Engineering Commons^™

Early Detection Of Asthma Via Enhanced Resolution Of Small Airways Using Ultra-Low Frequency Impulse Oscillometry, Christopher M. Aguilar

Open Access Theses & Dissertations

Examination of lung function for diagnosis and monitoring of diseases such as chronic obstructive pulmonary disease (COPD) and asthma can be performed with a variety of pulmonary function testing (PFT) techniques. The most commonly prescribed of these procedures are spirometry and whole-body plethysmography. However, these active participation tests depend on forced breathing maneuvers, may be physically exhausting, might not produce enough information, and are susceptible to being subject-dependent. In contrast, passive PFT approaches such as the forced oscillation technique (FOT) and the impulse oscillometry system (IOS) are increasingly being utilized for measuring lung mechanics because they require only minimal participation, …

Formulation Strategies To Enhance Solubility And Permeability Of Small Molecules For Drug Delivery Applications, Pradeep Kumar Bolla

Open Access Theses & Dissertations

All the new chemical entities/drug molecules intended for therapeutic use must be administered using an appropriate delivery system/dosage form to achieve maximum bioavailability. However, designing a drug delivery system is complex as several factors such as lipophilicity, molecular mass, crystallinity, ionic charge, polymorphic forms and hydrogen bonding) affect the solubility and permeability of these molecules. Biopharmaceutics drug classification system (BCS) categorizes the existing drugs into four classes based on the aqueous solubility and membrane permeability and it is reported that >70% of the drugs are poorly soluble and belong to BCS class II and BCS class IV. Several physical, chemical …

Supercritical Processed Decellularized Extracellualr Matrix As Regeneration Therapeutics Applying 3d Printing, Seungwon Chung

Open Access Theses & Dissertations

Extracellular matrix has been broadly applied and show great promise in medical applications and tissue engineering. Product of extracellular matrix (ECM) should be treated with decellularization and purification process. Supercritical carbon dioxide treatment is of particular interest for purifying ECM because of its medically available and rapid speed of process. However, it is not fully researched in treatment of biomaterials for tissue engineering. Therefore, we investigated optimal conditions of supercritical carbon dioxide processing in different extracting parameters from porcine adipose tissue. The 3-day, dual treatment including enzymatic decellularization and supercritical fluid extraction of pork adipose tissue were performed. Two types …

3d Printed Alginate-Based Zinc Oxide Nanoparticle Scaffolds For Wound Healing, Carol Cleetus

Open Access Theses & Dissertations

Effective treatment of chronic wounds is essential for the prevention of infection and scar tissue formation, requiring a novel approach to address the limitations of existing options. This study utilizes Calcium Chloride (CaCl2) crosslinked alginate, a common biomaterial widely used in wound dressing, and UV activated Zinc Oxide (ZnO) nanoparticles (NPs) for their potential hydroxyl radical mediated antibacterial applications. ZnO NPs were synthesized by combustion method from Zinc Nitrate Hexahydrate and Sucrose. Scanning Electron Microscopy (SEM) and X-Ray Diffraction (XRD) characterization were used to verify composition and NP size. Terephthalic Acid, used as a fluorescent probe, confirmed the generation of …

Regulation Of Schlafen 11 Expression, Christian Waldemar Corona Ayala

Open Access Theses & Dissertations

Schlafen 11 (SLFN11) is a member a family of proteins that can be found in mammals. Members of this family are involved in important functions. SLFN11 in particular has been identified as a restriction factor for several evolutionarily disparate viruses such as HIV-1 and Flaviviruses and to play an important role in DNA repair, sensitizing cells to chemotherapy. Despite its relevance SLFN11 remains largely uncharacterized. The aim of this research was to achieve a more comprehensive understanding the regulation mechanism of SLFN 11 expression. Before our research, it was only known that type I interferon (IFN) regulates SLFN11 levels. However, …

To Or Through The Skin: A Rational Design Of Transdermal Drug Delivery, Isaac Giovanni Deaguero

Open Access Theses & Dissertations

The skin is the largest organ in the body and plays many crucial roles through diverse functions ranging from protection, temperature regulation, energy storage via fat deposits, and is used to communicate with our surroundings. Various methods have been attempted to deliver drugs to target areas of the skin in effective dosages by a variety of means with limited success. Recently, advances in transdermal drug delivery have offered avenues previously unattainable through conventional means. Some of these new delivery methods have been reviewed, explored and offer promising results with the potential to carve a new avenue in the way therapeutic …

A Cellulose-Based Biological Sensor For The Quantitative Determination Of Glucose And Glycated Hemoglobin (Hba1c), Gilberto Henao-Pabon

Open Access Theses & Dissertations

ABSTRACT

Importance: Diabetes is a common world-wide disease characterized by insulin resistance and as result there is an increased risk of cardiovascular disease and other metabolic abnormalities, that contribute to irreversible end-organ damage. The cause of diabetes is multifactorial and includes genetic, inflammatory and environmental factors plus lifestyle issues. Because in diabetes there are high levels of glucose and glycated hemoglobin, these molecules are used in clinical settings for the acute and chronic management of diabetic patients.

Objectives: a. To design and build an electrochemical biological sensor using the enzyme Glucose Oxidase (GOX) to detect glucose in biological fluids.

b. …

The Study Of The Mouse Schlafen Family Members As Mediators Of Antiviral Activity, Julia Fernanda Hernandez

Open Access Theses & Dissertations

The Schlafen family of proteins was first characterized in 1988, and since then evidence of their relevance in different physiological and pathological situations have been accumulating although at a slow pace. Hence, the biological roles of these proteins remain ill-defined. The expression of these proteins is positively regulated by type 1 interferons, constituting a component of the innate immune response. Functions of these proteins vary from regulation of cell proliferation to restriction of viral infections. Recent studies have implicated SLFN11 and SLFN13 as inhibitors of HIV-1 infection2-4. The mechanism of action of SLFN11 resides in its capacity to regulate the …

Design And Characterization Of Thermal Inkjet Bioprinted Constructs For The Treatment Of Diabetic Foot Ulcers And Other Wound Healing Impaired Conditions, Luis Horacio Solis

Open Access Theses & Dissertations

Tissue engineering (TE) is a multidisciplinary practice focused on developing patient-specific constructs to repair, regenerate, or replace injured tissues and organs. One biofabrication process that has gained tremendous momentum in this field is bioprinting. Most, if not all bioprinting modalities embody the same principles of heat and shear due to mechanical forces or friction as cells are forced through narrow orifices. While it is important to examine the interaction between host and implanted constructs, it is also crucial to understand the direct effects that these bioprinting technologies have on cells before embedding them into TE applications.

The purpose of this …

The Study Of Non-Permanent Tattoo Ink Using Nano Silver Compounds And Untact 3d Printing Technology, Jisu Lee

Open Access Theses & Dissertations

Due to a recent epidemic of COVID 19, the possibility of 3D tattoo printing technology using the Internet of Things (IoT) control system developed in a non-face-to-face contact process was identified. A non-surgical tattoo process and hygiene safety differentiated with silver tattoo ink was used to solve the health problems arising from the surgical tattoo process and to identify 3D tattoo printing techniques through a non-personal contact concept. During the tattoo processing, the image selected by the customer is sent to a designated printer via an IoT control system in a separate location. A piston-type extruder (PTE) printing technique was …

Accurate Replication Of An Ear Pinnae Geometry For Use In Acoustic Testing Of Spatial Cues And Sound Localization, Alejandra Belmont

Open Access Theses & Dissertations

Additive manufacturing (AM) enables freedom of design as well as fabrication of complex objects such as the human hear. In medical modeling there is a need for patient-specific customizable parts. The outer human ear consists of these main parts: the pinna, which naturally filters sound, and the ear canal, which is the point at which sound enters before being moved up to the tympanic membrane, otherwise known as the eardrum. In an attempt to accurately replicate ear models, the use of scanning and reverse engineering methods was used. A comparison of 3D laser scanning systems was performed to determine their …

Fabrication, Development, And Characterization Of Hipco Swcnt - Alginate Hydrogel Composites For Cellular Product Applications, Fabian Armando Alvarez-Primo

Open Access Theses & Dissertations

In this study, we designed, synthesized, and characterized ultrahigh purity single-walled carbon nanotube (SWCNT)-alginate hydrogel composites. Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant compositeâ??s efficiency and effectiveness towards biomedical applications. To promote their homogenous dispersion by preventing agglomeration of the SWCNT, at first, we used three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic). After experimentation and corroboration through evidence obtained …

Impact Of Cryptococcus Neoformans Polysaccharide Capsule On Astrocytes, Yeon Hwa Woo

Open Access Theses & Dissertations

Cryptococcus neoformans is a neurotropic, opportunistic, encapsulated, yeast-like fungus that causes life-threatening meningoencephalitis in both healthy and immunocompromised individuals. C. neoformans infects 1,000,000 people and causes 210,000 deaths worldwide annually, mostly in sub-Saharan Africa. The polysaccharide capsule of C. neoformans is the fungus major virulent factor. Glucuronoxylomannan (GXM), the main component of the capsule is abundantly released into brain tissue during C. neoformans infection, causing immunosuppressive defects in the host. C. neoformans is inhaled from the environment, disseminate via bloodstream, and crosses the blood brain barrier (BBB) to cause cryptococcal meningoencephalitis (CM). Although CM causes significant mortality in HIV/AIDS patients, …