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Full-Text Articles in Life Sciences
Dual Biological Control: Characterization Of Fungi And Bacteria To Control Granary Weevil And Fungal Pathogens Of Stored Grain, Gülçin Ercan
Dual Biological Control: Characterization Of Fungi And Bacteria To Control Granary Weevil And Fungal Pathogens Of Stored Grain, Gülçin Ercan
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Cereals are main food sources for humans and animals. However, during storage, cereal grains can be infested by insects and fungi. One of the most important insect storage pests is Sitophilus granarius (L., Coleoptera: Curculionidae). Adults and larvae can cause serious grain losses. In addition to insect pests, fungal pathogens may also invade the grain and cause economic loss, including contamination with mycotoxins, which threaten mammal health by causing serious disease. The most common mycotoxigenic grain fungi are species that belong to the genera Fusarium, Aspergillus and Penicillium. Currently, the most commonly used management strategies for insect and …
Molecular Gut-Content Analysis Reveals High Frequency Of Helicoverpa Zea (Lepidoptera: Noctuidae) Consumption By Orius Insidiosus (Hemiptera: Anthocoridae) In Sweet Corn, Julie A. Peterson, E. C. Burkness, James D. Harwood, W. D. Hutchison
Molecular Gut-Content Analysis Reveals High Frequency Of Helicoverpa Zea (Lepidoptera: Noctuidae) Consumption By Orius Insidiosus (Hemiptera: Anthocoridae) In Sweet Corn, Julie A. Peterson, E. C. Burkness, James D. Harwood, W. D. Hutchison
Department of Entomology: Faculty Publications
Management of corn earworm Helicoverpa zea in sweet corn grown for processing can be challenging due to the lack of effective transgenic and chemical control options. However, biological control by generalist predators can provide a significant impact on pests in this cropping system. One of the most ubiquitous predators of H. zea and other lepidopterans is the insidious flower bug, Orius insidiosus. This small hemipteran has been observed as an important mortality agent of H. zea in several cropping systems, but the strength of the trophic connection between these species has not been documented in sweet corn. Molecular gut-content …
Outcrossing And Crossbreeding Recovers Deteriorated Traits In Laboratory Cultured Steinernema Carpocapsae Nematodes, John M. Chaston, Adler R. Dillman, David I. Shapiro-Ilan, Anwar L. Bilgrami, Randy Gaugler, Keith R. Hopper, Byron J. Adams
Outcrossing And Crossbreeding Recovers Deteriorated Traits In Laboratory Cultured Steinernema Carpocapsae Nematodes, John M. Chaston, Adler R. Dillman, David I. Shapiro-Ilan, Anwar L. Bilgrami, Randy Gaugler, Keith R. Hopper, Byron J. Adams
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The nematode Steinernema carpocapsae infects and kills many pest insects in agro-ecosystems and is commonly used in biocontrol of these pests. Growth of the nematodes prior to distribution for biocontrol commonly results in deterioration of traits that are essential for nematode persistence in field applications. To better understand the mechanisms underlying trait deterioration of the efficacy of natural parasitism in entomopathogenic nematodes, we explored the maintenance of fitness related traits including reproductive capacity, heat tolerance, virulence to insects and ‘tail standing’ (formerly called nictation) among laboratory-cultured lines derived from natural, randomly mating populations of S. carpocapsae. Laboratory cultured nematode …
Classical Biological Control For The Protection Of Natural Ecosystems, R.G. Van Driesche, R I. Carruthers, T. Center, M.S. Hoddle, J. Hough-Goldstein, L. Morin, L. Smith, D.L. Wagner, B. Blossey, V. Brancatini, R. Casagrande, C.E. Causton, J.A. Coetzee, J. Cuda, J. Ding, S.V. Fowler, J. H. Frank, R. Fuester, J. A. Goolsby, M. Grodowitz, T.A. Heard, M.P. Hill, J.H. Hoffmann, J. Huber, M. Julien, M.T.K. Kairo, M. Kenis, P. Mason, J. Medal, R. Messing, R. Miller, A. Moore, P. Neuenschwander, R. Newman, H. Norambuena, W.A. Palmer, R. Pemberton, A. Perez-Panduro, P.D. Pratt, M. Rayamajhi, S. Salom, D. Sands, S. Schooler, M. Schwarzländer, A. Sheppard, R. Shaw, P.W. Tipping, R.D. Van Klinken
Classical Biological Control For The Protection Of Natural Ecosystems, R.G. Van Driesche, R I. Carruthers, T. Center, M.S. Hoddle, J. Hough-Goldstein, L. Morin, L. Smith, D.L. Wagner, B. Blossey, V. Brancatini, R. Casagrande, C.E. Causton, J.A. Coetzee, J. Cuda, J. Ding, S.V. Fowler, J. H. Frank, R. Fuester, J. A. Goolsby, M. Grodowitz, T.A. Heard, M.P. Hill, J.H. Hoffmann, J. Huber, M. Julien, M.T.K. Kairo, M. Kenis, P. Mason, J. Medal, R. Messing, R. Miller, A. Moore, P. Neuenschwander, R. Newman, H. Norambuena, W.A. Palmer, R. Pemberton, A. Perez-Panduro, P.D. Pratt, M. Rayamajhi, S. Salom, D. Sands, S. Schooler, M. Schwarzländer, A. Sheppard, R. Shaw, P.W. Tipping, R.D. Van Klinken
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Of the 70 cases of classical biological control for the protection of nature found in our review, there were fewer projects against insect targets (21) than against invasive plants (49), in part, because many insect biological control projects were carried out against agricultural pests, while nearly all projects against plants targeted invasive plants in natural ecosystems. Of 21 insect projects, 81% (17) provided benefits to protection of biodiversity, while 48% (10) protected products harvested from natural systems, and 5% (1) preserved ecosystem services, with many projects contributing to more than one goal. In contrast, of the 49 projects against invasive …
Field Applications Of Entomopathogenic Fungi Beauveria Bassiana And Metarhizium Anisopliae F52 (Hypocreales: Clavicipitaceae) For The Control Of Ixodes Scapularis (Acari: Ixodidae), Kirby C. Stafford Iii, Sandra A. Allan
Field Applications Of Entomopathogenic Fungi Beauveria Bassiana And Metarhizium Anisopliae F52 (Hypocreales: Clavicipitaceae) For The Control Of Ixodes Scapularis (Acari: Ixodidae), Kirby C. Stafford Iii, Sandra A. Allan
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Two commercial formulations of Beauveria bassiana (Balsamo-Crivelli) Vuillemin were applied to residential sites in Old Lyme, CT, for the control of nymphs of the blacklegged tick, Ixodes scapularis, in 1999 and 2000. The pyrethroid bifenthrin was applied to other premises for comparison with B. bassiana. A wood chip barrier was installed and maintained at six of the treated properties. In 1999, control of I. scapularis nymphs ranged from 74.5 to 83.0% on lawns without wood chips and from 88.9 to 90% on lawns with wood chip barriers. As a control check, no ticks (n= 23) collected …
Efficiency And Establishment Of Three Introduced Parasitoids Of The Mealybug Paracoccus Marginatus (Hemiptera: Pseudococcidae), Kaushalya G. Amarasekare, Catharine M. Mannion, Nancy D. Epsky
Efficiency And Establishment Of Three Introduced Parasitoids Of The Mealybug Paracoccus Marginatus (Hemiptera: Pseudococcidae), Kaushalya G. Amarasekare, Catharine M. Mannion, Nancy D. Epsky
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Three introduced parasitoids Acerophagus papayae, Anagyrus loecki, and Pseudleptomastix mexicana of the mealybug Paracoccus marginatus have been released in Miami-Dade and Broward counties (Florida) in 2003. Efficiency and establishment of these previously introduced parasitoids to control P. marginatus were measured in 2005 and 2006, at three locations in Homestead (Miami-Dade). Mealybug populations were initially established on three branches (per plant) of 10 hibiscus plants inside closed-sleeve cages. The three treatments, closed, open, and no-cage environments were applied to the three established mealybug populations on each plant. The number of mealybugs and natural enemies was monitored in all treatments. …
Where To Sample? Ecological Implications Of Sampling Strata In Determining Abundance And Impact Of Natural Enemies Of The Coffee Berry Borer, Hypothenemus Hampei, Juliana Jaramillo, Adenirin Chabi-Olaye, Christian Borgemeister, Charles Kamonjo, Hans-Michael Poehling, Fernando E. Vega
Where To Sample? Ecological Implications Of Sampling Strata In Determining Abundance And Impact Of Natural Enemies Of The Coffee Berry Borer, Hypothenemus Hampei, Juliana Jaramillo, Adenirin Chabi-Olaye, Christian Borgemeister, Charles Kamonjo, Hans-Michael Poehling, Fernando E. Vega
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Several parasitoids of African origin have been introduced to coffee producing areas of the Americas and Asia as biological control agents of the coffee berry borer (CBB) Hypothenemus hampei (Coleoptera: Curculionidae). These parasitoids have become established in the field but their effect on the CBB has been limited. A two-year field study in Western Kenya has found Prorops nasuta (Hymenoptera: Bethylidae) to be the predominant parasitoid emerging from CBB-infested coffee berries collected on coffee trees or from the ground. P. nasuta comprises more than 75% of the total natural enemies collected. The density of P. nasuta was 90% higher in …
Entomopathogenic Fungal Endophytes, Fernando E. Vega, Francisco Posada, M. Catherine Aime, Monica Pava-Ripoll, Francisco Infante, Stephen A. Rehner
Entomopathogenic Fungal Endophytes, Fernando E. Vega, Francisco Posada, M. Catherine Aime, Monica Pava-Ripoll, Francisco Infante, Stephen A. Rehner
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Fungal endophytes are quite common in nature and some of them have been shown to have adverse effects against insects, nematodes, and plant pathogens.
Our research program is aimed at using fungal endophytes-mediated plant defense as a novel biological control mechanism against the coffee berry borer, the most devastating pest of coffee throughout the world. A survey of fungal endophytes in coffee plants from Hawaii, Colombia, Mexico, and Puerto Rico has revealed the presence of various genera of fungal entomopathogens, including Acremonium, Beauveria, Cladosporium, Clonostachys, and Paecilomyces. Two of these, B. bassiana and Clonostachys rosea …
Physiological Host Range Of Ceratapion Basicorne, A Prospective Biological Control Agent Of Centaurea Solstitialis (Asteraceae), Lincoln Smith
Physiological Host Range Of Ceratapion Basicorne, A Prospective Biological Control Agent Of Centaurea Solstitialis (Asteraceae), Lincoln Smith
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Ceratapion basicorne (Coleoptera: Apionidae) is a weevil native to Europe and western Asia that is being evaluated as a prospective classical biological control agent of Centaurea solstitialis (yellow starthistle) in the United States. Host plant specificity of the insect was evaluated in no-choice oviposition experiments. Feeding on leaf tissue by adult females was highly correlated to oviposition rate, both of which occurred primarily on plants in the tribe Cardueae, and especially those in the monophyletic subtribe Centaureinae. The highest rates of larval development occurred on Ce. solstitialis and Centaurea cyanus (bachelor’s button, garden cornflower), and there was significant development on …
Suppression Of House Flies (Diptera: Muscidae) In Florida Poultry Houses By Sustained Releases Of Muscidifurax Raptorellus And Spalangia Cameroni (Hymenoptera: Pteromalidae), Christopher Geden, Jerome Hogsette
Suppression Of House Flies (Diptera: Muscidae) In Florida Poultry Houses By Sustained Releases Of Muscidifurax Raptorellus And Spalangia Cameroni (Hymenoptera: Pteromalidae), Christopher Geden, Jerome Hogsette
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Weekly releases of Muscidifurax raptorellus Kogan and Legner and Spalangia cameroni Perkins were made for 12 wk after house cleanouts in Florida pullet houses in either spring/summer (May-August) or fall (September-December). Releases were made by weekly placement of 62,500 and 85,000 pupae parasitized by M. raptorellus and S. cameroni, respectively, which produced an average of 79,049 and 32,841 adult female parasitoids per week. House fly (Musca domestica L.) pupal mortality, as measured by sentinel pupae, was about twice as high in the release house (40.2%) as in the two control houses (21.5 and 21.8%) in the summer release. …
Genetic And Hybridization Evidence Confirms That A Geographic Population Of Gonatocerus Morrilli (Hymenoptera: Mymaridae) From California Is A New Species: Egg Parasitoids Of The Glassy-Winged Sharpshooter Homalodisca Coagulata (Homoptera: Cicadellidae), Jesse H. De León, Walker A. Jones, Mamoudou Sétamou, David J. W. Morgan
Genetic And Hybridization Evidence Confirms That A Geographic Population Of Gonatocerus Morrilli (Hymenoptera: Mymaridae) From California Is A New Species: Egg Parasitoids Of The Glassy-Winged Sharpshooter Homalodisca Coagulata (Homoptera: Cicadellidae), Jesse H. De León, Walker A. Jones, Mamoudou Sétamou, David J. W. Morgan
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
We investigated the differentiation and reproductive isolation among different geographic populations of Gonatocerus morrilli, egg parasitoids of the glassy-winged sharpshooter (Homalodisca coagulata), to confirm previous observations that there may exist a cryptic species complex or a new species. Two mitochondrial genes [cytochrome oxidase subunits I (COI) and II (COII)] and the internal transcribed spacer region 2 (ITS2) of several individuals per population were sequenced. G. morrilli populations from Texas (TX), Florida (FL), California (CA), and an outgroup (G. ashmeadi) were analyzed. For comparison, a population from Argentina (G. annulicornis) morphologically similar to G. …
Biocontrol Agents Applied Individually And In Combination For Suppression Of Soilborne Diseases Of Cucumber, Daniel P. Roberts, Scott M. Lohrke, Susan L. F. Meyer, Jeffry S. Buyer, John H. Bowers, C. Jacyn Baker, Wei Li, Jorge T. De Souza, Jack A. Lewis, Soohee Chung
Biocontrol Agents Applied Individually And In Combination For Suppression Of Soilborne Diseases Of Cucumber, Daniel P. Roberts, Scott M. Lohrke, Susan L. F. Meyer, Jeffry S. Buyer, John H. Bowers, C. Jacyn Baker, Wei Li, Jorge T. De Souza, Jack A. Lewis, Soohee Chung
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The soilborne pathogens Rhizoctonia solani, Pythium ultimum, and Meloidogyne incognita can cause severe economic losses to field- and greenhouse-grown cucumber. A collection of bacterial isolates and isolates GL3 and GL21 of Trichoderma virens were screened for suppression of diseases caused by these pathogens. T. virens isolates GL3 and GL21 provided the most effective suppression of damping-off caused by R. solani in greenhouse bioassays. Burkholderia ambifaria BC-F, B. cepacia BC-1, and Serratia marcescens N1-14 also provided significant suppression of R. solani relative to the pathogen check in some experiments. T. virens isolates GL3 and GL21 and S. marcescens isolates …
Risk And Ethics In Biological Control, Ernest S. Delfosse
Risk And Ethics In Biological Control, Ernest S. Delfosse
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
All introduced natural enemies present a degree of risk to nontarget species. Since most biological control programs use relatively host-specific natural enemies, the risk to nontarget species is generally very low, particularly from biological control of weeds, which uses extensively tested and validated host-specificity testing procedures to predict risk. However, many of the published comments about risks of biological control are superficial or misleading, often inappropriately lumping risk from all taxa of agents as “the risk of biological control,” and ignore the potential benefits, rather than dealing with species-by-species risk and benefits. Particularly confounding accurate predictions is the common mixing …
Herbivory Alters Resource Allocation And Compensation In The Invasive Tree Melaleuca Quinquenervia, P. D. Pratt, M. B. Rayamajhi, T. K. Van, T. D. Center, P. W. Tipping
Herbivory Alters Resource Allocation And Compensation In The Invasive Tree Melaleuca Quinquenervia, P. D. Pratt, M. B. Rayamajhi, T. K. Van, T. D. Center, P. W. Tipping
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The Australian native Melaleuca quinquenervia is highly invasive in the Florida Everglades, U.S.A., where it experiences limited competition or herbivory from native species, making it a likely candidate for compensation. The introduced biological control agent Oxyops vitiosa feeds exclusively on the seasonal flushes of developing foliage at branch apices, which represents ~ 15% of the total foliar biomass.
From Metchnikoff To Monsanto And Beyond: The Path Of Microbial Control, Jeffrey C. Lord
From Metchnikoff To Monsanto And Beyond: The Path Of Microbial Control, Jeffrey C. Lord
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
In 125 years since Metchnikoff proposed the use of Metarhizium anisopliae to control the wheat cockchafer and brought about the first field trials, microbial control has progressed from the application of naturalists’ observations to biotechnology and precision delivery. This review highlights major milestones in its evolution and presents a perspective on its current direction. Fungal pathogens, the most eye-catching agents, dominated the early period, but major mycological control efforts for chinch bugs and citrus pests in the US had questionable success, and interest waned. The discoveries of Bacillus popilliae and Bacillus thuringiensis began the era of practical and commercially viable …
Field Assessment Of The Risk Posed By Diorhabda Elongata, A Biocontrol Agent For Control Of Saltcedar (Tamarix Spp.), To A Nontarget Plant, Frankenia Salina, Tom L. Dudley, David J. Kazmer
Field Assessment Of The Risk Posed By Diorhabda Elongata, A Biocontrol Agent For Control Of Saltcedar (Tamarix Spp.), To A Nontarget Plant, Frankenia Salina, Tom L. Dudley, David J. Kazmer
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The biological control program for saltcedar (Tamarix spp.) has led to open releases of a specialist beetle (Chrysomelidae: Diorhabda elongata) in several research locations, but the controversy over potential impacts to native, nontarget plants of the genus Frankenia remains unresolved. To assess the potential for nontarget impacts under Weld conditions, we installed cultivated Frankenia spp. (primarily two forms of Frankenia salina but also including Frankenia jamesii) at locations in Nevada and Wyoming where D. elongata densities and saltcedar defoliation were expected to be very high, so insects would be near starvation with high probability of attacking nontargets …
Habitat Manipulation Of The Apple Orchard Floor To Increase Ground-Dwelling Predators And Predation Of Cydia Pomonella (L.) (Lepidoptera: Tortricidae), Clarissa R. Mathews, Dale G. Bottrell, M. W. Brown
Habitat Manipulation Of The Apple Orchard Floor To Increase Ground-Dwelling Predators And Predation Of Cydia Pomonella (L.) (Lepidoptera: Tortricidae), Clarissa R. Mathews, Dale G. Bottrell, M. W. Brown
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Augmenting generalist predator populations in new apple (Malus domestica Bork) plantings could potentially aid in the establishment of balanced orchard ecosystems that are less susceptible to pest outbreaks. Habitat can be an important factor in retaining predators in a system and can affect predator efficiency. We investigated the potential of increasing a complex of generalist ground-dwelling predators and enhancing biological control in a young 'Golden Delicious' apple orchard through ground habitat manipulation. We modified the orchard floor with four comparative habitat treatments: (1) detritus-rich compost mulch layer, (2) detritus-free synthetic mulch layer, (3) herbicide-treated vegetation thatch, and (4) bare …
Exploratory Surveys In Australia And Asia For Natural Enemies Of Old World Climbing Fern, Lygodium Microphyllum: Lygodiaceae, John A. Goolsby, Anthony D. Wright, Robert W. Pemberton
Exploratory Surveys In Australia And Asia For Natural Enemies Of Old World Climbing Fern, Lygodium Microphyllum: Lygodiaceae, John A. Goolsby, Anthony D. Wright, Robert W. Pemberton
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Lygodium microphyllum, Old World climbing fern, is native to the wet tropics and subtropics of the Old World and an invasive weed in southern Florida. Exploration for natural enemies of this weed was conducted between 1997 and 2002 in Australia, China, India, Indonesia, Japan, Malaysia, New Caledonia, Singapore, Taiwan, Thailand, and Vietnam. Two species of mites and 20 insect species were collected. The eriophyid mite, Floracarus perrepae Knihinicki and Boczek, was the most widely distributed and appeared to cause significant damage to the plant over time. Several unique geographical genotypes of F. perrepae were identified. Other promising candidates for …
Development Of Hydrotaea Aenescens (Diptera: Muscidae) In Manure Of Unweaned Dairy Calves And Lactating Cows, Jerome Hogsette, Robert Farkas, Reginald Coler
Development Of Hydrotaea Aenescens (Diptera: Muscidae) In Manure Of Unweaned Dairy Calves And Lactating Cows, Jerome Hogsette, Robert Farkas, Reginald Coler
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
In laboratory studies performed in the United States and Hungary, the dump fly Hydrotaea aenescens (Wiedemann) was reared successfully in manure of 1- to 8-wk-old dairy calves, and in manure from adult lactating dairy cows. Survival in manure collected from 1-wk-old calves was poor (7.2%), better in manure collected from 2- and 3-wk-old calves (53.5%), and best in manure collected from 4- to 8-wk-old calves (71.4%). Survival in cow manure was slightly lower (47.4%) than that in calf manure. Reasons for different rates of development in the United States and in Hungary, and by calf age are discussed as are …
Strategies For Controlling House Fly Populations Resistant To Cyromazine, Diana C. Crespo, Roberto E. Lecuona, Jerome Hogsette
Strategies For Controlling House Fly Populations Resistant To Cyromazine, Diana C. Crespo, Roberto E. Lecuona, Jerome Hogsette
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The objectives of this study were to compare, from both biological and economic viewpoints, the impact of various control strategies and evaluate their effect on cyromazine-resistant Musca domestica L., and beneficial house fly pupal parasitoids on caged-layer farms (240,000 hens) in Argentina. The strategies evaluated were: chemical, chemical + cultural, and chemical + cultural + biological (integrated management). The products used were: cyromazine 1% and 50%, DDVP, azamethiphos with and without z-9-tricosene, lime, and the parasitoids Spalangia endius Walker and Muscidifurax raptor Girault & Sanders. In the absence of control measures, fly density increased quickly and the average parasitism rate …
Invited Synthesis Paper: Principles And Practices For Managing Rangeland Invasive Plants, Robert A. Masters, Roger Sheley
Invited Synthesis Paper: Principles And Practices For Managing Rangeland Invasive Plants, Robert A. Masters, Roger Sheley
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Invasive plants reduce the capacity of ecosystems to provide goods and services required by society, alter ecological processes, and can displace desirable species. They can reduce wildlife habitat quality, riparian area integrity, rangeland economic value, and enterprise net returns. The invasion process is regulated by characteristics of the invading plant and the community being invaded. The presence and spread of invasive plants is often symptomatic of underlying management problems that must be corrected before acceptable, long-term rangeland improvement can be achieved. Disturbance appears to be important early in the invasion process because it creates vacant niches that alien plants can …
Leafy Spurge (Euphorbia Esula) Genotype Affects Gall Midge (Spurgia Esulae) Establishment, Rodney G. Lym, Scott Nissen, Martha Rowe, Donald J. Lee, Robert A. Masters
Leafy Spurge (Euphorbia Esula) Genotype Affects Gall Midge (Spurgia Esulae) Establishment, Rodney G. Lym, Scott Nissen, Martha Rowe, Donald J. Lee, Robert A. Masters
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Greenhouse cage studies were conducted to determine the influence of shoot morphology and genetic variation on establishment of Spurgia sulae gall midge on seven leafy spurge genotypes. The genotypes were collected from South Dakota, North Dakota, Nebraska, Montana, Wyoming, Manitoba, and Austria. Genotypes from South Dakota and Nebraska were most susceptible to gall formation and had the highest larvae survival, while the genotypes from Montana and Manitoba were most resistant. Morphological characteristics of the leafy spurge stem tips, such as stem diameter leaf, length, width, and area did not correlate with gall formation or larvae survival. Chloroplast DNA restriction fragment …