Engineering Commons^™

Isolation And Characterization Of Bacteria In A Toluene-Producing Enrichment Culture Derived From Contaminated Groundwater At A Louisiana Superfund Site, Madison Mikes

LSU Master's Theses

In an effort to better understand the role that various microbes may play in toluene production, bacteria from a toluene-producing enrichment culture derived from contaminated groundwater at a Superfund site were cultivated and isolated on low nutrient solid media. A total of 14 solid medium formulations containing varying pH ranges, carbon sources, solidifying agents, and incubation gas headspaces were used to obtain 278 isolates in pure culture. Isolated bacteria, identified using partial 16S rRNA gene sequences, were most closely related with the genera Anoxybacillus, Azospira, Bacillus, Bradyrhizobium, Cellulosimicrobium, Micrococcus, and Propionicimonas.

Further attempts …

Factors Controlling Macondo Oil Biodegraqdation On A Rapidly Eroding Coastal Headlands Beach, Marilany G. Urbano

LSU Master's Theses

Fourchon Beach, located along the southeast Louisiana coast, was affected by the oil leaking from the British Petroleum’s Deepwater Horizon Oil rig explosion in 2010. The oil, labeled MC252 oil, came ashore in a stable emulsion form, contaminating the sand, salt pan, marsh and mangroves along the beach. A unique oil form, labeled SSRB (small surface residue ball) was formed from the MC252 oil and a combination of environmental factors. The SSRB is an aggregate of sand and emulsified oil, and forms a large portion of the oil still present on the supratidal zone of Fourchon Beach. SSRBs have not …