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Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes Jan 2024

Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes

Electronic Theses and Dissertations

Managing weeds, improving soil health, and reducing the use of plastic mulch continue to be priorities for South Dakota vegetable farmers. Farmers have expressed an interest in integrating cover crops into their farm systems. Clover cover crops used as a living mulch within and along cash crop rows may aid in weed suppression, nitrogen fixation, and prevent soil erosion. However, prior research has shown challenges of incorporating living mulch due to yield decreases. Research conducted in 2022 and 2023 in eastern South Dakota investigated the effects of four clover and four in-row soil management treatments on broccoli and organic winter …


Deconstructing The Art Of Physical Weed Control, Jordan W. Parks May 2023

Deconstructing The Art Of Physical Weed Control, Jordan W. Parks

Electronic Theses and Dissertations

Farmers adjust physical weed control (PWC) tools to optimize efficacy based on observations of weeds, the crop, and soil conditions. These many variables make PWC research challenging. To study PWC tool settings more closely, we constructed a soil bin with a mobile tool carriage inside of a heated glasshouse. The soil bin was 2 m wide by 12 m long by 1 m high, with a tool carriage that can operate at 0.4 to 19.0 km h-1. Tool angle, spacing, depth, and speed can all be precisely adjusted from the tool carriage and control panel. The goal of …


Influence Of Biochar As A Soil Amendment On Soil Water Content And Wild Blueberry Physiology, Abigayl Novak May 2023

Influence Of Biochar As A Soil Amendment On Soil Water Content And Wild Blueberry Physiology, Abigayl Novak

Electronic Theses and Dissertations

Maine wild (or lowbush) blueberries (Vaccinium angustifolium Ait.) continue to face challenges imposed by climate change. Reduced frequency of precipitation and increased drought conditions have negatively impacted this crop since it resides in sandy soils with limited retention of water and nutrients. The wild blueberry plants growing in water- and nutrient-poor sandy soils are likely to have poor resilience to drought, resulting in a decline in berry yield during drought years. Thus, there is an urgent need to find a drought management solution for wild blueberries. Compared with other drought management practices, such as irrigation systems, mulching, and adopting drought-resistant …


Fusarium Head Blight Resistance Assessment In Barley, Tasneem Fathima Jan 2023

Fusarium Head Blight Resistance Assessment In Barley, Tasneem Fathima

Electronic Theses and Dissertations

Fusarium head blight (FHB), commonly called scab is the most devastating disease of wheat and barley caused by the ascomycete fungus, Fusarium graminearum. FHB affects both the quality and quantity of cereal grains affecting the supply chain of the food and feed industry and the marketability of the produce. Barley (Hordeum vulgare L.) is among the several crop species most vulnerable to FHB infection. An estimated 25% of Spring barley grain production in the United States is used to make malted beverages such as beer. Malting barley is a relatively high-value crop with high demands regarding grain quality. FHB directly …


Tracking Nitrogen Mineralization In The Presence Of Biochar Utilizing Complementary-Method Assays In East Central South Dakota, Andrew Calvin Engel Jan 2023

Tracking Nitrogen Mineralization In The Presence Of Biochar Utilizing Complementary-Method Assays In East Central South Dakota, Andrew Calvin Engel

Electronic Theses and Dissertations

Understanding soil N transformations in agricultural systems of the Northern Great Plains is crucial for guiding effective soil and nutrient management of this vital cropland. While the use of biochar in agriculture has attracted great attention recently, little has been reported on the seasonal dynamics of soil nitrogen (N) transformation and its response to biochar application. A field experiment was conducted over 2 seasons, spring (6/3/19-6/24/19) and summer (7/26/19-8/16/19), using four treatments or control soil, urea (224 kg N/ha), biochar (46,250 kg/ha), and urea+biochar (224 kg N/ha and 46,250 kg/ha, respectively). The results for both seasons showed biochar had no …


Enhancing Nitrogen Use Efficiency Through Ai-Powered Image Analysis And Innovative N-Rich Spot Method, Bobby Azad Jan 2023

Enhancing Nitrogen Use Efficiency Through Ai-Powered Image Analysis And Innovative N-Rich Spot Method, Bobby Azad

Electronic Theses and Dissertations

This study conducted in 2023 aimed to enhance nitrogen use efficiency (NUE) in wheat and corn grown in South Dakota. Based on dynamic weather conditions and other factor interactions, conventional nitrogen (N) recommendations need to be improved. Soil properties information, including electrical conductivity, was used to create management zones. In each zone, three N-rich spots were established as biosensors. Drones and satellites collected imagery data, and an AI-driven approach assessed the crop response to applied N. A dynamic N application approach, integrating aerial data with historical records, was developed and evaluated. Our methodology, at a 95% confidence level, resulted in …


Methods For Improving Potassium Fertilizer Recommendations For Corn In South Dakota, Andrew J. Ahlersmeyer Jan 2023

Methods For Improving Potassium Fertilizer Recommendations For Corn In South Dakota, Andrew J. Ahlersmeyer

Electronic Theses and Dissertations

Corn (Zea mays L.) is a vital commodity in South Dakota’s agricultural sector. Optimal corn production occurs when there are sufficient mineral nutrients in the soil, especially potassium (K). Applications of K fertilizer are used when soil test K (STK) levels are deficient. Therefore, producers need reliable, thoroughly tested fertilizer recommendations to make profitable decisions and maintain environmental stewardship. South Dakota K fertilizer recommendations have not been updated in nearly 20 years. Simultaneously, changes in corn genetics, management practices, and climate patterns suggest that the critical soil test value (CSTV) for STK may have shifted in that same time frame. …


Expanding The Use Of High-Performance Liquid Chromatography For Varietal Identification Or Verification Of Selected Cereal Crops, Johnna Jorgensen Jan 2023

Expanding The Use Of High-Performance Liquid Chromatography For Varietal Identification Or Verification Of Selected Cereal Crops, Johnna Jorgensen

Electronic Theses and Dissertations

High-performance liquid chromatography (HPLC) is a form of analytical chemistry where adsorption is a key factor in which the purpose is to separate, identify and quantify each component in a mixture. It is the optimal separation technique of chemical and biological compounds that are non-volatile including proteins and natural products (i.e., plant extracts). As each sample component interacts differently with the absorbent material within the column, the flow rates change leading to the separation of peaks, which are plotted via computer software. Most commonly HPLC analysis has been used for chemical manufacturing and pharmaceutical purposes. When using HPLC for crop …


Phosphorus Use And Management Based On Fertilizer Placement, Rate Of Application, And Soil Biota In No-Till Situations, Brennan Alexander Bingham Lewis Jan 2022

Phosphorus Use And Management Based On Fertilizer Placement, Rate Of Application, And Soil Biota In No-Till Situations, Brennan Alexander Bingham Lewis

Electronic Theses and Dissertations

Phosphorus (P) pollution has become a concern among multiple scientific organizations as it leads to eutrophication, an algal bloom that depletes lacustrine and marine ecosystems of native species. Multiple strategies can be implemented to reduce phosphorus loss from agriculture fields, which is often implicated as a cause of eutrophication. Soil phosphorus chemistry results in phosphate fertilizers absorbing to clay minerals over time. Soil phosphorus is lost from agricultural fields primarily through wind and water erosion. No-till practices prevent soil erosion, which reduces the phosphorus from loading into waterways. Fertilizer placement affects phosphorus loss. Surface application of phosphorus fertilizers increases the …


Effects Of Conservation Management Practices On Soil Health And Crop Yields In Eastern South Dakota, Alex Mclain Jan 2022

Effects Of Conservation Management Practices On Soil Health And Crop Yields In Eastern South Dakota, Alex Mclain

Electronic Theses and Dissertations

Conservation management practice adoption on agricultural land has increased in recent years due to increasing public and private investment. The anticipated impact of increasing conservation management practice adoption are reduced greenhouse gas emissions and improved soil, water, and air quality. Understanding how these conservation management practices affect agricultural producers economically is vital to properly incentivize these practices. The existing literature on how conservation management practices affect soil health and crop yields is mixed and generally has been conducted on experimental research stations. These studies may not fully account for the on-farm effects of conservation management practices. The objectives of this …


Study Of Root System Architectural Traits Of Oat And Response To Endophyte Inoculation And Drought Stress, Krishna Ghimire Jan 2022

Study Of Root System Architectural Traits Of Oat And Response To Endophyte Inoculation And Drought Stress, Krishna Ghimire

Electronic Theses and Dissertations

Oat is an important cereal crop grown worldwide. Oats have the potential to contribute to human health due to their unique nutritional attributes. Developing oat cultivars with efficient root systems able to extract heterogeneously distributed soil resources can help maintain yield under drought conditions and in nutrient poor soil. Various root traits determine the soil volume that is explored by the root system for resource acquisition. Knowledge about the genetic control of oat root traits and response to biotic and abiotic environmental factors is lacking. Identifying quantitative trait loci associated with root traits and understanding the response of roots to …


Effect Of Essential Oils In The Control Of Fungal Diseases In Small Grains, Esneider Mahecha Bojaca Jan 2022

Effect Of Essential Oils In The Control Of Fungal Diseases In Small Grains, Esneider Mahecha Bojaca

Electronic Theses and Dissertations

Spring wheat (Triticum aestivum L.) and oats (Avena sativa L.) are important crops due to their high consumption in our daily diet worldwide. They are the main food of consumption per capita due to the high amino-acid content as well of vitamins. However, grain production is limited due to the negative impact of the diseases that cause significant yield loss. Diseases in oats, such as crown rust caused by Puccinia coronata f. sp. avenae (Pca), and in wheat such as Fusarium head blight (FHB) caused by the fungus Fusarium graminearum. Foliar diseases such as tan spot incited by the fungi …


Soil Hydro-Physical Properties, Computed Tomography Measured Pore Parameters, And Soil Health Indicators As Influenced By Tillage And Crop Rotation Systems, Goutham Thotakuri Jan 2022

Soil Hydro-Physical Properties, Computed Tomography Measured Pore Parameters, And Soil Health Indicators As Influenced By Tillage And Crop Rotation Systems, Goutham Thotakuri

Electronic Theses and Dissertations

Long-term tillage and crop rotation systems are important agricultural management practices as these can have direct impact on the soil’s key properties. The objectives of this study were to (ⅰ) quantify the soil pore characteristics under long-term tillage and crop rotation using X-ray computed tomography (XCT) and to assess the relationships between XCT-measured pore parameters and soil hydro-physical properties; and (ⅱ) evaluate the impacts of long-term tillage and crop rotation on select soil health indicators. The objective (ⅰ) was carried out at Haskell Agricultural Laboratory (HAL), Concord, NE; and objective (ⅰi) was carried out at South Central Agricultural Laboratory (SCAL), …


Response Of Soil Biochemical And Physical Properties To Long Term Prairie Cordgrass And Kura Clover Intercropping System, Vaishnavi Varikuti Jan 2022

Response Of Soil Biochemical And Physical Properties To Long Term Prairie Cordgrass And Kura Clover Intercropping System, Vaishnavi Varikuti

Electronic Theses and Dissertations

Prairie cordgrass (Spartina pectinata) (PCG) is a warm-season perennial grass that can be used as a biofuel feedstock and can be grown on marginal lands. Previous studies on intercropping of a perennial legume i.e., kura clover (Trifolium ambiguum) (KC) with PCG can improve soil biochemical properties, increase biomass production, mitigate greenhouse gas (GHG) emissions while reducing the chemical fertilizer requirement. However, there is a lack of evidence about the effects of PCG production on soil biochemical and physical properties during the cropping season and at deeper soil depths in reference to support plant growth, environmental implications and enhance the soil …


Determining Effects Of Management Practices On Potato Early Dying And Soil Microbiome And Assessing Risk Of Fungicide Resistance In Verticillium Dahliae, Kedi Li Dec 2021

Determining Effects Of Management Practices On Potato Early Dying And Soil Microbiome And Assessing Risk Of Fungicide Resistance In Verticillium Dahliae, Kedi Li

Electronic Theses and Dissertations

Potato early dying (PED) is a yield-constraining soilborne disease of potato, caused by Verticillium spp. with V. dahliae being the predominant causal agent. Since the pathogen inhabits soil for long periods, PED management aims to reduce the population of V. dahliae in soil. Benzovindiflupyr and azoxystrobin are effective chemicals and frequently used in the control of V. dahliae. In this study, field trials were conducted at Aroostook Farm, Presque Isle, ME in 2019 and 2020. Chemical and biological products have been studied for PED control, and fungicide resistance was also examined. To evaluate fungicide resistance, benzovindiflupyr was characterized on …


Assessing Freezing Effect On Kiwifruit Cultivars And Mapping Suitable Areas For Growing The Crop In Eastern Texas, Lais De Oliveira Machado Dec 2021

Assessing Freezing Effect On Kiwifruit Cultivars And Mapping Suitable Areas For Growing The Crop In Eastern Texas, Lais De Oliveira Machado

Electronic Theses and Dissertations

Kiwifruit is a perennial vine originating from China where it has been grown for centuries. In the United States, green kiwifruit (Actinidia deliciosa) is primarily produced commercially in California. They are fuzzy, green fleshed and well known in the marketplace. Kiwifruit plants require low to moderate soil pH, adequate winter chilling and adequate precipitation to guarantee plant development and good fructification. Actinidia chinesis or golden kiwifruit are smooth skinned, feature golden flesh and are a more recent introduction into the global market. Kiwifruit crops have attributes that favor production in east Texas, including low pest problems, current long …


How Do Cover Crops Change Soil Health In A No-Till System?, Aysha Kirsten Tapp Ross Dec 2021

How Do Cover Crops Change Soil Health In A No-Till System?, Aysha Kirsten Tapp Ross

Electronic Theses and Dissertations

Nutrient levels and aggregation measurements are currently the most accurate means to measure soil health. It has been suggested that bacterial and fungal communities may prove to be a more accurate measure of soil health. In this study soil microbe communities and nutrient levels were compared in rye cover cropped soils to measure for differences between treatments. Effects between the microbial communities and environmental measurements were also measured within those treatments to test for correlations between soil health measures and microbial communities. The plots were put in a biennial corn (Zea mays L.) and soybean (Glycine max …


The Effects Of Gibberellic Acid, Smoke Water, And Cold Stratification On The Germination Of Native Perennial Seed, Zyreasha Tippins Aug 2021

The Effects Of Gibberellic Acid, Smoke Water, And Cold Stratification On The Germination Of Native Perennial Seed, Zyreasha Tippins

Electronic Theses and Dissertations

In this study, I evaluated the germination rates of twenty native plant species and their response to cold stratification as well as four chemical solution treatments—water (control), gibberellic acid, smoke water, and gibberellic acid with smoke water. Seeds were evaluated and counted twice a week. Of the twenty evaluated species, sixteen had germination rates over 3% or 1 seed out of 30. Stratification increased germination by 54% when compared to the control. For chemical treatments, gibberellic acid and gibberellic acid with smoke water were significantly different from the control and increased germination by an average of 47% and 48%, respectively. …


Characterization Of Dickeya Dianthicola And Pectobacterium Parmentieri Causing Blackleg And Soft Rot On Potato, Tongling Ge May 2021

Characterization Of Dickeya Dianthicola And Pectobacterium Parmentieri Causing Blackleg And Soft Rot On Potato, Tongling Ge

Electronic Theses and Dissertations

Potato blackleg and soft rot (PBSR), which can be caused by Dickeya spp. and Pectobacterium spp., is a serious problem worldwide. The recent outbreak of PBSR in the Northeastern USA, caused primarily by D. dianthicola, has resulted in significant economic losses since 2015. This seedborne disease is highly associated with and therefore spread by seed tuber distribution. To understand how the outbreak occurred and where the pathogen originated, a total of 1204 potato samples were collected from 11 northeastern states from 2015 to 2020. All the samples were processed for bacterial isolation and DNA extraction. Dickeya dianthicola and P. …


The Role Of The Auxin Biosynthesis And Signaling In Soybean Root Nodule Development, Mucahid Bozkus Jan 2021

The Role Of The Auxin Biosynthesis And Signaling In Soybean Root Nodule Development, Mucahid Bozkus

Electronic Theses and Dissertations

Nitrogen(N) is one of the most important plant nutrients for plant growth and yield, however, its abundance in the soil is not sufficient for profitable crop production. The use of chemical fertilizers helps address soil N deficiency in agriculture. However, due to the environmental pollution resulting from excessive use of fertilizers, alternative forms of N for agriculture are a necessity. Leguminous plants such as soybean (Glycine max) form a symbiotic association with N-fixing rhizobia to meet their N demands. Legume-rhizobia symbiosis results in the formation of unique structures called nodules where rhizobia convert atmospheric nitrogen into plant usable forms, thus …


Soil Organic Carbon, Hydro-Physical Properties, Pore Structure And Greenhouse Gas Fluxes Under Integrated Crop-Livestock System, Jashanjeet Kaur Dhaliwal Jan 2021

Soil Organic Carbon, Hydro-Physical Properties, Pore Structure And Greenhouse Gas Fluxes Under Integrated Crop-Livestock System, Jashanjeet Kaur Dhaliwal

Electronic Theses and Dissertations

Integrated crop-livestock system (ICLS) can improve soil organic carbon (SOC), enhance soil hydro-physical properties, and mitigate greenhouse gas (GHG) emissions. The objectives of this study were to: (i) investigate the influence of grazing, cover crop (CC), and a combination of grazing and CC on labile soil carbon (C) and nitrogen (N) fractions and soil enzyme activity, (ii) assess the impacts of short-term (3-4 years) and long-term (>30 years) ICLS on SOC, bulk density, structural quality and water infiltration, (iii) evaluate soil pore structural properties such as porosity, number of pores, pore size, pore shape, fractal dimension, anisotropy and tortuosity …


Cold Climate Grape Cultivar`S Physiological And Gene Expression Responses To Low And Freezing Temperatures, Turhan Yilmaz Jan 2021

Cold Climate Grape Cultivar`S Physiological And Gene Expression Responses To Low And Freezing Temperatures, Turhan Yilmaz

Electronic Theses and Dissertations

Grapevine (Vitis Vinifera), widely cultivated in the world and USA, is a significant and valuable fruit crop. After cold climate grapevine cultivars were released by breeding programs in the 1990s, the production of grapes expanded in the Northern cold climate region of the US. The objectives of this study were to test 1) freezing tolerance and chilling fulfillment, 2) the effect of pruning methods on yield and winter survival, and 3) transcriptomic changes in natural and controlled chilling conditions during chilling requirement fulfillment in cold climate grapevine cultivars. Pruning methods, spur (SP), short cane (SC), and spur plus short cane …


Influence Of Cover Crops On Water And Nutrient Cycles In South Dakota, Sam Ireland Jan 2021

Influence Of Cover Crops On Water And Nutrient Cycles In South Dakota, Sam Ireland

Electronic Theses and Dissertations

A major concern of farmers implementing cover crops into a crop rotation is a potential reduction in soil moisture and nutrients available for the following cash crop. If soil moisture or nutrients are limited, the following cash crop yield suffers. One option to conserve soil moisture is to terminate cover crops with herbicides prior to a killing freeze. The purpose of this study is to develop a better understanding of the impact of cover crops and termination timing to the water and nutrient cycles and furthermore, the agronomic impact to the following cash crop (corn). Field experiments were conducted in …


Evaluating The Current Weed Community In Wild Blueberry Fields And Ipm Strategies For Spreading Dogbane (Apocynum Androsaemifolium), Anthony G. Ayers Dec 2020

Evaluating The Current Weed Community In Wild Blueberry Fields And Ipm Strategies For Spreading Dogbane (Apocynum Androsaemifolium), Anthony G. Ayers

Electronic Theses and Dissertations

Lowbush blueberry (Vaccinium angustifolium) is Maine’s third largest crop (USDA 2020 a). From 2017 – 2019 the three seasons yield average was 27,200 tons were harvested from 19,500 acres for a value of $22,468,000 (USDA 2020 c). Lowbush blueberries are managed on a two-year cycle. Every other year, lowbush blueberry fields are pruned to the ground either through the use of a tractor mounted flail mower or a prescribed burn (Yarborough 2009). Pruning is a necessary part of managing lowbush blueberries as the second-year growth produces the highest yield but steadily declines in subsequent years (Drummond et al. …


Population Structure And Reproductive Biology Of Monilinia Vaccinii-Corymbosi (Reade) Honey In Lowbush Blueberry In Maine, Katherine A. Ashley May 2020

Population Structure And Reproductive Biology Of Monilinia Vaccinii-Corymbosi (Reade) Honey In Lowbush Blueberry In Maine, Katherine A. Ashley

Electronic Theses and Dissertations

Lowbush blueberry, Vaccinium angustifolium Aiton, is both an economically and culturally important crop in Maine, being one of the few endemic crops to North America. The fungus Monilinia vaccinii-corymbosi (Mvc) causes mummy berry disease and is a significant pathogen of both highbush (Vaccinium corymbosum) and lowbush blueberries. While impacts of this disease are not regularly documented, it is estimated that 30-50% of the yield in an unmanaged field can be lost because of Mvc. This disease is typically managed with fungicides or burning of the field during years when the field is pruned, however, the impacts to the …


Predicting Farinograph Stability Of Wheat Flour With Mixograph And Glutomatic Tests, Brooke Shumate Jan 2020

Predicting Farinograph Stability Of Wheat Flour With Mixograph And Glutomatic Tests, Brooke Shumate

Electronic Theses and Dissertations

Hard red spring wheat (Triticum aestivum L.; HRSW) flour is typically used to produce wheat-based foods where dough strength is a key quality component. Maintaining acceptable levels of dough strength is an important goal in the development of new HRSW cultivars. In a commercial setting, dough strength is often measured as Farinograph stability, though due to various resource constraints, stability is often predicted in breeding programs via other methods like the Mixograph. The objective of this research was to combine Glutomatic with Mixograph data to determine whether Farinograph stability predictions might be improved over the use of Mixograph data …


The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga Dec 2019

The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga

Electronic Theses and Dissertations

Dickeya dianthicola (Samson) causing blackleg and soft rot was first detected in potatoes grown in Maine in 2014. Previous work has suggested that insects, particularly aphids, may be able to vector bacteria in this genus between plants, but no conclusive work has been done to confirm this theory. In order to determine whether insect-mediated transmission is likely to occur in potato fields, two model potato pests common in Maine were used: the Colorado potato beetle (Leptinotarsa decimlineata Say) and the green peach aphids (Myzus persicae Sulzer). Olfactometry and recruitment experiments evaluated if either insect discriminates between infected and …


The Dispersal Pattern Of Thekopsora Minima In Wild Blueberry Determined By A Molecular Detection Method, Nghi Nguyen Aug 2019

The Dispersal Pattern Of Thekopsora Minima In Wild Blueberry Determined By A Molecular Detection Method, Nghi Nguyen

Electronic Theses and Dissertations

Blueberry rust caused by Thekopsora minima is a common disease in wild blueberry (Vaccinium angustifolium) and other Vaccinium genera. Understanding the spore dispersal pattern and disease cycle of fungal pathogens in wild blueberry is crucial for the development of a more efficient disease management program. Molecular assays for rapid detection and quantification of Thekopsora minima were developed to be incorporated with a spore trap sampling method and weather data collection to examine spore dispersal pattern and production in three different fields: Blueberry Hill Farm in Jonesboro, East Machias, and Spring Pond in Deblois, Maine, in three years 2014, …


Landscape Pattern And Wild Bee Communities In Maine, Brianne Du Clos May 2019

Landscape Pattern And Wild Bee Communities In Maine, Brianne Du Clos

Electronic Theses and Dissertations

Commercial production of lowbush blueberry (Vaccinium angustifolium Aiton) in Maine relies primarily on managed honeybee hives; however, naturally occurring wild bees are more efficient pollinators of the crop. Wild bees have short foraging distances and must nest near crop fields to provide pollination services. After crop bloom, the surrounding landscape must provide sufficient forage to maintain wild bee populations for the remainder of the growing season. Lowbush blueberries in Maine are produced in a mixed-use landscape with two distinct landscape contexts. Here, we document bee communities and habitat resources (nesting and floral) in power line rights-of-way and eight land …


Nodule Zone-Specific Gene Expression In Soybean, Sadikshya Aryal Jan 2019

Nodule Zone-Specific Gene Expression In Soybean, Sadikshya Aryal

Electronic Theses and Dissertations

Nitrogen is one of the most limiting nutrients for plant growth and yield. Leguminous plants such as soybean (Glycine max) have developed the ability to form a symbiotic association with nitrogen fixing rhizobia. This symbiotic association results in the formation of unique structures called nodules that originate from root cortex via de novo cell differentiation. During soybean nodule development, two major nodule zones, the Nodule primordium (Npr) in the center and the nodule parenchyma (Npa) in the periphery, are clearly distinguishable. Npr gives rise to infection zone (IZ), and the Npa holds vascular bundles. However, it is not clear what …