Life Sciences Commons^™

Nebraska Promotes Sorghum And Pearl Millet Food Processing In East Africa, Intsormil

INTSORMIL Impacts and Bulletins

Sorghum and millet are ideal crops for many parts of Africa. However, sorghum and millet’s use as food sources for large urban populations in West Africa is hampered by 1) lack of infrastructure for conveying and demonstrating the food value of sorghum and millet to small business investors, 2) limitations on the supply of high quality grains to processors, 3) lack of small processors, 4) lack of educational resources related to small business development, 5) lack of product development expertise, and 6) lack of knowledge of evidence supporting nutritional claims associated with sorghum and millet consumption.

The Department of Food …

Enhanced Uv Inactivation Of Adenoviruses Under Polychromatic Uv Lamps, Karl G. Linden, Jeanette Thurston, Raymond Schaefer, James P. Malley Jr.

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Adenovirus is recognized as the most UV-resistant waterborne pathogen of concern to public health microbiologists. The U.S. EPA has stipulated that a UV fluence (dose) of 186 mJ cm^-2 is required for 4-log inactivation credit in water treatment. However, all adenovirus inactivation data to date published in the peer-reviewed literature have been based on UV disinfection experiments using UV irradiation at 253.7 nm produced from a conventional low-pressure UV source. The work reported here presents inactivation data for adenovirus based on polychromatic UV sources and details the significant enhancement in inactivation achieved using these polychromatic sources. When full-spectrum, medium-pressure …

Arkansas Corn And Grain Sorghum Performance Tests 2007, D. G. Dombek, R. D. Bond, I. L. Eldridge, R. M. Pryor

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas Division of Agriculture. The tests provide information to companies marketing seed within the state, and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

Arkansas Soybean Performance Tests 2007, D. G. Dombek, R. D. Bond, I. L. Eldridge, R. M. Pryor

Arkansas Agricultural Experiment Station Research Series

Soybean cultivar performance tests are conducted each year in Arkansas by the University of Arkansas Division of Agriculture. The tests provide information to companies developing cultivars and/or marketing seed within the State, and aid the Arkansas Cooperative Extension Service in formulating cultivar recommendations for soybean producers.

A Physiological Basis For Controlling Leafy Spurge On Nebraska Rangeland, Rob B. Mitchell, Corey Moffet, Ron Sosebee

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Applying herbicides at the appropriate physiological stage is an important consideration to effectively control leafy spurge.

Crop/Livestock Integration Effects On Soil Quality, Crop Production, And Soil Nitrogen Dynamics, Ellen B. Mallory

Electronic Theses and Dissertations

Regional integration of potato and dairy farms has developed in Maine through arrangements where manure, feed, and sometimes land, are exchanged between neighboring farms. The effects of integration on soil quality, crop production, nitrogen (N) cycling, and N loss were investigated in field and laboratory studies of contrasting amended (manure, compost, green manure, and supplemental fertilizer) and nonamended (fertilizer only) soil management systems within 2-year potato (Solanum tuberosum L.) rotations in the Maine Potato Cropping Systems Project (MPEP). Additionally, soil quality of 48 integrated and nonintegrated Maine potato and dairy farm fields was assessed. The MPEP’s amended soil system enhanced …

Livestock Production System Management Responses To Thermal Challenges, J. A. Nienabar, G. L. Hahn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The adaptive capabilities of animals and livestock production systems have been emphasized in this report. Biometeorology has a key role in rational management to meet the challenges of thermal environments. While the focus is primarily on cattle in warm or hot climates, the importance of dynamic animal responses to environmental challenges applies to all species and climates. Methods used to mitigate environmental challenges focus on heat loss/heat production balance. Under cold stress, reduction of heat loss is the key. Under heat stress, reduction of heat load or increasing heat loss are the primary management tools, although heat-tolerant animals are also …

Characterization Of Polymeric Proteins From Vitreous And Floury Sorghum Endosperm, B. Ioerger, S. R. Bean, M. R. Tuinstra, Jeffrey F. Pedersen, J. Erpelding, K. M. Lee, T. J. Herrman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Differences in protein content and composition between vitreous and floury endosperm were investigated using a number of different techniques. Differences in protein cross-linking between vitreous and floury endosperm were investigated using differential solubility, size exclusion chromatography (SEC), and analysis of sulfhydryl content and composition. Vitreous endosperm was found to have higher levels of total protein and kafirins, but floury endosperm had a higher proportion of γ-kafirins than the vitreous. Floury endosperm was found to have higher levels of SDS-soluble proteins than SDS-insoluble proteins extracted using sonication than vitreous endosperm. Conversely, vitreous endosperm had a greater proportion of the insoluble proteins. …

The Mycotoxin Threat To Food Safety, Ervin Balazs, James S. Schepers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This workshop entitled “Mycotoxins from the Field to the Table” held in Omaha, Nebraska, USA from 29 November to 1 December, 2006 evolved from the OECD (Organization for Economic Co-operation and Development) Co-operative Research Program on “Biological Resource Management” that was initiated in 1979. It currently focuses on three specific areas, namely the natural resources challenge, sustainability in practice, and the food chain. This workshop was organized under the sustainability and food chain themes.

The Cooperative Research Program's two major activities are promotion of expert exchanges by supporting postdoctoral fellowship awards up to 26 weeks in an OECD host laboratory …

Source Tracking Of Microbial Intrusion In Water Systems Using Artificial Neural Networks, Minyoung Kim, Christopher Y. Choi, Charles P. Garba

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A ‘‘what-if’’ scenario where biological agents are accidentally or deliberately introduced into a water system was generated, and artificial neural network (ANN) models were applied to identify the pathogenic release location to isolate the contaminated area and minimize its hazards. The spatiotemporal distribution of Escherichia coli 15597 along the water system was employed to locate pollutants by inversely interpreting transport patterns of Escherichia coli using ANNs. Results showed that dispersion patterns of Escherichia coli were positively correlated to pH, turbidity, and conductivity (R² = 0.90–0.96), and the ANN models successfully identified the source location of Escherichia coli introduced into …

Convergence Of Agriculture And Energy: Ii. Producing Cellulosic Biomass For Biofuels, Steven L. Fales, J. Richard Hess, Wallace Wilhelm, Don Erbach, William D. Provine, Kenneth P. Vogel, Todd A. Peterson, Edward C. A. Runge

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Global energy demand is increasing as known global petroleum supplies are decreas¬ing. Calls to supplement or replace the current fossil-based energy system with new, envi¬ronmentally and economically sustainable strategies continue to increase, especially in light of more expensive traditional energy sources. Various governmental agencies and working groups have set aggressive targets and timelines for decreasing fossil fuel consumption by substituting bio-based energy (Bush 2007; Foust et al. 2007; Perlack et al. 2005; Smith et al. 2004). The alignment and continuity of these goals is illustrated in Figure 1.
Current biofuel production in the United States relies primarily on corn grain …

Long-Term Tillage And Cropping Sequence Effects On Dryland Residue And Soil Carbon Fractions, Upendra M. Sainju, Thecan Caesar-Tonthat, Andrew W. Lenssen, Robert G. Evans, Robert Kolberg

Andrew W. Lenssen

Long-term management practices are needed to increase dryland C storage and improve soil quality. We evaluated the 21-yr effects of combinations of tillage and cropping sequences on dryland crop biomass (stems + leaves) returned to the soil, residue C, and soil C fractions at the 0- to 20-cm depth in a Dooley sandy loam (fine-loamy, mixed, frigid, Typic Argiborolls) in eastern Montana. Treatments were no-till continuous spring wheat (Triticum aestivum L.) (NTCW), spring-tilled continuous spring wheat (STCW), fall- and spring-tilled continuous spring wheat (FSTCW), fall- and spring-tilled spring wheat–barley (Hordeum vulgare L.) (1984–1999) followed by spring wheat–pea (Pisum sativum L.) …

Biofuel Feedstocks: The Risk Of Future Invasions, Joseph M. Ditomaso, Jacob N. Barney, Alison M. Fox

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In an effort to decrease greenhouse gas emissions, expand domestic energy production, and maintain economic growth, public and private investments are being used to pursue dedicated feedstock crops for biofuel production. Unlike food crops grown for grain-based ethanol (e.g., corn), which require high inputs of fertilizers and pesticides and typically are grown on prime agricultural land, proposed lignocellulose-based energy crops (e.g., switchgrass) typically have a neutral or negative carbon budget, require relatively few economic or environmental inputs, and can be cultivated on marginal, lower-productivity land. Thus, a rapidly growing industry related to crop selection, cultivar improvement, and conversion technologies is …

K12-Biotinylated Histone H4 Marks Heterochromatin In Human Lymphoblastoma Cells, Gabriela Camporeale, Anna M. Oommen, Jacob B. Griffin, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Covalent modifications of histones play crucial roles in chromatin structure and genomic stability. Recently, we reported a novel modification of histones: biotinylation of lysine residues. Here we provide evidence that K12-biotinylated histone H4 (K12Bio H4) maps specifically to both heterochromatin (alpha satellite repeats in pericentromeric regions) and transcriptionally repressed chromatin (γ-G globin and interleukin-2) in human lymphoblastoma cells. The abundance of K12Bio H4 in these regions was similar to that of K9-dimethylated histone H3, a known marker for heterochromatin. Likewise, K8-biotinylated histone H4 (K8Bio H4) mapped to heterochromatin, but the relative enrichment was smaller compared with K12Bio H4. Stimulation of …

Engineering, Nutrient Removal, And Feedstock Conversion Evaluations Of Four Corn Stover Harvest Scenarios, Reed L. Hoskinson, Douglas L. Karlen, Stuart J. Birrell, Corey W. Radtke, Wally Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Crop residue has been identified as a near-term source of biomass for renewable fuel, heat, power, chemicals and other bio-materials. A prototype one-pass harvest system was used to collect residue samples from a corn (Zea mays L.) field near Ames, IA. Four harvest scenarios (low cut, high-cut top, high-cut bottom, and normal cut) were evaluated and are expressed as collected stover harvest indices (CSHI). High-cut top and high-cut bottom samples were obtained from the same plot in separate operations. Chemical composition, dilute acid pretreatment response, ethanol conversion yield and efficiency, and thermochemical conversion for each scenario were determined. Mean …

Summaries Of Arkansas Cotton Research 2006, Derrick M. Oosterhuis

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Source Tracking Of Microbial Intrusion In Water Systems Using Artificial Neural Networks, Minyoung Kim, Christopher Y. Choi, Charles P. Gerba

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A ‘‘what-if’’ scenario where biological agents are accidentally or deliberately introduced into a water system was generated, and artificial neural network (ANN) models were applied to identify the pathogenic release location to isolate the contaminated area and minimize its hazards. The spatiotemporal distribution of Escherichia coli 15597 along the water system was employed to locate pollutants by inversely interpreting transport patterns of E. coli using ANNs. Results showed that dispersion patterns of E. coli were positively correlated to pH, turbidity, and conductivity (R² = 0.90–0.96), and the ANN models successfully identified the source location of E. coli introduced into …

Comparison Of Laboratory Methods And An In Situ Method For Estimating Nitrogen Mineralization In An Irrigated Silt-Loam Soil, Brian J. Wienhold

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen (N) mineralization makes a considerable contribution to crop-available N and is difficult to estimate. Reliable methods for measuring N mineralization are needed to produce data sets for developing N-mineralization models, as a component in fertilizer recommendation algorithms, and to assess the effect of management practices on N mineralization. Numerous methods are available for estimating N mineralization. Laboratory methods are relatively easy but may not reflect conditions in the field, and field methods are usually labor-intensive. A study was conducted to compare N-mineralization estimates using anaerobic and aerobic laboratory methods and an in situ field method for the 0- to …

The Effect Of Residue C:N Ratio On The Turnover Of N And C In Various Soil Organic Matter Fractions, Ana B. Wingeyer

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Identifying soil organic matter (SOM) fractions that contribute to soil indigenous nitrogen (N) supply and understanding their turnover under different management constitute necessary tools toward an efficient N use. The objectives of this study were: i) trace the endpoint of carbon (C) flux from residue inputs into SOM; and ii) asses the role of the light fraction (LF), mobile humic acid (MHA) fraction and calcium humate (CaHA) fraction as N sources for heterotrophic decomposition of fresh plant residues with contrasting C:N ratio. A long-term aerobic soil incubation was carried out on ¹⁵N-labeled soil samples from Lincoln and Mead, NE. …

Usda-Ars Lincoln Location Contact Information

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Grain, Forage, & Bioenergy Research Unit
Mid-West Livestock Research Unit
Soil & Water Conservation Research Unit
Employee Listing

The mission of the USDA / ARS programs at Lincoln, Nebraska is to improve the productivity, stability of production, sustainability and profitability of crop and livestock production systems in the Great Plains

The Lincoln Location is a part of the Northern Plains Area (NPA) of the Agricultural Research Service (ARS), a Research, Education and Economics (REE) agency, and the main in-house research arm of the United States Department of Agriculture (USDA)

Internode Structure And Cell Wall Composition In Maturing Tillers Of Switchgrass (Panicum Virgatum. L), Gautam Sarath, Lisa M. Baird, Kenneth P. Vogel, Robert B. Mitchell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This work examined cell composition gradients in maturing tillers of switchgrass (Panicum virgatuln L. ) with the aim of developing baseline information on this important forage and biomass crop. Flowering tillers were collected from plants raised from seeds in a greenhouse and field, harvested at soil level and separated into internodes beginning with the node subtending the peduncle. Internodes were analyzed using microscopy, by fiber digestion, high-performance liquid chromatography and by gas chromatography-mass spectrometry to obtain anatomical and compositional data. Microscopy demonstrated the development and maturation of cortical fibers which eventually became confluent with the fiber sheath surrounding vascular …

Cloning And Expression Of An Atrazine Inducible Cytochrome P450, Cyp4g33, From Chironomus Tentans (Diptera: Chironomidae), Diana K. Londono, Herbert A.A. Siqueira, Haichuan Wang, Gautam Sarath, Michael J. Lydy, Blair D. Siegfried

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Previous studies performed in our laboratory have measured the effect of atrazine exposure on cytochrome P450-dependent monooxygenase activity and have found increased activity in midge larvae (Chironomus tentans) as a result of atrazine exposure (1– 10 ppm). Here we report the cloning and expression of a specific C. tentans CYP4 gene that is responsive to atrazine induction with an open reading frame of 1678 bp which encodes a putative protein of 559 amino acid residues. Alignments of deduced amino acid sequences with other insect P450 genes and phylogenetic analysis indicated a high degree of similarity to other insect …

Comparative Genomics Reveals Functional Transcriptional Control Sequences In The Prop1 Gene, Robert D. Ward, Minchul Cho, S. A. Camper, Shannon W. Davis, Lori T. Raetzman, Timothy P.L. Smith, Constance Esposito, Robert H. Lyons, Jan-Fang Cheng, Edward M. Rubin, Simon J. Rhodes

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mutations in PROP1 are a common genetic cause of multiple pituitary hormone deficiency (MPHD). We used a comparative genomics approach to predict the transcriptional regulatory domains of Prop1 and tested them in cell culture and mice. A BAC transgene containing Prop1 completely rescues the Prop1 mutant phenotype, demonstrating that the regulatory elements necessary for proper Prop1 transcription are contained within the BAC. We generated DNA sequences from the PROP1 genes in lemur, pig, and five different primate species. Comparison of these with available human and mouse PROP1 sequences identified three putative regulatory sequences that are highly conserved. These are located …

Tillage And Rotation Effect On Corn–Soybean Energy Balances In Eastern Nebraska, G.-W. Rathke, Brian Wienhold, Wallace Wilhelm, W. Diepenbrock

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Data from a field experiment conducted in eastern Nebraska over 16 years (1986–2001) were used to determine the energy balance of corn (Zea mays L.) and soybean (Glycine max L.) as affected by tillage treatments and rotation. Tillage treatments included chisel plow, tandem disk, moldboard plow, ridge-tillage, no-till and subsoil tillage. Crop sequences were continuous corn, continuous soybean, corn in a corn–soybean rotation and soybean in a soybean–corn rotation. The energy balance was assessed by comparing the parameters: energy gain (net energy output), energy intensity (energy input per unit grain equivalent, GE) and output/input ratio. Changes in plant …

What We Do Know About Rice (Oryza Sativa) Hemoglobins, Veronica Lira-Ruan, Emily H. Ross, Mark S. Hargrove, Gautam Sarath, Raul Arredondo-Peter, Robert V. Klucas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nonsymbiotic hemoglobins (nsHb) are proteins widely distributed in land plants, however their function in plant organs is still not known. Rice nsHbs have been characterized in detail and represent a model to study plant nsHbs. Rice recombinant Hb1 (rHb1) has a very high affinity for O₂ (1,800 μM^-1 s^-1), because the dissociation constant is extremely low (k_off = 0.038 s^-1). X-ray analysis and site-directed mutagenesis showed that the low dissociation constant of rHb1 is partially due to the stabilization of bound O₂ by distal His. This characteristic suggests that the function of …

Arkansas Small-Grain Cultivar Performance Tests 2006-2007, J. T. Kelly, M. J. Emerson, R. K. Bacon, E. A. Milus

Arkansas Agricultural Experiment Station Research Series

Small-grain cultivar performance tests are conducted each year in Arkansas by the Arkansas Agricultural Experiment Station, Department of Crop, Soil, and Environmental Sciences. The tests provide information to companies developing cultivars and/or marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating cultivar recommendations for smallgrain producers.

B.R. Wells Rice Research Studies 2006, R. J. Norman, J. F. Meullenet, K. A.K. Moldenhauer

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

An Avidin-Based Assay For Histone Debiotinylase Activity In Human Cell Nuclei, Yap Ching Chew, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The covalent binding of biotin to histones participates in heterochromatin formation, cell cycle progression and cellular response to DNA breaks. Biotinylation of histones appears to be a reversible process, but the identity of enzymes that remove biotin marks is largely unknown. Our long-term goal is to identify histone debiotinylases in human cells. Here we developed an avidin-based plate assay to quantify histone debiotinylase activities in nuclear extracts. This assay is an essential first step in purifying and identifying histone debiotinylases from human cells. Using this assay, we demonstrated that debiotinylation of histones depends on temperature and pH, consistent with enzyme …

Diversified Cropping Systems In Semiarid Montana: Nitrogen Use During Drought, Andrew W. Lenssen, J. T. Waddell, G. D. Johnson, G. R. Carlson

Andrew W. Lenssen

Improved nitrogen use efficiency would be beneficial to agroecosystem sustainability in the northern Great Plains of the USA. The most common rotation in the northern Great Plains is fallow–spring wheat. Tillage during fallow periods controls weeds, which otherwise would use substantial amounts of water and available nitrogen, decreasing the efficiency of fallow. Chemical fallow and zero tillage systems improve soil water conservation, and may improve nitrogen availability to subsequent crops. We conducted a field trial from 1998 through 2003 comparing nitrogen uptake and nitrogen use efficiency of crops in nine rotations under two tillage systems, conventional and no-till. All rotations …

Modeling Beaver Dam Effects On Ecohydraulics And Sedimentation In An Agricultural Watershed, Mary Carla Mccullough, Dean E. Eisenhauer, Michael G. Dosskey, David M. Admiraal

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Populations of North American beaver (Castor canadensis) have increased in recent decades throughout the agricultural Midwestern U.S., leading to an increase in the frequency of beaver dams in small streams. The impact of beaver dams on channel structure in this region is not known. Our field observations indicate that beaver dams are too dynamic and their affects on channel structure occur over longer time frames than is practical to study with field measurements. Modeling is therefore needed to determine if beaver dams will help stabilize and aggrade incised streams. The objective of this paper is to determine how …