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Full-Text Articles in Engineering

Use Of 16s Rrna Gene Terminal Restriction Fragment Analysis To Assess The Impact Of Solids Retention Time On The Bacterial Diversity Of Activated Sludge, Pascal E. Saikaly, Peter George Stroot, Daniel B. Oerther Oct 2005

Use Of 16s Rrna Gene Terminal Restriction Fragment Analysis To Assess The Impact Of Solids Retention Time On The Bacterial Diversity Of Activated Sludge, Pascal E. Saikaly, Peter George Stroot, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes was used to investigate the reproducibility and stability in the bacterial community structure of laboratory-scale sequencing batch bioreactors (SBR) and to assess the impact of solids retention time (SRT) on bacterial diversity. Two experiments were performed. In each experiment two sets of replicate SBRs were operated for a periods of three times the SRT. One set was operated at an SRT of 2 days and another set was operated at an SRT of 8 days. Samples for T-RFLP analysis were collected from the two sets of replicate reactors. HhaI, …


Biofilm Removal Using Bubbles And Sound, Michael R. Parini Jul 2005

Biofilm Removal Using Bubbles And Sound, Michael R. Parini

Theses and Dissertations

Bacteria in biofilms adhere well to surfaces and are quite difficult to remove. Oral plaque is one example of a biofilm. Many researchers have studied ways to remove plaque and bacteria from surfaces. It has been found that the passage of a bubble across a surface to which bacteria has adhered can remove the bacteria from the surface. Biofilms of Streptococcus mutans were grown on glass coverslips as a simple model for oral plaque. The coverslips were mounted in a Plexiglas chamber filled with artificial saliva. A bubble stream was directed at the mounted biofilm. The velocity, gas fraction, median …