 years after biochar applications to corn (Zea mays L.) and dedicated bioenergy crops on a Midwestern US soil. Treatments were as fol-lows: no-till continuous corn, Liberty switchgrass (Panicum virgatum L.), and low-diversity prairie grasses, 45% big bluestem …


Rust And Viral Mosaic Diseases In Biofuel Switchgrass, Anthony A. Muhle Aug 2019

Rust And Viral Mosaic Diseases In Biofuel Switchgrass, Anthony A. Muhle

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Switchgrass (Panicum virgatum L.) is a perennial warm-season monocot that is indigenous to locations in North America east of the Rocky Mountains, and is considered a model grass for biofuel feedstock production. As switchgrass production increases, diseases pose a potential threat to biomass production and ethanol extraction. The two predominant switchgrass diseases in Nebraska are rust caused by Puccinia spp. and a viral mosaic disease caused by Panicum mosaic virus (PMV) and its associated Satellite panicum mosaic virus (SPMV). In this thesis, one study determined how SPMV affects PMV infection and systemic spread in two populations of switchgrass at different …


Impact Of Drought On Chemical Composition And Sugar Yields From Dilute-Acid Pretreatment And Enzymatic Hydrolysis Of Miscanthus, A Tall Fescue Mixture, And Switchgrass, Amber Hoover, Rachel Emerson, Allison Ray, Daniel Stevens, Sabrina Morgan, Marnie Cortez, Robert Kallenbach, Matthew Sousek, Rodney Farris, Dayna Daubaras Jan 2018

Impact Of Drought On Chemical Composition And Sugar Yields From Dilute-Acid Pretreatment And Enzymatic Hydrolysis Of Miscanthus, A Tall Fescue Mixture, And Switchgrass, Amber Hoover, Rachel Emerson, Allison Ray, Daniel Stevens, Sabrina Morgan, Marnie Cortez, Robert Kallenbach, Matthew Sousek, Rodney Farris, Dayna Daubaras

Department of Agronomy and Horticulture: Faculty Publications

Environmental factors like drought impact the quality of biomass entering a bioconversion process. Drought often reduces the sugar content in lignocellulosic biomass, which could have economic impacts, particularly when compounded with losses in dry biomass yield; however, the effects on conversion efficiency are not completely understood. This study investigated how drought may impact biomass composition and sugar yields from dilute-acid pretreatment and enzymatic hydrolysis of Miscanthus, a tall fescue mixture, and switchgrass from Nebraska, Missouri, and Oklahoma, respectively, grown as part of Regional Feedstock Partnership field trials. Samples were grown and harvested in 2010 during non-drought conditions and in …


Productivity Of Well-Watered Panicum Virgatum Does Not Increase With Co2 Enrichment, Philip A. Fay, H. Wayne Polley, Virginia L. Jin, Michael J. Aspinwall Oct 2012

Productivity Of Well-Watered Panicum Virgatum Does Not Increase With Co2 Enrichment, Philip A. Fay, H. Wayne Polley, Virginia L. Jin, Michael J. Aspinwall

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aims Rising atmospheric CO2 has been shown to increase aboveground net primary productivity (ANPP) in water-limited perennial grasslands, in part by reducing stomatal conductance and transpiration, thereby reducing depletion of soil moisture. However, the benefits of CO2 enrichment for ANPP will vary with soil type and may be reduced if water limitation is low. Little is known about CO2 effects on ANPP of Panicum virgatum, a perennial C4 tallgrass and potential bioenergy crop. We hypothesized that if water limitation is minimized, (i) CO2 enrichmentwould not increase P. virgatum ANPP because photosynthetic rates of this C …


Carbon Exchange By Establishing Biofuel Crops In Central Illinois, Marcelo Zeri, Kristina Anderson-Teixeira, George Hickman, Michael Masters, Evan Delucia, Carl J. Bernacchi Jan 2011

Carbon Exchange By Establishing Biofuel Crops In Central Illinois, Marcelo Zeri, Kristina Anderson-Teixeira, George Hickman, Michael Masters, Evan Delucia, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Perennial grass bio-fuels may contribute to long-term carbon sequestration in soils, thereby providing a broad range of environmental benefits. To quantify those benefits, the carbon balance was investigated over three perennial grass bio-fuel crops – miscanthus (Miscanthus×giganteus), switch-grass (Panicum virgatum) and a mixture of native prairie plants – and a row crop control (maize–maize–soy) in Central Illinois, USA, during the establishment phase of the perennial grasses (2008–2011). The eddy covariance technique was used to calculate fluxes of carbon dioxide and energy balance components, such as latent and sensible heat fluxes. Whereas maize attained the highest maximal …


Field-Scale Soil Property Changes Under Switchgrass Managed For Bioenergy, Marty R. Schmer, M. A. Liebig, K. P. Vogel, Robert B. Mitchell Jan 2011

Field-Scale Soil Property Changes Under Switchgrass Managed For Bioenergy, Marty R. Schmer, M. A. Liebig, K. P. Vogel, Robert B. Mitchell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The capacity of perennial grasses to affect change in soil properties is well documented but information on switchgrass (Panicum virgatum L.) managed for bioenergy is limited. An on-farm study (10 fields) in North Dakota, South Dakota, and Nebraska was sampled before switchgrass establishment and after 5 years to determine changes in soil bulk density (SBD), pH, soil phosphorus (P), and equivalent mass soil organic carbon (SOC). Changes in SBD were largely constrained to near-surface depths (0–0.05 m). SBD increased (0–0.05 m) at the Nebraska locations (mean=0.16 Mgm-3), while most South Dakota and North Dakota locations showed declines …


Switchgrass Contains Two Cinnamyl Alcohol Dehydrogenases Involved In Lignin Formation, Aaron J. Saathoff, Christian M. Tobias, Scott E. Sattler, Eric J. Haas, Paul Twigg, Gautam Sarath Jan 2011

Switchgrass Contains Two Cinnamyl Alcohol Dehydrogenases Involved In Lignin Formation, Aaron J. Saathoff, Christian M. Tobias, Scott E. Sattler, Eric J. Haas, Paul Twigg, Gautam Sarath

Department of Agronomy and Horticulture: Faculty Publications

Lignin content of switchgrass (Panicum virgatum L.), a bioenergy species, is a critical determinant of biomass quality since it can negatively impact conversion of biomass into liquid fuels via biochemical platforms. Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme in lignin biosynthesis. Here, we have shown that cv. Kanlow switchgrass contains at least two closely related CAD genes (PviCAD1 and PviCAD2) that code for proteins containing highly conserved domains and residues that identify them as bona fide CADs. Both recombinant proteins displayed substrate kinetics consistent with their presumed role in cell wall lignification. Proteomic and immunoblotting detected …


Herbicides For Establishing Switchgrass In The Central And Northern Great Plains, Robert B. Mitchell, Kenneth P. Vogel, John Berdahl, Robert A. Masters Jan 2010

Herbicides For Establishing Switchgrass In The Central And Northern Great Plains, Robert B. Mitchell, Kenneth P. Vogel, John Berdahl, Robert A. Masters

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Weed interference limits switchgrass (Panicum virgatum L.) establishment from seed. Our objectives were to determine the effect of selected post-plant, preemergence herbicides on stand establishment and subsequent biomass yields of adapted upland switchgrass cultivars grown in three environments in the Central and Northern Great Plains. A separate experiment was conducted in eastern Nebraska to determine if there were any differences among switchgrass ecotypes for herbicide tolerance to the optimal herbicide combination. Herbicides applied immediately after planting were different concentrations of atrazine [Aatrex 4L®; 6- chloro-N-ethyl-N′-(1-methylethyl)-1,3,5-triazine-2,4-diamine], quinclorac (Paramount®; 3,7-Dichloro-8-quinolinecarboxylic acid), atrazine+quinclorac, imazapic …


Monitoring And Analyzing Process Streams Towards Understanding Ionic Liquid Pretreatment Of Switchgrass (Panicum Virgatum L.), Rohit Arora, Chithra Manisseri, Chenlin Li, Markus D. Ong, Henrik Vibe Scheller, Kenneth P. Vogel, Blake A. Simmons, Seema Singh Jan 2010

Monitoring And Analyzing Process Streams Towards Understanding Ionic Liquid Pretreatment Of Switchgrass (Panicum Virgatum L.), Rohit Arora, Chithra Manisseri, Chenlin Li, Markus D. Ong, Henrik Vibe Scheller, Kenneth P. Vogel, Blake A. Simmons, Seema Singh

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fundamental understanding of biomass pretreatment and its influence on saccharification kinetics, total sugar yield, and inhibitor formation is essential to develop efficient next-generation biofuel strategies, capable of displacing fossil fuels at a commercial level. In this study, we investigated the effect of residence time and temperature during ionic liquid (IL) pretreatment of switchgrass using 1-ethyl-3-methyl imidazolium acetate. The primary metrics of pretreatment performance are biomass delignification, xylan and glucan depolymerization, porosity, surface area, cellulase kinetics, and sugar yields. Compositional analysis and quantification of process streams of saccharides and lignin demonstrate that delignification increases as a function of pretreatment temperature and …


Spatial And Temporal Effects On Switchgrass Stands And Yield In The Great Plains, Marty R. Schmer, R. B. Mitchell, K. P. Vogel, Walter H. Schacht, David B. Marx Jan 2009

Spatial And Temporal Effects On Switchgrass Stands And Yield In The Great Plains, Marty R. Schmer, R. B. Mitchell, K. P. Vogel, Walter H. Schacht, David B. Marx

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass (Panicum virgatum L.) is being developed into a perennial, herbaceous, cellulosic feedstock crop for use in temperate regions of the USA. Information on spatial and temporal variation for stands and biomass yield among and within fields in large agroecoregions is not available. Spatial and temporal variation information is needed to model feedstock availability for biorefineries. In this 5-yr study, the spatial and temporal variation for biomass yield and stands was determined among and within 10 fields located in North Dakota, South Dakota, and Nebraska. Switchgrass fields were managed for bioenergy from 2000 to 2004 for the Nebraska locations …


Opportunities And Roadblocks In Utilizing Forages And Small Grains For Liquid Fuels, Gautam Sarath, Robert B. Mitchell, S. E. Sattler, Deanna L. Funnell, Jeffrey F. Pedersen, Robert A. Graybosch, Kenneth P. Vogel Jan 2008

Opportunities And Roadblocks In Utilizing Forages And Small Grains For Liquid Fuels, Gautam Sarath, Robert B. Mitchell, S. E. Sattler, Deanna L. Funnell, Jeffrey F. Pedersen, Robert A. Graybosch, Kenneth P. Vogel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This review focuses on the potential advantages and disadvantages of forages such as switchgrass (Panicum virgatum), and two small grains: sorghum (Sorghum bicolor), and wheat (Triticum aesitvum), as feedstocks for biofuels. It highlights the synergy provided by applying what is known from forage digestibility and wheat and sorghum starch properties studies to the biofuels sector. Opportunities therefore, exist to improve biofuel qualities in these crops via genetics and agronomics. In contrast to cereal crops, switchgrass still retains tremendous exploitable genetic diversity, and can be specifically improved to Wt a particular agronomic, management, and conversion …


Nitric Oxide Accelerates Seed Germination In Warm-Season Grasses, Gautam Sarath, Paul C. Bethke, Russell Jones, Lisa M. Baird, Guichuan Hou, Robert B. Mitchell May 2006

Nitric Oxide Accelerates Seed Germination In Warm-Season Grasses, Gautam Sarath, Paul C. Bethke, Russell Jones, Lisa M. Baird, Guichuan Hou, Robert B. Mitchell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The nitric oxide (NO) donor sodium nitroprusside (SNP) significantly promoted germination of switchgrass (Panicum virgatum L. cv Kanlow) in the light and in the dark at 25°C, across a broad range of concentrations. SNP also promoted seed germination in two other warm-season grasses. A chemical scavenger of NO inhibited germination and blocked SNP stimulation of seed germination. The phenolic (+)-catechin acted synergistically with SNP and nitrite in promoting seed germination. Acidified nitrite, an alternate NO donor also significantly stimulated seed germination. Interestingly, sodium cyanide, potassium ferricyanide and potassium ferrocyanide at 200 lM strongly enhanced seed germination as well, whereas …