Life Sciences Commons^™

Sweet Corn Cultivar Evaluation For Northern Indiana, 2000, Elizabeth Maynard

Midwest Vegetable Trial Reports

Thirty-nine sugar-enhanced (se) sweet corn cultivars were evaluated at the Pinney-Purdue Ag Center, Wanatah, IN. Yield, ear size, and ear quality are reported.

Pumpkin Cultivar Performance In Northern Indiana, 2000, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

Pumpkin cultivars were evaluated in plots at County Line Orchard in Hobart, Indiana. Yield, fruit number, and average fruit weight for sixteen jack-o-lantern types, seven pie types, three mini pumpkins, and six specialty pumpkins are presented in this paper.

Sweet Corn Cultivar Evaluation For Northern Indiana, 2000, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

Thirty-nine sugar-enhanced (se) sweet corn cultivars were evaluated at the Pinney-Purdue Ag Center, Wanatah, IN. Yield, ear size, and ear quality are reported.

Pumpkin Cultivar Performance In Northern Indiana, 2000, Elizabeth Maynard

Midwest Vegetable Trial Reports

Pumpkin cultivars were evaluated in plots at County Line Orchard in Hobart, Indiana. Yield, fruit number, and average fruit weight for sixteen jack-o-lantern types, seven pie types, three mini pumpkins, and six specialty pumpkins are presented in this paper.

Herbicide Evaluation In Arkansas Cotton, 1999, Marilyn Mcclelland, Jim Barrentine, Ken Smith, Nilda Burgos

Arkansas Agricultural Experiment Station Research Series

Herbicidal weed control is economically important for production of cotton. Field experiments are conducted annually in Arkansas to evaluate the activity of developmental and commercial herbicides for selective control of weeds in cotton. These experiments serve both industry and Arkansas agriculture by providing information on the selectivity of herbicides still in the developmental stage and by comparing the activity of these new herbicides with that of recommended herbicides.

Two Large-Insert Soybean Genomic Libraries Constructed In A Binary Vector: Applications In Chromosome Walking And Genome Wide Physical Mapping, K. Meksem, K. Zobrist, E. Ruben, D. L. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

Large DNA insert libraries in binary T-DNA vectors can assist in the isolation of the gene(s) under-lying a quantitative trait locus (QTL). Binary vectors facilitate the transfer of large-insert DNA fragments containing a QTL from E. coli to Agrobacterium sp. and then to plants. We constructed two soybean large-insert libraries from cv. Forrest in the pCLD04541 (V41) binary vector after partial digestion of genomic high-molecular-weight DNA with BamHI or HindIII. The libraries contain 76,800 clones with an average insert size of 125 kb, and therefore represent 9.5-fold haploid genome equivalents. Colony hybridization using a chloroplast-specific probe infers that …

Cattle Lack Vascular Receptors For Escherichia Coli O157:H7 Shiga Toxins, Ingrid M. Pruimboom-Brees, Tim W. Morgan, Mark R. Ackermann, Evelyn A. Dean-Nystrom, James E. Samuel, Nancy A. Cornick, Harley W. Moon

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Escherichia coli O157:H7 causes Shiga toxin (Stx)-mediated vascular damage, resulting in hemorrhagic colitis and the hemolytic uremic syndrome in humans. These infections are often food-borne, and healthy carrier cattle are a major reservoir of E. coli O157:H7. We were interested in knowing why cattle are tolerant to infection with E. coli O157:H7. Cattle tissues were examined for the Stx receptor globotriaosylceramide (Gb₃), for receptivity to Stx binding in vitro, and for susceptibility to the enterotoxic effects of Stx in vivo. TLC was used to detect Gb3 in tissues from a newborn calf. Gb₃ was detected …

Murine Monoclonal Antibodies Specific For Lipopolysaccharide Of Escherichia Coli O26 And O111, Mildred Rivera-Betancourt, James E. Keen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Monoclonal antibody (MAb) 12F5 reacted with 35 Escherichia coli O26 isolates and cross-reacted with 1 of 365 non-E. coli O26 isolates. MAb 15C4 reacted with 30 E. coli O111 strains and 8 Salmonella O35 strains (possessing identical O antigen) but not with 362 other bacterial strains. Lipopolysaccharide immunoblots confirmed MAb O-antigen specificity.

B.R. Wells Rice Research Studies 1999, R. J. Norman, C. A. Beyrouty

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Before You Say Yes: A Planning Guide For Speakers, Charles A. Francis, Heidi Carter, Cris Carusi, James W. King

Department of Agronomy and Horticulture: Faculty Publications

We need guidelines to help us decide whether to accept invitations to speak, whether to a class on campus or a special interest group outside. As educators and workshop organizers, we could also use suggestions on how to approach potential speakers. This article describes a single-page format that can be used to guide the planning process. Essential elements include contact information, location and organization of the activity, audience, learning goals, expected content, conclusions, and evaluation. Use of this planning sheet can give organization to an often haphazard process of planning, and enhance the potential of achieving the learning goals of …

Comparison Of Three Leaf Area Index Meters In A Corn Canopy, Wally Wilhelm, K. Ruwe, Michael R. Schlemmer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Measurement of leaf area index (LAI) is critical to understanding many aspects of crop development, growth, and management. Avail- ability of portable meters to estimate LAI non-destructively has greatly increased our ability to determine this parameter during the cropping season. However, with several devices on the market, each with an independent set of protocols for gathering accurate estimates of LAI, it is necessary for scientists to have comparisons of these meters under field conditions before selecting one for purchase and use. The objective of our study was to compare the LAI estimates by three meters (AccuPAR, LAI-2000, and SunScan) to …

Simulating Inbred-Maize Yields With Ceres-Im, Daniel P. Rasse, Joe T. Ritchie, Wally Wilhelm, Jun Wei, Edward C. Martin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

CERES-Maize, which was designed for simulation of hybrid maize (Zea mays L.), cannot be applied directly to seed-producing inbred maize because of specific field operations and physiological traits of inbred maize plants. We developed CERES-IM, a modified version of CERES-Maize 3.0 that accommodates these inbred-specific operations and traits, using a set of phenological measurements conducted in Nebraska (NE), and further tested this model with a set of field data from Michigan (MI). Detasseling (i.e., removal of the tassels from the female plants) was conducted prior to silking. Male rows were removed approximately 10 d following 75% silking. The thermal …

Arkansas Agriculture Situation And Outlook 2000, Bruce Ahrendsen, Eric Wailes, Bruce Dixon, H. L. Goodwin Jr., Tony Windham

Arkansas Agricultural Experiment Station Research Series

Many farmers in Arkansas and other parts of the United States are experiencing financial stress. The purpose of this report is to highlight the situation of Arkansas farmers and to offer an outlook for 2000. The report emphasizes the production, price, income, financial, farmland value, and interest rate outlook for Arkansas farmers and considers the impact of the macroeconomy on agriculture. The contribution of poultry production to the Arkansas agricultural economy is also presented and analyzed.

Evaluation Of Stomoxys Calcitrans (Diptera: Muscidae) Behavioral Response To Human And Related Odors In A Triple Cage Olfactometer With Insect Traps, Raul A. Alzogaray, David A. Carlson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A triple cage olfactometer provided with insect traps was used for evaluating behavioral responses of Stomoxys calcitrans (L.) females to human skin and breath, CO₂, and L-lactic acid analogs. After demonstrating there were no significant differences caused by cage location or time of day, 3 sets of 3 olfactometer tests were performed in a day, every 2 h beginning at 0900 hours. When a human hand was used as attractant, the attraction (expressed as percentage of trapped flies) increased as a function of the time; an inverted U-shaped relationship between attractancy and air speed was observed; and variation …

Arkansas Cotton Variety Test 1999, Fred M. Bourland, N. R. Benson, J. M. Hornbeck, C. D. Capps Jr.

Arkansas Agricultural Experiment Station Research Series

The primary aim of the Arkansas Cotton Variety Test is to provide unbiased data regarding the agronomic performance of cotton varieties in the major cotton growing areas in Arkansas. This information helps seed dealers establish marketing strategies and assists producers in choosing varieties to plant. In this way the annual test facilitates the inclusion of new, improved genetic material into Arkansas cotton production. The 1999 test had 67 entries (including 25 transgenic genotypes and 35 first-year entries), which were evaluated at sixsites in eastern Arkansas. The presence of four transgenic and five first-year entries among the top 10 yielding entries …

Field Evaluation Of Herbicides On Small Fruit, Vegetable, And Ornamental Crops, 1999, Ron E. Talbert, Lance A. Schmidt, Mike L. Lovelance, Eric F. Scherder

Arkansas Agricultural Experiment Station Research Series

Growers generally use herbicides to efficiently produce high-quality fruit and vegetables for processing or fresh market sales. Because of the smaller acreage of these crops compared with major field crops, fewer herbicides are registered for use in fruit and vegetable crops than for field crops. Each year, new herbicides are evaluated under Arkansas growing conditions with the objective of improving the herbicide technology for the grower, processor, and ultimately the consumer. This report includes studies on the control of many of the more serious weed problems in important crops of this region, including snapbeans, spinach and other greens, southernpeas, tomatoes, …

Effects Of Acid Adaptation Of Escherichia Coli O157:H7 On Efficacy Of Acetic Acid Spray Washes To Decontaminate Beef Carcass Tissue, Elaine Berry, Catherine N. Cutter

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Exposure to low pH and organic acids in the bovine gastrointestinal tract may result in the induced acid resistance of E. coli O157:H7 and other pathogens that may subsequently contaminate beef carcasses. The effect of acid adaptation of E. coliO157:H7 on the ability of acetic acid spray washing to reduce populations of this organism on beef carcass tissue was examined. Stationary-phase acid resistance and the ability to induce acid tolerance were determined for a collection of E. coliO157:H7 strains by testing the survival of acid-adapted and unadapted cells in HCl-acidified tryptic soy broth (pH 2.5). ThreeE. coli …

Escherichia Coli O157:H7 Causes More-Severe Systemic Disease In Suckling Piglets Than In Colostrum-Deprived Neonatal Piglets, Evelyn A. Dean-Nystrom, Joachim F. L. Pohlenz, Harley W. Moon, Alison D. O’Brien

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Our objective was to determine if suckling neonatal piglets are susceptible to enterohemorrhagic Escherichia coli (EHEC) O157:H7 disease. Surprisingly, EHEC O157:H7 caused more-rapid and more-severe neurological disease in suckling neonates than in those fed an artificial diet. Shiga toxin-negative O157:H7 did not cause neurological disease but colonized and caused attaching-and-effacing intestinal lesions.

Intsormil 2000 Annual Report, John M. Yohe, Thomas Crawford, Jr., Joan Frederick, Dorothy Stoner

INTSORMIL Impacts and Bulletins

Presently, worldwide, more than 800 million people are hungry and over I billion are desperately poor, and food demand is increasing rapidly. The majority of poor live in rural areas in developing countries and agricultural and food systems development is vital to economic growth; improving environmental quality; strengthening nutrition, health and child survival; improving the status of women; and promoting democratization. It is estimated that by 2000, the number of people living in developing countries will grow from 4.9 billion to 6.8 billion people. More than 1.3 billion people today live on less than one dollar per day. It is …

Effect Of Vacuum On The Performance Of The Flame Ionization Detector Used For Vacuum-Outlet Gas Chromatography, Ulrich R. Bernier, Christopher L. Bray, Richard A. Yost

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Vacuum-outlet operation of short fused-silica open tubular columns for gas chromatography provides benefits to analysis speed by increasing the optimum velocity while minimizing the loss in resolution. Vacuum-outlet operation of a column with a gas chromatographic detector necessitates that the detector also be under vacuum. Simple modifications were made to a gas chromatograph-flame ionization detector (GC-FID) for vacuum-inlet and vacuum-outlet operation. The vacuum-inlet system was operated in the splitless mode to allow for efficient sample loading prior to operation at reduced pressures. The goal of FID operation at 100 torr or less was achieved by using oxygen in place of …

Physiology And Reproduction: Plasma 17Β-Estradiol Levels And Ovarian Interstitial Cell Structure In Embryonic Japanese Quail, M. A. Abdelnabi, M. R. Bakst, J. E. Woods, M. A. Ottinger

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Plasma concentrations of 17β-estradiol (E₂) and left ovarian histology were investigated by light and electron microscopy in female Japanese quail from Day 10 of embryonic development through Day 7, posthatch. Plasma E₂ levels remained relatively constant (102 to 140 pg/mL) in the embryo followed by a sharp decrease posthatch (47 to 70 pg/mL).

Beginning on Day 10 of incubation, cells in the medullary portion (medullary cell; MC) of the left ovaries exhibited ultrastructural evidence of steroidogenic capability. The MC had numerous lipid droplets in close proximity to the smooth endoplasmic reticulum (SER). Mitochondria were also …

Similarity And Divergence Among Viruses In The Genus Furovirus, Yukio Shirako, Nobuhiro Suzuki, Roy C. French

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nucleotide sequences of RNAs 1 and 2 of a Japanese strain of soil-borne wheat mosaic virus (SBWMV), the type species of the genus Furovirus, and sorghum chlorotic spot virus (SCSV) were determined from cloned cDNA. The relationship among the Japanese and US strains of SBWMV, SCSV, oat golden stripe virus (OGSV), and recently proposed Chinese wheat mosaic and European wheat mosaic viruses (CWMV and EWMV) were examined at the nucleotide and amino acid levels. Pairwise comparisons of genome-encoded proteins among the six viruses showed that the US strains of SBWMV and CWMV were the most closely related pair in RNA …

Yields And Nitrogen Dynamics In A Rice–Wheat System Using Green Manure And Inorganic Fertilizer, Milkha S. Aulakh, Tejinder S. Khera, John W. Doran, Kuldip Singh, Bijay Singh

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Integrating fertilizer N (FN) with legume green manures (GM) can foster sustainable and environmentally sound agricultural systems in subtropical, semiarid soils low in organic matter. A 4-yr replicated field experiment with irrigated rice (Oryza sativa L.) and wheat (Triticum aestivum L.) rotations was conducted on a sandy loam soil in the Punjab of India to evaluate the effects of incorporating cowpea (Vigna unguiculata L.) or sesbania (Sesbania aculeata L.) GM on crop productivity, N-use efficiency, and NO₃ leaching. Rice grain yields with pretransplant incorporation of 20 and 40 Mg GM ha^-1 (GM₂₀ and …

Oxygen Metabolism In Plantlbacteria Interactions: Characterization Of The Oxygen Uptake Response Of Plant Suspension Cells, C. Jacyn Baker, E. W. Orlandi, Kenneth L. Deahl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In recent years the accumulation of reactive oxygen species (ROS) has been studied in plant cell suspension systems treated with bacterial pathogens. However, the associated utilization of molecular oxygen has not been well characterized. Using a multi-electrode oxygen analyser, the rates of oxygen consumption by tobacco cells during bacterial interactions were monitored. Heat-killed (HK) bacteria, which initiate an immediate ROS response in plant cells, were used as an elicitor to avoid complications of oxygen consumption by viable bacteria. An increase in oxygen uptake by the tobacco cells occurred within 4 min after addition of HK-bacteria and lasted for about 10 …

Inhibition Of Cysteine And Aspartyl Proteinases In The Alfalfa Weevil Midgut With Biochemical And Plant-Derived Proteinase Inhibitors, Stephen E. Wilhite, Thomas C. Elden, Joze Brzin, Ann C. Smigocki

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Proteolytic activities in alfalfa weevil (Hypera postica) larval midguts have been characterized. Effects of pH, thiol activators, low-molecular weight inhibitors, and proteinase inhibitors (PIs) on general substrate hydrolysis by midgut extracts were determined. Hemoglobinolytic activity was highest in the acidic to mildly acidic pH range, but was maximal at pH 3.5. Addition of thiolactivators dithiothreitol (DTT), 2-mercaptoethanol (2-ME), or l-cysteine had little effect on hemoglobin hydrolysis at pH 3.5, but enhanced azocaseinolytic activity two to three-fold at pH 5.0. The broad cysteine PI E-64 reduced azocaseinolytic activity by 64% or 42% at pH 5 in the presence or …

Behavioral Response Of Stomoxys Calcitrans (Diptera: Muscidae) To Conspecific Feces And Feces Extracts, David Carlson, Raul Alzogaray, Jerome Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The attraction response of Stomoxys calcitrans (L.) to its own feces was evaluated in a triple cage olfactometer. Both time- and concentration-response relationships were obtained for female S. calcitrans exposed to cellulose sponges impregnated with fresh fly feces or filter papers treated with chloroform: methanol extracts of fresh fly feces in 6-min tests. Attraction to feces collected on cellulose sponges decreased as the air flow increased. Feces collected on cellulose sponges and held for 28-31 d retained attractive activity. More female flies were attracted than males to feces on sponges or to polar solvent extracts of feces-contaminated cages. The activity …

Design And Application Of A Polyclonal Peptide Antiserum For The Universal Detection Of Leptin Protein, Mark P. Richards, Thomas J. Caperna, Theodore H. Elasser, Christopher M. Ashwell, John P. Mcmurtry

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

An epitope-specific polyclonal antiserum was produced in rabbits immunized against a synthetic 15 amino acid peptide (QRVTGLDFIPGLHPV) derived from the coding sequence reported for the porcine leptin gene (GenBank Accession No. U59894). This peptide contains a core sequence comprised of eight amino acids (GLDFIPGL) that is totally conserved in all leptin proteins studied to date. Purified recombinant human, mouse, rat, pig, and chicken leptin proteins were separated by polyacrylamide gel electrophoresis (SDS-PAGE) and electro-blotted onto PVDF membranes. Western blots were developed employing the leptin-specific peptide antiserum with an alkaline-phosphatase-conjugated anti-rabbit IgG second antibody chromogenic system. The peptide antiserum was found …

Bovine Blastocyst-Derived Trophectoderm And Endoderm Cell Cultures: Interferon Tau And Transferrin Expression As Respective In Vitro Markers, Neil C. Talbot, Thomas J. Caperna, J. Lannett Edwards, Wes Garrett, Kevin D. Wells, Alan D. Ealy

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Continuous cultures of bovine trophectoderm (CT-1 and CT- 5) and bovine endoderm (CE-1 and CE-2) were initiated and maintained on STO feeder cells. CT-1 and CT-5 were derived from the culture of intact, 10- to 11-day in vitro-produced blastocysts. CE-1 and CE-2 were derived from the culture of immunodissected inner cell masses of 7- to 8-day in vitro-produced blastocysts. The cultures were routinely passaged by physical dissociation. Although morphologically distinct, the trophectoderm and endoderm both grew as cell sheets of polarized epithelium (dome formations) composed of approximately cuboidal cells. Both cell types, particularly the endoderm, grew on top …

Variation In Boll Weevil (Coleoptera: Curculionidae) Captures In Pheromone Traps Arising From Wind Speed Moderation By Brush Lines, Thomas W. Sappington, Dale W. Spurgeon

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Paired trap lines of Þve boll weevil pheromone traps each were placed on opposite sides of a brush line at six different sites on a plantation in the Lower Rio Grande Valley of Texas. Temperature, wind speed, and wind direction were monitored with a nearby weather station. We observed a strong negative relationship between mean daily wind speed and total daily capture of boll weevils. About half of the day-to-day variation in weevil captures was explained by wind speed alone. In addition, our data indicate that much of the variation between traps within days may arise from differences in local …

Evaporation Reduction Potential In An Undisturbed Soil Irrigated With Surface Drip And Sand Tube Irrigation, Masoud Meshkat, Richard C. Warner, Stephen R. Workman

Biosystems and Agricultural Engineering Faculty Publications

The efficiency of drip irrigation is highly dependent on evaporation losses occurring from the constantly saturated soil beneath emitters. Advent of subsurface drip irrigation is in part an approach to curb this inefficiency. An irrigation method, Sand Tube Irrigation (STI), is proposed to increase the efficiency of “Normal” surface applied drip Irrigation (NI method) on permanent tree crops without the need for burying the irrigation tubing. The sand tube consists of removing a soil core beneath the emitter and filling the void with coarse sand. A weighing lysimeter was constructed in the laboratory and instrumented to directly measure temporal evaporation …