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Full-Text Articles in Engineering
Classification And Biological Identity Of Complex Nano Shapes, Luca Boselli, Hender Lopez, Wei Zhang, Qi Cai, Valeria A. Giannone, Jingji Li, Alirio Moura, João M. De Araujo, Jennifer Cookman, Valentina Castagnola, Yan Yan, Kenneth A. Dawson
Classification And Biological Identity Of Complex Nano Shapes, Luca Boselli, Hender Lopez, Wei Zhang, Qi Cai, Valeria A. Giannone, Jingji Li, Alirio Moura, João M. De Araujo, Jennifer Cookman, Valentina Castagnola, Yan Yan, Kenneth A. Dawson
Articles
Everywhere in our surroundings we increasingly come in contact with nanostructures that have distinctive complex shape features on a scale comparable to the particle itself. Such shape ensembles can be made by modern nano-synthetic methods and many industrial processes. With the ever growing universe of nanoscale shapes, names such as “nanoflowers” and “nanostars” no longer precisely describe or characterise the distinct nature of the particles. Here we capture and digitise particle shape information on the relevant size scale and create a condensed representation in which the essential shape features can be captured, recognized and correlated. We find the natural emergence …
Intracellular Localisation, Geno- And Cytotoxic Response Of Poly N-Isopropylacrylamide (Pnipam) Nanoparticles To Human Keratinocyte (Hacat) And Colon Cells (Sw 480), Pratap Naha, Kunal Bhattacharya, Tiziana Tenuta, Kenneth Dawson, Iseult Lynch, Amaya Gracia, Fiona Lyng, Hugh Byrne
Intracellular Localisation, Geno- And Cytotoxic Response Of Poly N-Isopropylacrylamide (Pnipam) Nanoparticles To Human Keratinocyte (Hacat) And Colon Cells (Sw 480), Pratap Naha, Kunal Bhattacharya, Tiziana Tenuta, Kenneth Dawson, Iseult Lynch, Amaya Gracia, Fiona Lyng, Hugh Byrne
Articles
PNIPAM nanoparticles, with and without a covalentlylinked fluorescent label, were prepared by a free radical polymerisation technique. The cyto and genotoxicity of PNIPAM nanoparticles were analysed in two representative mammalian cell lines, SW480, a colon, and HaCaT, a dermal cell line. Physical characterisation in terms of particle size and zeta potential of the PNIPAM nanoparticles was carried out both in aqueous solution and in the appropriate cell culture media. Uptake and colocalisation of fluorescently labelled PNIPAM nanoparticles was monitored in both cell lines using confocal laser scanning microscopy. Genotoxicity analysis using the Comet assay was performed in both cell lines …