Life Sciences Commons^™

Genetic Diversity Among Alfalfa Cultivars Using Ssr Markers, S. Flajoulot, J. Ronfort, P. Baudouin, T. Huguet, P. Barre, Christian Huyghe, B. Julier

IGC Proceedings (1997-2023)

Alfalfa (Medicago sativa) is an autotetraploid, allogamous and heterozygous species. Cultivated varieties are synthetic cultivars, usually obtained through 3 or 4 generations of panmictic reproduction of a set of various numbers of parents. The parents can be clones, half-sib or full-sib families. The breeders apply selection pressure for some agronomic traits, to induce changes in the genetic background. The objective of this study was to investigate the differentiation level among seven cultivars originating from one breeding program, and between these cultivars and the breeding pool, with eight SSR markers.

Application Of Molecular Markers In Genetic Resources Management Of Perennial Ryegrass, R. Van Treuren

IGC Proceedings (1997-2023)

Key points

1. Molecular markers are effective tools to support traditional approaches in plant genetic resources management.
2. Genetic diversity assessed for perennial ryegrass by AFLP analysis revealed differentiation of populations occurring in traditional Dutch grasslands from commercial varieties, but not from populations occurring in Dutch nature reserves.
3. No specific conservation measures were recommended to maintain the genetic diversity of perennial ryegrass occurring in traditional Dutch grasslands.
4. Pollination rates estimated by microsatellite analysis for a rejuvenated population of a perennial ryegrass genebank accession were very well described by an inverse quadratic function of inter-plant distance between potential mating pairs, while recorded flowering …

Development Of A Microsatellite Library In Lolium Perenne, J. King, I. P. King, D. Thorogood, L. Roberts, K. Skøt, K. Elborough

IGC Proceedings (1997-2023)

Lolium perenne, as one of the most important forage grasses of temperate regions, combines a number of very useful characteristics, e.g., good seedling establishment, with a low resistance to drought and limited winter hardiness. Trait selection and introgression can be greatly enhanced by the use of molecular markers in a genetic linkage map. The aim of this project was the generation of a genomic microsatellite library which when combined with microsatellites developed from a Genethresher database would give good genome coverage coupled to high levels of marker polymorphism.