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Articles 1 - 12 of 12
Full-Text Articles in Engineering
Optimal Conditions Of Fermentation Of The Pichia Pastoris Yeast Expressing The Recombinant Pres2-S Protein (M-Hbsag) Of Hepatitis B Virus (Hbv), Sasmakоv Sobirdjan, Ashirоv Оybek, Abdurakhmanоv Jaloliddin, Xasanоv Shuhrat
Optimal Conditions Of Fermentation Of The Pichia Pastoris Yeast Expressing The Recombinant Pres2-S Protein (M-Hbsag) Of Hepatitis B Virus (Hbv), Sasmakоv Sobirdjan, Ashirоv Оybek, Abdurakhmanоv Jaloliddin, Xasanоv Shuhrat
CHEMISTRY AND CHEMICAL ENGINEERING
The aim of this study is determination of optimal fermentation conditions of the Pichia pastoris yeast expressing the recombinant Pres2-S protein of hepatitis B virus (HBV). For this purpose we investigated the main factors influencing for the cultivation of recombinant strain of the yeast Pichia pastoris pPIC3,5-S-HBsAg - the composition of the nutrient medium, pH and dissolved oxygen (DO). As a result, by enzyme-linked immunosorbent assay (ELISA) the optimal concentration of methanol ≈1.0 %, which is the initiator and the only source of carbon for protein expression in the Mut+ phenotype Pichia pastoris yeast cells was determined. In the selected …
Physical And Chemical Characteristics Of Supramolecular Complexes Of Glycirrizinic Acid With Phytohormones, Djuraev Tulqin, Esanov Rakhmatillo, Gafurov Makhmudjon, Kushiev Khabibjon
Physical And Chemical Characteristics Of Supramolecular Complexes Of Glycirrizinic Acid With Phytohormones, Djuraev Tulqin, Esanov Rakhmatillo, Gafurov Makhmudjon, Kushiev Khabibjon
CHEMISTRY AND CHEMICAL ENGINEERING
The process of formation of supramolecular complexes of glycyrrhizinic acid with some phytohormones (indolylacetic acid, indolylbutyric acid, α-naphthyl acetic acid and kinetin) were studied with the aim of creation the plant growth stimulator. The complexes structure was studied using IR and UV spectroscopy methods. The prepared compounds described by some physicochemical parameters. The stability constants and stoichiometric composition of complexes were determined in an aqueous solution at pH of 7,2 using UV spectrometry method. In result, all complexes have composition as 1:1 ratio, except the kinetin complex which has 2:1 ratio. The stability constants were equal: KGA:IAA=1,86±1×104; KGA:IBA=2,51±1×103; KGA:NAA =2,70±1×103 …
Study Of The Process For The Removal Of Dyeing Matters Of The Plant Occimum Basilicum L. Lamiaceae, Usmonjonova Khulkar, Atkhamova Saida, Dodaev Kuchkor
Study Of The Process For The Removal Of Dyeing Matters Of The Plant Occimum Basilicum L. Lamiaceae, Usmonjonova Khulkar, Atkhamova Saida, Dodaev Kuchkor
CHEMISTRY AND CHEMICAL ENGINEERING
The aim of the work is dedicated isolation of anthocyanins by ethanol from herbal plant of Occimum basilicum L. Lamiaceae basis. The were studied influencing of temperature, concentration of ethanol to isolation of anthocyanins. There were passed investigations on studying influence level of heating and hydromodule system of rav material-solvent to maximal isolating of anthocyanins. The colors define by fotokolorometrical method at wave length 400-600 nm. There are shown at 540 nm maximal peak of anthocyanins and it is geven as a grapfical diagram. From the obtained depends on content of alcohol in aquatic solution from outlet of colors is …
Study Of Recombinant Protein Fractions From The Bombyx Mori And Pichia Pastoris Using "Native Page" Method, Abdurhakhmanov Jaloliddin, Sasmakov Sobirdjan, Khasanov Shuhrat, Ashirov Оybek
Study Of Recombinant Protein Fractions From The Bombyx Mori And Pichia Pastoris Using "Native Page" Method, Abdurhakhmanov Jaloliddin, Sasmakov Sobirdjan, Khasanov Shuhrat, Ashirov Оybek
CHEMISTRY AND CHEMICAL ENGINEERING
We have studied the recombinant protein fractions of ̴ 24-34 kDa obtained from Bombyx mori - silkworm larvae and yeast Pichia pastoris. Fractions, containing the recombinant proteins by gel-electrophoresis using the “Native PAGE” method, which allows for differentiation of proteins according to their natural (surface) charge and conformational properties were studied. The results showed that the recombinant protein fraction from Bombyx mori (̴ 34 kDa) consisted of four components and fraction from Pichia pastoris (̴ 24 kDa) consisted one component. The most optimal conditions for the “Native PAGE”- the main gel: Tris HCl (pH-8.8) 244 mM, Bis-Acrylamide 10%, TEMED 0.08%, …
Physical And Chemical Characteristics Of Supramolecular Complexes Of Glycirrizinic Acid With Phytohormones, Djuraev Tulqin, Esanov Rakhmatillo, Gafurov Makhmudjon, Kushiev Khabibjon
Physical And Chemical Characteristics Of Supramolecular Complexes Of Glycirrizinic Acid With Phytohormones, Djuraev Tulqin, Esanov Rakhmatillo, Gafurov Makhmudjon, Kushiev Khabibjon
CHEMISTRY AND CHEMICAL ENGINEERING
The process of formation of supramolecular complexes of glycyrrhizinic acid with some phytohormones (indolylacetic acid, indolylbutyric acid, α-naphthyl acetic acid and kinetin) were studied with the aim of creation the plant growth stimulator. The complexes structure was studied using IR and UV spectroscopy methods. The prepared compounds described by some physicochemical parameters. The stability constants and stoichiometric composition of complexes were determined in an aqueous solution at pH of 7,2 using UV spectrometry method. In result, all complexes have composition as 1:1 ratio, except the kinetin complex which has 2:1 ratio. The stability constants were equal: KGA:IAA=1,86±1×104; KGA:IBA=2,51±1×103; KGA:NAA =2,70±1×103 …
Study Of The Process For The Removal Of Dyeing Matters Of The Plant Occimum Basilicum L. Lamiaceae, Usmonjonova Khulkar, Atkhamova Saida, Dodaev Kuchkor
Study Of The Process For The Removal Of Dyeing Matters Of The Plant Occimum Basilicum L. Lamiaceae, Usmonjonova Khulkar, Atkhamova Saida, Dodaev Kuchkor
CHEMISTRY AND CHEMICAL ENGINEERING
The aim of the work is dedicated isolation of anthocyanins by ethanol from herbal plant of Occimum basilicum L. Lamiaceae basis. The were studied influencing of temperature, concentration of ethanol to isolation of anthocyanins. There were passed investigations on studying influence level of heating and hydromodule system of rav material-solvent to maximal isolating of anthocyanins. The colors define by fotokolorometrical method at wave length 400-600 nm. There are shown at 540 nm maximal peak of anthocyanins and it is geven as a grapfical diagram. From the obtained depends on content of alcohol in aquatic solution from outlet of colors is …
Study Of Recombinant Protein Fractions From The Bombyx Mori And Pichia Pastoris Using "Native Page" Method, Abdurhakhmanov Aloliddin, Sasmakov Sobirdjan, Khasanov Shuhrat, Ashirov Оybek
Study Of Recombinant Protein Fractions From The Bombyx Mori And Pichia Pastoris Using "Native Page" Method, Abdurhakhmanov Aloliddin, Sasmakov Sobirdjan, Khasanov Shuhrat, Ashirov Оybek
CHEMISTRY AND CHEMICAL ENGINEERING
We have studied the recombinant protein fractions of ̴ 24-34 kDa obtained from Bombyx mori - silkworm larvae and yeast Pichia pastoris. Fractions, containing the recombinant proteins by gel-electrophoresis using the “Native PAGE” method, which allows for differentiation of proteins according to their natural (surface) charge and conformational properties were studied. The results showed that the recombinant protein fraction from Bombyx mori (̴ 34 kDa) consisted of four components and fraction from Pichia pastoris (̴ 24 kDa) consisted one component. The most optimal conditions for the “Native PAGE”- the main gel: Tris HCl (pH-8.8) 244 mM, Bis-Acrylamide 10%, TEMED 0.08%, …
Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek
Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek
CHEMISTRY AND CHEMICAL ENGINEERING
The article discusses use of the insect cell cultures for biotechnological purposes. Optimal conditions for the cultiva-tion of Spodoptera exigua and Lymantria dispar cell lines were selected.
Development Of A Method Of Oriented Immobilization Of Lipases On A Solid Carrier, Ziyavitdinov Jamolitdin Fazlitdinovich, Inogamov Utkir Kudratillayevich, Berdiev Nodir Sharifovich, Ishimov Uchkun Jomuradovich
Development Of A Method Of Oriented Immobilization Of Lipases On A Solid Carrier, Ziyavitdinov Jamolitdin Fazlitdinovich, Inogamov Utkir Kudratillayevich, Berdiev Nodir Sharifovich, Ishimov Uchkun Jomuradovich
CHEMISTRY AND CHEMICAL ENGINEERING
The methods of oriented immobilization of lipases on a solid carrier - Silochrome C-80 have been developed. The resulting sorbents were tested in the process of obtaining biodiesel from cotton oil. It was shown that immobilized lipase on Silochrome C-80 with NH2 group has the highest transetrification ability and stability, while in sorbents with the enzyme immobilized with -COOH and -SH groups this value was 87 and 53%, respectively. Biocatalysis using an affinity sorbent of immobilized lipase with the –NH2 group has a high operational stability, which, after 240 hours of operation, retains more than 50%, and after 480 hours, …
Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek
Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek
CHEMISTRY AND CHEMICAL ENGINEERING
The article discusses use of the insect cell cultures for biotechnological purposes. Optimal conditions for the cultiva-tion of Spodoptera exigua and Lymantria dispar cell lines were selected.
Development Of A Method Of Oriented Immobilization Of Lipases On A Solid Carrier, Ziyavitdinov Jamolitdin Fazlitdinovich, Inogamov Utkir Kudratillayevich, Berdiev Nodir Sharifovich, Ishimov Uchkun Jomuradovich
Development Of A Method Of Oriented Immobilization Of Lipases On A Solid Carrier, Ziyavitdinov Jamolitdin Fazlitdinovich, Inogamov Utkir Kudratillayevich, Berdiev Nodir Sharifovich, Ishimov Uchkun Jomuradovich
CHEMISTRY AND CHEMICAL ENGINEERING
The methods of oriented immobilization of lipases on a solid carrier - Silochrome C-80 have been developed. The resulting sorbents were tested in the process of obtaining biodiesel from cotton oil. It was shown that immobilized lipase on Silochrome C-80 with NH2 group has the highest transetrification ability and stability, while in sorbents with the enzyme immobilized with -COOH and -SH groups this value was 87 and 53%, respectively. Biocatalysis using an affinity sorbent of immobilized lipase with the –NH2 group has a high operational stability, which, after 240 hours of operation, retains more than 50%, and after 480 hours, …
Obtaining Of The “Green Flourecent Protein - Gfp” In Yeast Of Pichia Pastoris As Repoter Of Heterological Protein Expression, Ashirоv Оybek, Sasmakоv Sobirdjan, Abdurakhmanоv Jaloliddin, Hasanоv Shuhrat
Obtaining Of The “Green Flourecent Protein - Gfp” In Yeast Of Pichia Pastoris As Repoter Of Heterological Protein Expression, Ashirоv Оybek, Sasmakоv Sobirdjan, Abdurakhmanоv Jaloliddin, Hasanоv Shuhrat
CHEMISTRY AND CHEMICAL ENGINEERING
Based on pPIC3.5 and pPIC9 yeast vectors the recombinant plasmid DNA pPIC3.5-GFP (8464 bp) and pPIC9-GFP (8718 bp) containing cDNA (719 bp) of Green fluorescent protein (GFP) were cloned. The obtained plasmids were transformed into yeast cells of the GS115 Pichia pastoris strain. Transformants are selected on histidine-deficient medium. Recombinant clones of pPIC3.5-GFP and pPIC9-GFP were identified by detecting cell fluorescence. GFP expression in yeast is confirmed by green fluorescence. Using electrophoresis in PAAG the molecular weight of the synthesized protein (≈27 kDa) is determined. The resulting reporter constructs and recombinant strains can be used to study the expression level …